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Alexa350 conjugated neutravidin

Manufactured by Thermo Fisher Scientific
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Alexa350-conjugated NeutrAvidin is a fluorescently-labeled protein used in various bioassays and detection applications. It combines the high-affinity binding of NeutrAvidin to biotinylated molecules with the bright Alexa Fluor 350 fluorescent label. This product can be used to detect and localize biotinylated targets in a wide range of research and diagnostic procedures.

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Lab products found in correlation

Ab31940, by Abcam (2 mentions) Goat anti mouse igg2a, by Thermo Fisher Scientific (2 mentions) Vectashield with 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Vector Laboratories (2 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Mms 435, by Fortrea (2 mentions) Fluoromount g, by Southern Biotech (2 mentions)

2 protocols using alexa350 conjugated neutravidin

1

Immunohistochemical Profiling of Neural Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as described (Rich et al., 2018 (link)). Primary antibodies were used against olfactory marker protein (Omp; goat, Wako 019-22291, Research Resource Identifier [RRID]: AB_664696; 1:500), Sox10 (rabbit, gift of Vivian Lee, Medical College of Wisconsin, WI, 1:3,000; Meng, Yuan, & Lee, 2011 ; Yardley & Garcia-Castro, 2012 (link)), Tbr1 (rabbit, Abcam ab31940, RRID: AB_2200219; 1:1,000; expression data summarized in File S3) and Tubb3 (neuronal αIII tubulin; mouse IgG2a, clone TUJ1, Covance MMS-435P, RRID:AB_2313773; 1:250). Matched AlexaFluor-conjugated secondary antibodies (Molecular Probes, Thermo Fisher Scientific) were used at 1:1,000. For triple immunostaining, anti-Tubb3 was detected by a biotinylated secondary antibody (goat antimouse IgG2a, Invitrogen, 1:100), followed by Alexa350-conjugated NeutrAvidin (Molecular Probes, 1:100). Slides were mounted with Fluoromount G (Southern Biotech, Birmingham, AL) or Vectashield with 4’,6-diamidino-2’-phenylindole dihydrochloride (DAPI; Vector Labs, Burlingame, CA).
Perera S.N., Williams R.M., Lyne R., Stubbs O., Buehler D.P., Sauka-Spengler T., Noda M., Micklem G., Southard-Smith E.M, & Baker C.V. (2020). Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia, 68(12), 2550-2584.
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2

Immunohistochemical Profiling of Neural Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as described (Rich et al., 2018 (link)). Primary antibodies were used against olfactory marker protein (Omp; goat, Wako 019-22291, Research Resource Identifier [RRID]: AB_664696; 1:500), Sox10 (rabbit, gift of Vivian Lee, Medical College of Wisconsin, WI, 1:3,000; Meng, Yuan, & Lee, 2011 ; Yardley & Garcia-Castro, 2012 (link)), Tbr1 (rabbit, Abcam ab31940, RRID: AB_2200219; 1:1,000; expression data summarized in File S3) and Tubb3 (neuronal αIII tubulin; mouse IgG2a, clone TUJ1, Covance MMS-435P, RRID:AB_2313773; 1:250). Matched AlexaFluor-conjugated secondary antibodies (Molecular Probes, Thermo Fisher Scientific) were used at 1:1,000. For triple immunostaining, anti-Tubb3 was detected by a biotinylated secondary antibody (goat antimouse IgG2a, Invitrogen, 1:100), followed by Alexa350-conjugated NeutrAvidin (Molecular Probes, 1:100). Slides were mounted with Fluoromount G (Southern Biotech, Birmingham, AL) or Vectashield with 4’,6-diamidino-2’-phenylindole dihydrochloride (DAPI; Vector Labs, Burlingame, CA).
Perera S.N., Williams R.M., Lyne R., Stubbs O., Buehler D.P., Sauka-Spengler T., Noda M., Micklem G., Southard-Smith E.M, & Baker C.V. (2020). Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia, 68(12), 2550-2584.
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