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65 protocols using wall mounted stadiometer

1

Comprehensive Cardiometabolic Assessment

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Weight was measured on a Tanita® scale and height with a Seca® wall-mounted stadiometer, according to departmental standard operating procedures. Blood pressure was measured with an automated oscillometric device (Omron®) using a large cuff on the right arm, after participants had been seated quietly for five minutes. Three measures were recorded at one-minute intervals. A 12-lead electrocardiogram was performed to exclude occult ischemic heart disease or cardiac arrhythmia. Bloods were drawn after an overnight fast for glucose, renal and lipid profiles. All blood samples were processed locally in the Galway University Hospitals’ Department of Clinical Biochemistry (certified to ISO 15189 2007 accreditation standard). Glycated haemoglobin (HbA1c) was measured with HPLC (Menarini® HA8160 auto-analyzer). Total cholesterol was measured using the CHOP-PAP method. High density lipoprotein (HDL)-cholesterol and triglycerides were measured using the enzymatic and the GPO-PAP methods, respectively (COBAS® 8000 modular analyzer). Low-density lipoprotein (LDL)-Cholesterol was derived with the Friedewald equation. Information relating to antihypertensive, lipid lowering, and antidiabetic medication use at baseline and at the end of 24 weeks was extracted from the medical records of each participant.
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2

Comprehensive Anthropometric Assessment in Infants

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Anthropometry data will be collected by trained research assistants using standard operating procedures. In order to minimise bias, the measurement team will be blind to group allocation and will be trained to avoid discussing this with parents. Parents will also be advised not to discuss allocation with the measurement team.
Weight will be measured with the baby undressed, using Seca Infant Electronic Scales™ and recorded to the nearest 0.01 kg. For supine length, the baby will placed on a Kiddimeter™ or Starters mat™, with only the nappy on and measured to the nearest 0.5 cm. Abdominal waist circumference will be measured using a D-loop non-stretch fibreglass tape measure and head circumference will be measured using the Child Growth Foundation reusable tape. Sub-scapular, triceps, quadriceps and flank skin fold thickness will be measured using the Holtain Tanner™/Whitehouse™ skin fold calliper with an average of three measurements taken for each site. A standard ultrasound device with a 3C-RS curved transducer will be used to measure intra-abdominal depth and subcutaneous fat.
Parents’ weight and percentage body fat will be measured on a Tanita™ scale and height with a Seca™ wall-mounted stadiometer.
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3

Socio-demographic and Lifestyle Factors Survey

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Questionnaire data regarding socio-demographic variables, history of cancer and other diseases, family history of cancer, reproductive history, HRT and vitamin D supplement use, and current or past smoking behavior were collected from participants at baseline. Information on the subject’s activity level was gathered using a valid physical activity questionnaire [27 (link)] and was then quantified in form of metabolic equivalent hour/day (METs-h/d). This method has been described in detail elsewhere [27 (link), 28 (link)]. Weight was measured using digital scale (Seca, Germany) while the subjects were minimally clothed without shoes and recorded to the nearest 100 g. Height was measured via a wall mounted stadiometer (Seca, Germany) with 2 mm precision, while the participants wearing no shoes. The ratio of weight (in kg) to square of height (in meter) was used to determine the individual’s body mass index (BMI).
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4

Pediatric Growth Measurement Protocol

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Parents filled out a medical history form that included current or past signs and symptoms of disease and drug and/or supplement use. Pubertal development was estimated from parents’ responses to a modified version of the Pubertal Developmental Scale [17 (link)]. Body mass to the nearest 0.01 kg was obtained using a digital scale (ES200L; Ohaus, Pinewood, NJ, USA) with the subject wearing a t-shirt, shorts, and no shoes. Height was measured to the nearest 0.1 cm using a wall-mounted stadiometer (Seca, Ontario, CA, USA) at the end of inhalation. Body mass index (BMI) z-scores were derived from the Centers for Disease Control and Prevention website [18 ].
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5

Anthropometric Measurements Protocol

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Body mass to the nearest 0.01 kg was obtained using a digital scale (ES200L; Ohaus, Pinewood, NJ, USA) with the subject wearing a t-shirt, shorts, and no shoes. Height was measured to the nearest 0.1 cm using a wall-mounted stadiometer (Seca, Ontario, CA, USA) at the end of inhalation. Body mass index (BMI) was derived from dividing body mass in kg by height in meters squared.
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6

