O. maius was grown at 25°C in plastic (6 replicates) and glass (3 replicates) petri plates containing MS medium covered with cellophane membranes. VOCs were collected in the cultures headspace after 15 and 30 days from fungal inoculum. Control plates without mycelium were sampled for background correction. VOCs were collected for 6 h from sealed Petri dishes by headspace sorptive extraction using the stir bar sorptive extraction method with Gerstel Twisters (Gerstel GmbH & Co. KG, Mülheim an der Ruhr, Germany) as described in [33 (link)]. The samples were analysed with a thermo-desorption unit (Gerstel GmbH & Co) coupled to a gas chromatograph-mass spectrometer (GC-MS; GC model: 7890A; MS model: 5975C; Agilent Technologies, Santa Clara, CA, USA) as described in [34 (link)]. The chromatograms were analyzed by the enhanced ChemStation software (MSD ChemStation E.02.01.1177, 1989–2010 Agilent Technologies, Santa Clara, CA, USA). The TIC (Total Inorganic Carbon) of each VOC in the final dataset was recalculated from the absolute abundance of the first representative m/z to eliminate noise. The calibration was done as described in [34 (link)]. The emission rates were calculated on fungal mycelium area (pmol cm-2 h-1) bases.
Gc model 7890a
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A Gas Chromatograph (GC) is a laboratory instrument used for the separation, identification, and quantification of chemical compounds in a sample. It features an inert flow path, electronic pneumatic controls, and a wide selection of detectors to enable sensitive and reliable analysis of a variety of samples.
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Lab products found in correlation
Model 5975c ms, by Agilent Technologies (1 mentions)
Thermo desorption unit, by Gerstel (1 mentions)
Msd chemstation e 0201, by Agilent Technologies (1 mentions)
Novaspec 2, by GE Healthcare (1 mentions)
Durabond db waxetr column, by Agilent Technologies (1 mentions)
Tensor 2, by Bruker (1 mentions)
Db 225 ms capillary column, by Agilent Technologies (1 mentions)
8 protocols using gc model 7890a
1
Headspace Sorptive Extraction of Fungal VOCs
2
Analytical Methods for Biofuel Production
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Cell density was measured by monitoring the optical density (OD) at 600 nm with a visible spectrophotometer (Novaspec II, Pharmacia Biotech, Cambridge, England).
Glucose, lactate, acetate, and butyrate were quantified by High Performance Liquid Chromatography (HPLC) (Agilent Technologies, USA) equipped with a refractive index detector (RID) and a variable wavelength detector (VWD, 210 nm). A Bio-Rad Aminex HPX-87H column (300 mm × 7.8 mm) was used. Five micromolar of sulfuric acid was used as the mobile phase at the flow rate of 0.7 mL/min.
Butanol, acetone, ethanol, and isopropanol were quantified by gas chromatography (GC, model 7890A; Agilent Technologies, USA) equipped with a Durabond (DB)-WAXetr column (30 m × 0.25 mm × 0.25 μm, J&W, USA) and a flame ionization detector (FID). The oven temperature was initially maintained at 60 °C for 2 min, increased at 15 °C/min to 230 °C, and held at 230 °C for 1.7 min. Helium was used as the carrier gas with a column flow rate of 1.5 mL/min (Chua et al., 2013 (link)).
Glucose, lactate, acetate, and butyrate were quantified by High Performance Liquid Chromatography (HPLC) (Agilent Technologies, USA) equipped with a refractive index detector (RID) and a variable wavelength detector (VWD, 210 nm). A Bio-Rad Aminex HPX-87H column (300 mm × 7.8 mm) was used. Five micromolar of sulfuric acid was used as the mobile phase at the flow rate of 0.7 mL/min.
Butanol, acetone, ethanol, and isopropanol were quantified by gas chromatography (GC, model 7890A; Agilent Technologies, USA) equipped with a Durabond (DB)-WAXetr column (30 m × 0.25 mm × 0.25 μm, J&W, USA) and a flame ionization detector (FID). The oven temperature was initially maintained at 60 °C for 2 min, increased at 15 °C/min to 230 °C, and held at 230 °C for 1.7 min. Helium was used as the carrier gas with a column flow rate of 1.5 mL/min (Chua et al., 2013 (link)).
3
Spectroscopic Analysis of Biomolecules
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Fourier-transform infrared spectroscopy (FTIR) was carried out with a Perkin–Elmer FTIR instrument (Tensor II, Bruker, Germany). The spectral range was 4000 to 400 cm−1. Gas chromatography/mass spectrometry (GC/MS) was performed with an Agilent instrument (GC model 7890A; MS model 5977B; Agilent Technologies, Santa Clara, CA) with a DB-225 ms capillary column (30 m; 0.25 mm internal diameter; 0.25 μm film thickness) (Keramat, Golmakani, Durand, Villeneuve, & Hosseini, 2021 (link)). Carbon nuclear magnetic resonance (13C NMR), and hydrogen nuclear magnetic resonance (1H NMR) spectral data were determined on a 400 MHz (Bruker Avance-Ш, Karlsruhe, Germany) spectrometer at 25°.
