B75442

Flow cytometer analysis was further used to detect the apoptotic cervical cancer cells. Briefly, the cells were exposed to RTF for 24 h and subsequently stained with FITC conjugated Annexin V/PI, and the fluorescence signals were analyzed by a flow cytometer (B75442, Beckman, Pasadena, CA, USA). The percentage of cells in early apoptosis was calculated by Annexin V-positivity and PI-negativity, while the percentage of late apoptotic cells was calculated by Annexin V-positivity and PI-positivity.
In addition, flow cytometry was also performed to analyze the cell cycle arrest of cervical cancer cells induced by RTF treatment for 24 h and subsequently stained with propidium iodide (PI).

K562 cells were collected by centrifugation (300 g, 5 min, room temperature). 5 × 10⁵ cells were used for cell aggregation analysis, and 5 × 10⁵ cells were used to extract nuclei for nucleus aggregation analysis. The labeling procedures for cells and nuclei were like before. For cells and nuclei, 5 and 2.5 µl assembled CASB complex was used, respectively. After labeling, DAPI was added to nuclei suspension according to manufacturer’s instruction. Afterward, cells and nuclei were imaged by fluorescence microscopy (Eclipse Ts2‐FL, Nikon) and analyzed by flow cytometry (B75442, Beckman Coulter).