Receiver operating characteristic (ROC) analyses were performed with MedCalc (version 19.2.1); statistical analyses (calculation of 95% confidence intervals [CIs] according to the modified Wald method and Fisher’s exact test) were done using GraphPad Prism QuickCalcs. To avoid statistical bias, a maximum of 1 sample per patient per category was included for calculations of diagnostic and analytical sensitivities. If several samples from an individual patient belonged to the same category, the sample taken on the latest day after the onset of symptoms was included in the analysis.
Prism quickcalcs
Manufactured by GraphPad
Sourced in United States
Prism QuickCalcs is a free online calculator tool provided by GraphPad. It allows users to perform various statistical and analytical calculations quickly and easily. The tool covers a range of common calculations, including t-tests, ANOVA, correlation, and more.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using prism quickcalcs
1
ROC Analysis for Diagnostic Sensitivity
2
Statistical Analysis of Diagnostic Tests
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Statistical analysis was performed using Medcalc stats (https://www.medcalc.org/calc/diagnostic_test.php ), and Cohen’s kappa coefficient (κ) was calculated using the Graphpad Prism QuickCalcs website (https://www.graphpad.com/quickcalcs/kappa1/ ).10 The independent sample t‐test was used to determine statistically significant differences of independent samples in two groups using IBM SPSS statistics version 19.
3
Statistical Analysis of Experimental Findings
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The statistical analysis of the results was performed with the student T-test and Welch’s correction test, when appropriate (GraphPad Prism quickCalcs, San Diego, CA, USA). The differences were considered significant for a p value < 0.05.
4
Statistical Analysis of Research Protocols
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Statistical analysis, including for positive and negative agreement, overall concordance and kappa coefficients were done using GraphPad Prism QuickCalcs (https://www.graphpad.com/quickcalcs/ ).
5
Statistical Analyses and Data Visualization Protocols
6
Assessing PLA2R1-ab Detection Methods
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The data were evaluated statistically using GraphPad Prism 6, GraphPad Prism QuickCalcs (GraphPad Software Inc., La Jolla, CA) and SigmaPlot 13.0 (SSI, San Jose, CA). Kappa statistic (κ) and its 95% confidence interval were used to assess the level of agreement of PLA2R1-ab detection between ChLIA and RC-IFA or ELISA.29 (link)
7
Multiparametric Analysis of Mitochondrial Function
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All statistical analyses and P value determinations were conducted using GraphPad Prism QuickCalcs or GraphPad Prism 9 (version 9.2.0, GraphPad Software). For P values in multiple comparisons (two-tailed unpaired t test; one or two-way ANOVA), Tukey’s multiple comparisons test was performed in GraphPad Prism 9. Scatter plots for TMRE, L-lactate assays were plotted with GraphPad Prism 9. ECAR and OCR curves, along with the SD, and live-cell actin burst, along with the SEM were all plotted using Microsoft Excel for Office 365 (version 16.0.11231.20164, Microsoft Corporation). Exact P values, sample size N, and the number of independent experiments for each analysis are provided in Table S1 . For all experiments, the data distribution was assumed to be normal, but this was not formally tested.
8
Evaluating Root Morphology and Hair Traits
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After seven DAG the plates were scanned and total root length and gravitropic index were evaluated by using the ImageJ program from NIH. The gravitropic index was calculated according to [50 (link)]. Root tip bright field pictures for the evaluation of root hair traits were taken under the Zeiss880 microscope using the 20× objective and grabbing 3 × 9 tiles under 2× zoom, to capture 2 cm of the root tip. The root hair traits were also evaluated by using Image J. Distance to first root hair was calculated from the root tip to the first emerging root hair. Elongating root hairs were only taken into consideration when root hair length was measured. A summary of all average values with standard error and standard deviation is added in the Supplementary Tables S1 and S2 ). Statistical significance was assessed through unpaired Student’s t-tests for comparisons between two conditions (normally distributed data, GraphPad Prism QuickCalcs was used https://www.graphpad.com/quickcalcs/ttest1/ ) or Mann–Whitney U test (not normally distributed data, online tool—https://www.statskingdom.com/170median_mann_whitney.html ); when more groups were evaluated against a control, Kruskal–Wallis test with Dunn’s post hoc test (online tool—https://astatsa.com/KruskalWallisTest/ ) was done. A summary of all average values with standard error and standard deviation is added in the Supplementary Figure S1 .
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