Two well chamber slides

Manufactured by BD

Sourced in United States

The two-well chamber slides are a type of laboratory equipment designed for cell culture applications. These slides feature two separate chambers, allowing researchers to culture and observe cells in a controlled environment. The core function of these slides is to provide a platform for culturing and studying cells in a convenient and reproducible manner.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Turbofectin 8.0 transfection reagent, by OriGene (1 mentions) Alexa 555 conjugated anti mouse secondary antibody, by Cell Signaling Technology (1 mentions) Dm4000b, by Leica (1 mentions)

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Chamber slides (78 citations)

2 protocols using two well chamber slides

Characterization of HCT-116 Colon Cancer Cells

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Human colon cancer cells HCT-116, p53-wild type (WT) or p53-null, generated by targeted disruption of the p53 alleles in parental HCT116 cells, were originally obtained by B. Vogelstein, Johns Hopkins University School of Medicine (28 (link)). The cells were a gift to Dr Carleen Cullinane at Peter MacCallum Cancer Centre in Melbourne and then passed to the OAM group. The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) complemented with 10% Foetal Bovine Serum (FBS), 2.0 ml-glutamine, 100 U/ml Penicillin and 100 µg/ml Streptomycin (all reagents from Life Technologies, Australia). Cell cultures were maintained at 37°C in a humidified environment of 5% CO₂. The cells were plated in two-well chamber slides (BD, Franklin Lakes, NJ, USA) and incubated overnight prior to irradiation and irradiated at 75-80% confluency.

Lobachevsky P., Forrester H.B., Ivashkevich A., Mason J., Stevenson A.W., Hall C.J., Sprung C.N., Djonov V.G, & Martin O.A. (2021). Synchrotron X-Ray Radiation-Induced Bystander Effect: An Impact of the Scattered Radiation, Distance From the Irradiated Site and p53 Cell Status. Frontiers in Oncology, 11, 685598.

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Confirming H7HA Gene Expression by Indirect Immunofluorescence

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An indirect immunofluorescent assay was utilized to confirm H7HA gene expression as described previously [35 (link)]. Briefly, human rhabdomyosarcoma (RD) cells were plated on two-well chamber slides (BD Biosciences), at a density to obtain 60-70% confluency the next day in complete DMEM medium with 10% FBS (GIBCO) and allowed to adhere overnight. The cells were transfected with pH7HA and the control plasmid pGX0001 (1 ug/well) using TurboFectin^™8.0 Transfection Reagent (OriGene) according to the manufacturer’s instructions. Forty-eight hours later, the cells were washed gently three times with 1XPBS and fixed on slides using ice cold methanol for 10 min. The cells were incubated with anti-H7N9 HA mouse monoclonal antibody (Sino Biological Inc., Cat# 11082-MM04) at a 1:400 dilution for 2h at room temperature. The slides were then incubated with the Alexa 555-conjugated anti-mouse secondary antibody (Cell Signaling Technology) for 60 min in the dark, and analyzed by fluorescent microscopy (Leica DM4000B, Leica Microsystems Inc, USA) using the SPOT Advanced software program (SPOT^™ Diagnostic Instruments, Inc).

Yan J., Villarreal D.O., Racine T., Chu J.S., Walters J.N., Morrow M.P., Khan A.S., Sardesai N.Y., Kim J.J., Kobinger G.P, & Weiner D.B. (2014). Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine. Vaccine, 32(24), 2833-2842.

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