E.coli BL21(DE3)pLysS cells were transformed with expression vectors and grown in LB broth (Invitrogen) supplemented with 100 μg/ml ampicillin (Sigma) at 37°C. Protein expression was induced at OD 0.5 with 0.2 mM IPTG (Sigma) and grown at 37°C for 3 hr to overnight. Baculoviruses were produced and amplified in Sf9 cells grown in Grace’s Insect Medium (Gibco) supplemented with 10% fetal bovine serum, 0.1% Poloxamer 188 (Sigma), and 10 μg/ml gentamicin (Gibco) at 27°C. For protein expression, High Five cells were infected with baculoviruses at the density of 5 X 105 cells/ml and grown in Grace’s Insect Medium (Gibco) supplemented with 10% fetal bovine serum, 0.1% Poloxamer 188 (Sigma), and 10 μg/ml gentamicin (Gibco) for 2 days at 27°C.
Poloxamer 188
Manufactured by Merck Group
Sourced in United States, Germany, India, China, France, Brazil
Poloxamer 188 is a nonionic, water-soluble surfactant commonly used in pharmaceutical and biomedical applications. It is a triblock copolymer composed of a central hydrophobic polypropylene oxide (PPO) block flanked by two hydrophilic polyethylene oxide (PEO) blocks. Poloxamer 188 exhibits emulsifying, wetting, and stabilizing properties, making it useful as a dispersing agent, solubilizer, and excipient in various formulations.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Merck Group (17 mentions)
Poloxamer 407, by Merck Group (13 mentions)
Methanol, by Merck Group (10 mentions)
Labrasol, by Gattefosse (8 mentions)
Acetonitrile, by Merck Group (8 mentions)
Ethanol, by Merck Group (7 mentions)
Precirol ato 5, by Gattefosse (5 mentions)
Compritol 888 ato, by Gattefosse (5 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Polysorbate 80, by Merck Group (4 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (4 mentions)
Pepsin, by Merck Group (4 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (4 mentions)
Sodium hydroxide (naoh), by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dichloromethane, by Merck Group (3 mentions)
Transcutol hp, by Gattefosse (3 mentions)
Symplicity system, by Merck Group (3 mentions)
Pcl pvac peg, by BASF (3 mentions)
Peg ppg peg, by Merck Group (3 mentions)
114 protocols using poloxamer 188
1
Recombinant Protein Expression in E. coli and Insect Cells
2
Pioglitazone Hydrochloride Formulation Development
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Pioglitazone hydrochloride (PGZ) drug was received as a gift sample from Xellia Pharmaceuticals. Methanol, Tween 80, poloxamer 188, Span 80, Compritol® 888 ATO, and Labrasol® were procured from Sigma-Aldrich (St. Louis, MO, United States). Analytical-grade quality was assured for all chemicals.
3
TMEM16 Protein Expression in HEK293 Cells
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HEK293T cells (ATCC CRL-1573) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10 U ml−1 penicillin, 0.1 mg ml−1 streptomycin (Sigma-Aldrich), 2 mM L-glutamine (Sigma-Aldrich), and 10% FBS (Sigma-Aldrich) in a humidified atmosphere containing 5% CO2 at 37 °C. HEK293S GnTI− cells (ATCC CRL-3022) were maintained in HyClone HyCell TransFx-H medium (GE Healthcare) supplemented with 10 U ml−1 penicillin, 0.1 mg ml−1 streptomycin, 4 mM L-glutamine, 0.15% poloxamer 188 (Sigma-Aldrich), and 1% FBS in an atmosphere containing 5% CO2 at 185 rpm at 37 °C. The ac splice variant of mouse TMEM16A (UniProt ID: Q8BHY3), mouse TMEM16B (UniProt ID: Q8CFW1), or mouse TMEM16F (UniProt ID: Q6P9J9) bearing a 3C cleavage site, a Venus YFP, a Myc tag, and a Streptavidin-binding peptide (SBP) downstream of the open reading frame in a modified pcDNA3.1 vector (Invitrogen) were used as described previously21 (link),31 . Mutations were introduced using a modified QuikChange method58 (link) and were verified by sequencing.
4
Microfluidic Double Emulsion Fabrication
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Three solutions were used to produce
double emulsions on-chip: a lipid-containing solution, an inner aqueous
solution, and an outer aqueous solution. The lipid-containing solution
was composed of 2 mg mL–1 lipids (99.9 mol
% DOPC + 0.1 mol % Liss Rhod PE) dissolved in oleic acid. In
all of the experiments involving double emulsions, both inner and
outer aqueous and the feeding channel solutions contained 5% v/v pluronic
surfactant (poloxamer 188, Sigma-Aldrich) and 15% v/v glycerol. For
the experiments shown inFigure 4 , the inner aqueous and outer aqueous contained an
additional 25 mM sucrose and 5 mM MgCl2. The solution flushed
through
the feeding channel to induce an osmotic pressure difference contained
an additional 200 mM sucrose. To make the double emulsion denser than
the surrounding solution and thereby facilitate their extraction from
the device (Figure 5 ), an additional 5 mM dextran was added to the inner aqueous solution
and osmotically balanced by 5 mM of glucose in the outer aqueous solution.
double emulsions on-chip: a lipid-containing solution, an inner aqueous
solution, and an outer aqueous solution. The lipid-containing solution
was composed of 2 mg mL–1 lipids (99.9 mol
% DOPC + 0.1 mol % Liss Rhod PE) dissolved in oleic acid. In
all of the experiments involving double emulsions, both inner and
outer aqueous and the feeding channel solutions contained 5% v/v pluronic
surfactant (poloxamer 188, Sigma-Aldrich) and 15% v/v glycerol. For
the experiments shown in
additional 25 mM sucrose and 5 mM MgCl2. The solution flushed
through
the feeding channel to induce an osmotic pressure difference contained
an additional 200 mM sucrose. To make the double emulsion denser than
the surrounding solution and thereby facilitate their extraction from
the device (
and osmotically balanced by 5 mM of glucose in the outer aqueous solution.
