1260 hip degasser

The phenolic compounds were extracted from semolina, olive paste flour (OPF), spaghetti CTRL and spaghetti enriched with 10% OPF in two separated fractions: soluble free and bound, following the method reported by Mattilla [20 (link)] without acid hydrolysis. The difference between total and free polyphenols represented the bound phenolic compounds. HPLC-DAD analysis was performed using the Agilent 1260 infinity system, equipped with a 1260 binary pump, 1260 HiP Degasser, 1260 TCC Thermostat, 1260 Diode Array Detector and Agilent Open Lab Chem Station Rev C.01.05 (35) software. The UV–visible absorption chromatogram was detected at 280 nm, 325 nm and 360 nm. The separation was performed by gradient elution on a 4.6 × 250 mm reversed phase Luna C-18 (5 μm) column (Phenomenex Torrance, California, USA). The elution was performed using methanol (eluent A) and water/acetic acid 95:5 (eluent B). The gradient profile was: 85–60% B (0–25 min), 60% B (25–30 min), 60–37% B (30–45 min), 37% B (45–47 min), 37–0% B (47–52 min). The flow rate was 1 mL/min and the injection volume was 25 μL. Phenolics were identified and quantified by the retention time, spectra and response factors of the pure standards.