For the scanning electron microscopy (SEM), seedlings were fixed in 3.7% formaldehyde, 50% ethanol, and 5% acetic acid by vacuum infiltration for 30 min. Seedlings were later kept in the fixative for 8 h, followed by a slow dehydration through a series of ethanol concentrations: 50%, 70% and 90%, 100%, 100%, 60 min each. Seedlings were critical-point dried in liquid CO2 in a Quorum K850 critical-point dryer (Quorum Technologies, East Sussex, UK), and sputter-coated with gold palladium using a Quorum SC7620 mini sputter coater (Quorum Technologies, East Sussex, UK). Images were taken with a JEOL JCM6000 benchtop SEM (Jeol, Japan).
Quorum sc7620 mini sputter coater
Manufactured by Quorum Technologies
Sourced in United Kingdom
The Quorum SC7620 mini sputter coater is a compact device designed for the deposition of thin conductive coatings onto sample surfaces. It is primarily used to prepare samples for examination under scanning electron microscopes (SEM) or transmission electron microscopes (TEM).
Automatically generated - may contain errors
2 protocols using quorum sc7620 mini sputter coater
1
Seedling Preparation for SEM Imaging
2
Scanning Electron Microscopy of Plant Tissues
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For scanning electron microscopy (SEM), leaflets and meristems were fixed in 3.7% formaldehyde, 50% ethanol, and 5% acetic acid by vacuum infiltration for 30 min and an additional 8 h in the fixative. The samples were dehydrated in a graded ethanol series (50%, 70%, and 90%, 100%, 100%, 60 min each). Dehydrated tissues were critical-point dried in liquid CO2 in a Quorum K850 critical-point dryer (Quorum Technologies, East Sussex, UK), and sputter-coated with gold-palladium (QuorumSC7620 mini sputter coater). Images were taken with a JEOL JCM-6000 benchtop SEM device.
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