5
Rutin-Eudragit S100 Oral Formulation
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Rutin was provided as a kind gift sample from Kahira Pharmaceuticals and Chemical industries Co. (Cairo, Egypt). Eudragit S100 was purchased from Evonik industries (Marl, Germany). Poloxamer-188 and methanol were procured from Sigma-Aldrich Co. (St. Louis, MO, USA). All other chemical reagents used in this study were of analytical grade.
6
Gelatin-Based Hydrogel Characterization
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Gelatin B (average Mw 20 to 25 kDa, bloom strength 75 g), Poloxamer 188, 25% aqueous glutaraldehyde solution, branched polyethyleneimine 25 kDa (bPEI) and solvents have been purchased from Sigma Aldrich (Steinheim, Germany). Sodium metabisulfite was purchased from Merck (Darmstadt, Germany). Fluorescein isothiocyanate-dextran 150 kDa (FITC-dextran) was derived from TdB (Uppsala, Sweden). RPMI-1640 cell culture medium, HBSS buffer and 4′,6-Diamidino-2-phenylindol solution (DAPI) were obtained from Sigma Aldrich LifeScience GmbH (Seelze, Germany). Fetal calf serum (FCS) was purchased from Lonza, (Basel, Switzerland) and glutamine from Gibco–Thermo Fisher Scientific (Darmstadt, Germany). IbiTreat® microscopy chambers were derived from Ibidi GmbH (Martinsried, Germany). Wheat Germ Agglutinin Alexa Fluor™ 633 conjugate (WGA Alexa Fluor™ 633) was purchased from Thermo Fisher Scientific Inc. (Darmstadt, Germany). Mouse and Human Interleukin 6 (IL-6) as well as Mouse and Human Tumor Necrosis Factor Alpha (TNF-α) ELISA kits were purchased from Shanghai Korain Biotech (Shanghai, China).
7
Terbinafine HCl Formulation Development
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Terbinafine HCl was obtained from the Novartis Research Institute in Vienna, Austria. Polymers such as Poloxamer-188 and Poloxamer-407 were sourced from Sigma-Aldrich in Darmstadt, Germany. The other materials used are of analytical grade.
8
Optimized Encapsulation of ENR Powder
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ENR standard (98% ENR content) was bought from Dr Ehrenstorfer. ENR (≥96.0% ENR content) was purchased from Wuhan Konglong Century Technology Development Co., Ltd. (Wuhan, China). ENR soluble powder was purchased from Guangdong Wens Dahuanong Biotechnology Co., Ltd. (Guangdong, China). Octadecanoic acid and docosanoic acid were obtained from Aladdin (Shanghai, China). Polyvinyl alcohol (PVA) and poloxamer 188 were bought from Sigma (St. Louis, MO, USA). Polyvinyl pyrrolidone (PVPK30) was obtained from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). Polyacryl resin II (PRII) was purchased from Shanghai Source Biological Technology Co., Ltd (Shanghai, China). Ethyl cellulose (EC) and sodium carboxymethyl cellulose were purchased from Sinopharm Group Chemical Reagent Co., Ltd.
9
Characterization of Antioxidant Compounds
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LE (MW =1,045.71, purity ≥98%) was acquired from Shanghai Tongtian Biotechnology Co., Ltd. (Shanghai, China). Lutein (MW =568.87, purity =99%) was acquired from Hubei Yuancheng Science and Technology Co., Ltd. (Hubei, China). Poloxamer 188, ethanol, acetonitrile, methanol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium ferricyanide, FeCl3, NaH2PO4·2H2O, ethyl acetate, trichloromethane, n-hexane, acetone, petroleum ether, tetrahydrofuran, and dichloromethane were purchased from Sigma. Deionized water was obtained using Hitech-K flow water purification system (Hitech Instruments Co., Ltd., Shanghai, China).
Kunming strain mice (18–22 g, male) and Sprague Dawley rats (weight, 180–220 g) were obtained from Beijing HFK Bioscience Co., Ltd. (Beijing, China) and were used as subjects in the experiment. All animal operations were implemented in accordance with the Guidelines for the Care and Use of Experimental Animals of Harbin Medical University. Animal experiments were approved by the Ethics Committee of the Harbin Medical University.
Kunming strain mice (18–22 g, male) and Sprague Dawley rats (weight, 180–220 g) were obtained from Beijing HFK Bioscience Co., Ltd. (Beijing, China) and were used as subjects in the experiment. All animal operations were implemented in accordance with the Guidelines for the Care and Use of Experimental Animals of Harbin Medical University. Animal experiments were approved by the Ethics Committee of the Harbin Medical University.
10
Cyclodextrin-based Dissolution Enhancement
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DEX reference standard was gifted from SAMI Pharmaceutical (Pvt) LTD., Karachi (Pakistan). Hydroxypropyl β-cyclodextrin (HPβCD) (1380 gm molecular weight, 278 °C melting point and 0.6 molar substitution) was supplied by Roquette (Lestrem, France). Poloxamer-188 (PXM-188) and poloxamer-407 were bought from Sigma-Aldrich (St. Louis, MO, USA). Ethanol 99% (35%) was bought from British Drug Houses (BDH). Distilled water was provided by pharmaceutical lab, faculty of pharmaceutical sciences, GCUF. All solvents and chemicals were of analytical grade.
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