Serum (for total cholesterol, LDL, HDL, and triglycerides) and plasma (for all other measures) were isolated from cord blood by centrifugation as soon as possible after collection. Samples were stored at -80°C until analysis. The following biochemical measures were obtained: plasma glucose levels (hexokinase method, Sigma); adiponectin (Linco Human Adiponectin ELISA kit, Millipore); leptin (Linco Human Leptin ELISA kit, Millipore); C-peptide ( Human C-peptide Radioimmunoassay, Millipore); insulin (fluoroimmunoassay, Beckman Coulter); Non-esterified fatty acid (NEFA) levels (enzymatic colorimetric method assay, Wako); total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels (Beckman Coulter Unicel® DxC 800 system, timed endpoint methods).
Human c peptide radioimmunoassay
Manufactured by Merck Group
The Human C-peptide Radioimmunoassay is a laboratory test used to measure the concentration of C-peptide, a byproduct of insulin production, in human blood samples. The assay employs radioisotope-labeled C-peptide and anti-C-peptide antibodies to quantify the C-peptide levels.
Automatically generated - may contain errors
2 protocols using human c peptide radioimmunoassay
1
Cord Blood Metabolic Biomarkers
2
Cord Blood Metabolic Biomarkers
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