Anthropometric Measurements for pQCT Analysis

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Baseline height (cm) was measured to the nearest 1 mm using a wall‐mounted stadiometer (Seca GmbH, Hamburg, Germany) and weight (kg) measured to the nearest 0.1 kg using a digital scale (Seca GmbH) while the participants wore light clothing without footwear. Subsequently, body mass index (BMI; kg/m2) was calculated.
For pQCT, both forearm and lower‐leg length were measured to the nearest 1 mm using a tape measure: tibia length was measured from the distal edge of the medial malleolus to the tibial plateau; ulna length was recorded as the distance from the olecranon to the ulnar styloid process.
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7

Anthropometric Measurements: Height, Weight, and BMI

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Height was measured without shoes with a wall-mounted stadiometer (Seca, Birmingham, UK) to the last completed 0.1 cm after keeping heel, buttocks, back of shoulder, and occiput in the vertical plane and head in the horizontal Frankfurt plane. The weight was measured with minimal light indoor clothing, to the closest 0.1 kg using a calibrated electronic weighing scale (Nagata, BW-110H CAP, Taiwan). BMI was calculated as weight (kg) divided by height (m) squared.
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8

Standardized Clinical Assessment Protocol

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Participants were assessed at Stanford Clinical Translational Research Unit (CTRU). All clinic visits started between 7:00 and 9:30 am, with participants in a fasted state for at least 10-12 h. Participants were also asked to avoid caffeine, alcohol consumption or exercise on the morning of the evaluation. Basic clinical evaluation included measurement of height, weight and vital signs. Body weight was recorded without shoes to the nearest 0.1 kg using a calibrated Scale-tronix clinical scale. Height was measured to the nearest 0.1 cm using a Seca wall-mounted stadiometer. All measurements were taken by a nurse at the Stanford CTRU at each time point. After 5 min of sitting/resting, CTRU nurses obtained three blood pressure readings on the right arm 1 min apart. These were collected automatically using a Welch Allyn, Spot Vital Signs LXi. For analysis purposes, the first blood pressure measurement was disregarded, and the second and third measurements were averaged according to the National
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9

Maternal Factors and Anthropometric Measurements

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Body mass index (BMI) was calculated as body weight divided by squared body height (kg/m2). Body weight was measured to the nearest 0.1 kg with empty pockets and without shoes at each time point by trained professionals using a calibrated balance (SECA, Hamburg, Germany). Body height was measured to nearest 0.1 cm with no shoes on using a wall-mounted stadiometer (SECA, Hamburg, Germany). Standardized questions mainly based on the LifeLines study questionnaires (34 (link)) were employed to gather data on various maternal factors, including age (years), ethnicity (Western/non-Western), marital status (married/living together), parity (no/one or more children), educational level (low/mediate/high), smoking habits (yes/no), and physical activity (MET min/week) and history of gestational diabetes (yes/no). Ethnicity classification was determined based on the participant’s birth country and biological parents and was categorized in Western or non-Western. Education level was divided into low (primary school, vocational or lower general secondary education), mediate (higher secondary education or intermediate vocational training) and high (higher vocational education or university).
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10

Anthropometric Measurements of Athletes

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Subjects reported to the laboratory after an overnight fast and had to abstain from hard training and/or competition for at least 72 h before testing. The participants’ morphological characteristics were measured in the morning and always at the same time (09:00 a.m.). Body height was measured to the nearest 0.1 cm using a wall-mounted stadiometer (Seca©, Hamburg, Germany), and body weight was measured to the nearest 0.01 kg using calibrated electronic digital scales, (Seca©, Hamburg, Germany) in barefoot conditions. Fat mass and fat-free mass content was estimated from the sum of 6 skinfolds (∑6) (abdominal, suprailiac, tricipital and subscapularis, thigh and calf). Skinfold thicknesses were measured with a Harpenden caliper (Holtain Skinfold Caliper, Crosswell, UK) and converted to % of body fat using the equations of Jackson and Pollock [28 (link)]. All measurements were made by the same operator, accredited in kinanthropometric techniques (level 1), in accordance with the International Society for the Advancement of Kinanthropometry (ISAK) recommendations [29 ].
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