Annexin 5 fitc pi apoptosis staining kit

Manufactured by Thermo Fisher Scientific

The Annexin V-FITC/PI apoptosis staining kit is a laboratory reagent used to detect and quantify apoptosis in cell samples. It contains Annexin V labeled with the fluorescent dye FITC and the DNA-binding dye propidium iodide (PI). This combination allows for the identification of cells undergoing early and late stages of apoptosis.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions)

Close spelling variants of this product

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2 protocols using annexin 5 fitc pi apoptosis staining kit

Ox-LDL Induced Apoptosis in HUVECs

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HUVECs were incubated with ox-LDL (80 μg/mL, 24 h) and pretreated with CEA for 8 h prior to the apoptosis assay. Double fluorescence staining was performed using an Annexin V-FITC/PI apoptosis staining kit (Molecular Probes™, V13241) according to the manufacturer’s instructions to detect early apoptotic and necrotic cells. Cellular fluorescence was measured using flow cytometry with a FACS Calibur Flow Cytometer (BD Biosciences, USA).

Tian Y., Wang S., Shang H., Wang W.Q., Wang B.Q., Zhang X., Xu X.D., Sun G.B, & Sun X.B. (2019). The clickable activity-based probe of anti-apoptotic calenduloside E. Pharmaceutical Biology, 57(1), 133-139.

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Quantifying Apoptosis in Ox-LDL-Treated HUVECs

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HUVECs were incubated with ox-LDL (70 μg/mL, 24 h) and pretreated with BCEA for 8 h prior to the apoptosis assay. Double fluorescence staining was performed using an Annexin V-FITC/PI apoptosis staining kit (Molecular Probes™, V13241) according to the manufacturer's instructions to detect early apoptotic and necrotic cells. Cellular fluorescence was measured using flow cytometry with a FACS Calibur Flow Cytometer (BD Biosciences, USA).

Wang S., Tian Y., Zhang J.Y., Xu H.B., Zhou P., Wang M., Lu S.B., Luo Y., Wang M., Sun G.B., Xu X.D, & Sun X.B. (2018). Targets Fishing and Identification of Calenduloside E as Hsp90AB1: Design, Synthesis, and Evaluation of Clickable Activity-Based Probe. Frontiers in Pharmacology, 9, 532.

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