Liver toxicity was measured using C57B6/J mice (male 8 weeks), which were dosed with LNPs encapsulating Cre mRNA (LNP-Cre) at 0.05 or 0.1 mg/kg body weight or dosed with DPBS. At 24 h after administration, blood samples were collected from the jugular vein. The ALT and AST levels were measured with L type WAKO ALT J2 and L type WAKO AST-J2 (FUJIFILM Wako Pure Chemical, Osaka, Japan) according to the manufacturer’s instructions.
L type wako alt j2
Manufactured by Fujifilm
Sourced in Japan
The L type WAKO ALT J2 is a laboratory equipment product designed for specific analytical functions. It provides core measurement capabilities without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
L type wako ast j2, by Fujifilm (4 mentions)
Model 7180, by Hitachi (1 mentions)
Mouse ferritin elisa kit, by Abcam (1 mentions)
Xt 2000iv, by Sysmex (1 mentions)
Celltac α, by Nihon Kohden (1 mentions)
Wako l type tg m, by Fujifilm (1 mentions)
L type wako cho m, by Fujifilm (1 mentions)
Quick auto neo glu hk, by Shino-Test (1 mentions)
5 protocols using l type wako alt j2
1
Evaluating Liver Toxicity of Cre mRNA LNPs
2
Comprehensive Hematological and Liver Biomarker Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Under isoflurane anesthesia, blood samples were drawn from the hearts of mice into a syringe with or without 10% EDTA-2K solution as an anticoagulant. The hematological parameters were determined with an automated cell counter (XT-2000iV, Sysmex Co., Ltd., Kobe, Japan). Plasma or serum was obtained after 30 min of centrifugation at 3,000 rpm. Using the Mouse Ferritin ELISA Kit (ab157713; Abcam) and a microplate reader (SpectraMax M5), plasma ferritin levels were determined. Serum aspartate aminotransferase, alanine aminotransferase, and total bilirubin levels were determined using L-type Wako AST・J2, L-type Wako ALT・J2 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), and Nascauto VL T-BIL (Alfresa Pharma, Ohsaka, Japan), respectively and an auto-analyzer (Model 7180, Hitachi, Tokyo, Japan).
3
Comprehensive Blood and Organ Dysfunction Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Platelets were counted using an automated device for animals (cat. no. MEK-6558; Celltac α; Nihon Kohden Co.) within 1 h of sampling. Citrated plasma samples obtained by whole-blood centrifugation were stored at -80˚C until required. Fibrinogen concentration and prothrombin time (PT) were determined using a clotting assays according to the manufacturer's protocol (Fibrinogen determination and Thromborel S; Sysmex Co.). D-dimer levels were determined using a quantitative latex agglutination test according to the manufacturer's protocol (ELPIA ACE DD dimer; LSI Medience). Plasma levels of TNF were measured using a rat ELISA kit (cat. no. MBS2507393; BioSource). To determine the extent of organ dysfunction in rats, the plasma levels of creatinine and alanine aminotransferase (ALT) were determined using enzymatic (PureautoS CRE-L; cat. no. 20800AMZ10178000; Sekisui Medical Co.) and ultraviolet (Ltypewako ALT J2; FUJIFILM Wako Pure Chemical Corporation) determinations according to the manufacturer's protocol, respectively.
4
Fasting Serum Biochemical Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected by cardiocentesis from isoflurane anesthetized mice at 11 Am after 15 hours’ fasting and used for serum preparation. Serum levels of glucose, immunoreactive insulin, triglycerides (TGs), total cholesterol (T-Cho), aspartate transaminase (AST), and alanine transaminase (ALT) were measured using the Quick-auto-neo-GLU-HK (Shino-Test), Insulin Rat enzyme-linked immunosorbent assay (ELISA) kit (catalog No. ERINS, RRID: AB_2848197, Thermo Fisher Scientific), L-type Wako TG M, L-type Wako CHO M, L-type Wako AST J2, and L-type Wako ALT J2 (Fujifilm-Wako), respectively.
5
Serum Biomarker Measurement in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anesthetized rats underwent thoracotomy and heart puncture to collect 3 mL of whole blood. Blood was collected in empty tubes and centrifuged at 805× g for 10 min. After centrifugation, serum was collected and sent to the Oriental Yeast Industry Co., Ltd. (Shiga, Kyoto, Japan) for measurements of four substances in the blood: ALT, AST, ALP, and T-BIL. AST, ALT, and ALP were determined using the JCCLS standard for Molecular Methods (L Type-Wako AST J2, L Type-Wako ALT J2, Type-Wako ALP J2, FUJIFILM Wako Chemicals, Osaka, Japan). T-BIL levels were determined by enzymatic analysis (Nescauto VL T-BIL, Alfresa Pharma Corporation, Osaka, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!