Anti human cd4 percp cyanine5

Manufactured by Thermo Fisher Scientific

The Anti-human CD4 PerCP-Cyanine5.5 is a fluorescent-labeled antibody that binds to the CD4 antigen on human cells. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cells.

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3 protocols using anti human cd4 percp cyanine5

Multicolor Flow Cytometry for Immune Profiling

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Similar methods of processing of blood samples and flow cytometry have been reported by our previous study (Xu et al. 2018 (link)). Surface staining with antibodies for T cell staining was Anti-human CD4 PerCP-Cyanine5.5 (eBioscience), Anti-human CD185(CXCR5) PE (eBioscience), Anti-human CD183(CXCR3) PE-Cyanine7 (eBioscience), Hu CD196 (CCR6) BV421 11A9 (BD Biosciences) and B cell staining was Hu CD19 PE HIB19, Hu CD27 FITC M-T271 and Hu CD38 BV421 HIT2 from BD Biosciences company.

Yin M., Xiong Y., Liang D., Tang H., Hong Q., Liu G., Zeng J., Lian T., Huang J, & Ni J. (2021). Circulating Tfh cell and subsets distribution are associated with low‐responsiveness to hepatitis B vaccination. Molecular Medicine, 27, 32.

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Multicolor Flow Cytometry for Human Cell Engraftment in Humanized Mice

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To detect human cells engrafted in HUMAMICE, multi-colors cytometric analysis was performed using BD LSR Fortessa, according to the manufacturer’s protocol but with a minor modification. HUMAMICE were sacrificed and the spleens were removed at three different times after hPBMCs were transplanted. Splenocytes were collected and lysed by ACK lysis buffer, counted and incubated with an appropriate volume of antibodies for 1 hour at 4°C and then subjected to flow cytometry analysis.
Commonly used antibodies included: Anti-Human CD45 FITC, Clone: 2D1, (ebioscience9011‐9459); isotype control, Mouse IgG1 K Isotype Control (ebioscience, FITC 11–4714) ; Anti-Mouse CD45 eFluor^® 450 (ebioscience, 48-0451-82) ; isotype control, Mouse IgG2b K Isotype Control eFluor^® 450 (ebioscience, 48-4732-82) ; Anti-Human CD3 APC-eFluor^® 780, Clone: UCHT1, (ebioscience, 47–0038) ; Mouse IgG1 K Isotype Control APC- eFluor^® 780 (ebioscience, 47–4714) ; Anti-Human CD4 PerCP-Cyanine5.5, Clone: OKT4 (OKT-4), (ebioscience, 45–0048); isotype control, Mouse IgG2b K Isotype Control PerCP-Cyanine5.5 (cat. 45–4732) ; Anti-Human CD8a PE-Cyanine7, Clone: SK1, (ebioscience, 25–0087); isotype control, Mouse IgG1 K Isotype Control PE-Cyanine7 (ebioscience, 25–4714) ; Anti-Human CD19 APC, Clone: 2H7, (ebioscience, 17–0209); isotype control, Mouse IgG1 K Isotype Control APC (ebioscience, 17–4714).

Zeng Y., Liu B., Rubio M.T., Wang X., Ojcius D.M., Tang R., Durrbach A., Ru Z., Zhou Y, & Lone Y.C. (2017). Creation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells. PLoS ONE, 12(4), e0173754.

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Cytokine-Induced T Cell Activation

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Anti-human CD3, anti-human CD28, anti-human CD4 PerCP-Cyanine5.5, anti-human CD8a, anti-human CD45RO and anti-human CD45RA antibodies were purchased from eBioscience. Anti-TAGAP and goat anti-rabbit IgG Fc antibodies were purchased from Abcam. Anti-human CCR6 and antihuman CD161 antibodies were purchased from Biolegend Ltd.

Arshad M., Bhatti A., John P., Jalil F., Borghese F., Kawalkowska J.Z., Williams R.O, & Clanchy F.I.L. (2018). T cell activation Rho GTPase activating protein (TAGAP) is upregulated in clinical and experimental arthritis. Cytokine, 104.

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