Rabbit anti-phospho-Chk1 (pS345, #2341), anti-phospho-Chk2 (pT68, #2661), anti-H2AX (#2595), anti-Chk1 (#2360), anti-Chk2 (#3440), anti-UBC13 (#4919S) and mouse anti-Cyclin E (#4129) were purchased from Cell Signaling Technology. Rabbit anti-CtIP (A300–488A) and anti-phospho-RPA2 (p4/p8, A300-245A) were purchased from Bethyl Laboratories. Mouse anti-phospho-H2AX (05-636) and anti-RPA2 (04-1481) were purchased from Millipore. Mouse anti-Actin (ab8227), anti-RAD52 (ab18264) and anti-RAD54B (ab137584) were purchased from Abcam, and anti-green fluorescent protein (GFP; 632381) was purchased from Clontech Laboratories. Anti-RAD51 (sc-8349) and anti-XRCC2 (sc-5895) were purchased from Santa Cruz Biotechnology. Rabbit anti-SERBP1 antibody was raised against a peptide containing the SERBP1 sequence (186-SRGKREFDRHSGSDRS-202).
Anti rad51 sc 8349
Manufactured by Santa Cruz Biotechnology
Anti-RAD51 (sc-8349) is a primary antibody that recognizes the RAD51 protein. RAD51 is a key player in the DNA repair pathway known as homologous recombination. The antibody can be used in various laboratory techniques to detect and study the RAD51 protein.
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Lab products found in correlation
Mouse anti cyclin e 4129, by Cell Signaling Technology (1 mentions)
Anti γh2ax, by Merck Group (1 mentions)
Anti h2a, by Merck Group (1 mentions)
Anti 53bp1 sc 22760, by Santa Cruz Biotechnology (1 mentions)
Anti fancd2 nb100 182, by Novus Biologicals (1 mentions)
Bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti pten, by Cell Signaling Technology (1 mentions)
Eclipse ti e fluorescence microscope, by Nikon (1 mentions)
3 protocols using anti rad51 sc 8349
1
Antibody Panel for DNA Damage Response
2
Immunoblotting Analysis of DNA Repair Proteins
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For immunoblotting analysis, cell suspensions were washed in ice-cold PBS and lysed in 50 mM Tris.Cl pH 7.4, 1.0% v/v NP-40, 0.25% w/v Deoxycholic acid, 150 mM NaCl, 1 mM EGTA, 1 mM PMSF, 1 mM Na3O4V, 1 mM NaF, plus protease inhibitors cocktail (Roche). The cellular fractionation method is described in the Supplemental Experimental Procedures . Proteins were resolved on NuPAGE 3–8% w/v Tris-Acetate or 4–12% w/v Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF) membranes. The following antibodies were used: rabbit polyclonal anti-53BP1 (sc-22760; Santa Cruz Biotechnology), anti-FANCA (ABP6201; Cascade), anti-FANCD2 (NB100–182; Novus Biologicals), anti-FANCI (Dr. Patrick Sung, Yale University and A301-254A; Bethyl Laboratories), anti-FANCM (a kind gift from Dr. Ruhikanta Meetei, Cincinnati Children’s Hospital), anti-FANCM (3821; Fanconi Anemia Research Fund), anti-H2A (07–146; Millipore), and anti-PTEN (9559;Cell Signaling), and mouse monoclonal anti-FANCM (CP3.2, CV11.1, and CV5.1), anti-γH2AX (05–636;Millipore), anti-PTEN (6H2.1;Cascade), anti-RAD51 (sc-8349; Santa Cruz), and anti-α-tubulin (MS-581-PO; Lab Vision).
3
Immunofluorescence Staining of Rad51 and Rad54
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Cell were treated with 1% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X-100 for 15 min. Cells were then blocked with 3% bovine serum albumin (BSA) in PBS with 0.1% Tween 20 (PBST) for 30 min and incubated for 1 h with the following primary antibodies, diluted at the indicated ratios in 3% BSA in PBST: anti-Rad51 (sc-8349, Santa Cruz) and anti-Rad54 (sc-374598, Santa Cruz). After three washes with PBST, the cells were incubated for 1 h with appropriate Alexa 488-, fluorescein isothiocyanate (FITC)-, Cy3-, or Cy5-conjugated secondary antibodies and then mounted by means of a mounting solution containing 4′,6-diamidino-2-phenylindole (DAPI). Images were captured using the Eclipse Ti-E fluorescence microscope (Nikon, Tokyo, Japan) with a 100× objective lens (NA 1.40).
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