4
Photocatalytic Oxidation of Cyclohexane
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Example 4
Photocatalytic Oxidation of Cyclohexane: Experimental
Photocatalytic experiments were performed by feeding a N2 stream at 30 L/h (STP) containing 200 ppm cyclohexane, 10 vol. % O2 at a temperature of 60° C. and a reaction pressure of 1 atm. Nitrogen functioned as the carrier gas for cyclohexane. Additionally, 320 ppm of water vaporized from 60° C. controlled saturators was added in order to minimize photodeactivation of the catalyst. A fluidized bed photoreactor was used as the reactor, which was irradiated by a Xenon lamp covered by a cut-off filter of 420 nm with a power of 300 W and an intensity of 0.96 W/cm2. The catalytic bed was composed of 1.2 g of photocatalyst mixed with 20 g glass spheres in order to improve the fluidization property. The reactor inlet reactants and outlet products were analyzed using gas chromatography (Agilent GC 7890A model). The reactor was irradiated after complete adsorption of cyclohexane on the catalyst surface. The photocatalytic behavior of all analyzed samples was evaluated as:
X=(C0−Ct)/C0×100%where X=cyclohexane conversion, C0=inlet cyclohexane concentration, and Ct=outlet cyclohexane concentration.
5
Photocatalytic Oxidation of Cyclohexane
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Example 4
Photocatalytic Oxidation of Cyclohexane: Experimental
Photocatalytic experiments were performed by feeding a N2 stream at 30 L/h (STP) containing 200 ppm cyclohexane, 10 vol. % O2 at a temperature of 60° C. and a reaction pressure of 1 atm. Nitrogen functioned as the carrier gas for cyclohexane. Additionally, 320 ppm of water vaporized from 60° C. controlled saturators was added in order to minimize photodeactivation of the catalyst. A fluidized bed photoreactor was used as the reactor, which was irradiated by a Xenon lamp covered by a cut-off filter of 420 nm with a power of 300 W and an intensity of 0.96 W/cm2. The catalytic bed was composed of 1.2 g of photocatalyst mixed with 20 g glass spheres in order to improve the fluidization property. The reactor inlet reactants and outlet products were analyzed using gas chromatography (Agilent GC 7890A model). The reactor was irradiated after complete adsorption of cyclohexane on the catalyst surface. The photocatalytic behavior of all analyzed samples was evaluated as:
X=(C0C1)/C0×100%where X=cyclohexane conversion, C0=inlet cyclohexane concentration, and C1=outlet cyclohexane concentration.
6
Analytical Techniques for Chlorethenes Quantification
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Analyses were performed as described in Ref. [17 (link)]. Chlorethenes were measured by headspace gas chromatography (GC) coupled with flame ionization (FID) and electron single detector (ECD). The model 7890A GC from Agilent Technologies (Waldbronn, Germany) with the autosampler G1888 was used. Duplicate measurements were performed. Chloride was determined with the 761 Compact IC ion chromatograph from Metrohm (Filderstadt, Germany) with a conductivity detector and a MetrosepA-Supp-5 column. DOC was analyzed with the Vario TOC Tube from Elementar Analysesysteme GmbH (Hanau, Germany). During sampling of the microcosms, the oxygen content, as well as the pH value, were measured with the Multi 350i and pH320 multimeters and the corresponding CellOx 325 and SenTix 41 electrodes from WTW (Weilheim, Germany).
7
GC Analysis of Organic Compounds
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The instrument used was the model 7890A GC of the Agilent Company (Santa Clara, CA, USA), and it was matched with the company’s DB-1 (60 m × 0.25 mm × 0.25 mm) separation column; the heating condition was an initial temperature of 40 °C, maintained for 1 min, then raised to 150 °C at 5 °C/min, maintained for 1 min, and finally raised to 200 °C at 10 °C/min, which was maintained for 11 min. The detector used was the Flame Ionization Detector (FID), the injection mode was the splitless mode, the temperature of the injection port was 250 °C, and the temperature of the detector was 300 °C. The carrier gas used was nitrogen, and the gas flow rate was 1.0 mL/min.
8
Gas Chromatography Analysis Protocol
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GC was performed with an Agilent Model 7890A GC (Santa Clara, CA, USA), with a 60 m × 0.25 mm id Agilent DB-1 fused-silica capillary non-polar column with a film thickness of 0.25 μm; the HS-SPME injection mode was splitless, and the injection mode of direct injection was split. The GC heating conditions were as follows: the initial temperature was maintained at 40 °C for 1 min, then raised to 150 °C at 5 °C/min, maintained for 1 min, then raised to 200 °C at 10 °C/min and maintained for 11 min. The inlet temperature was 250 °C, the detector temperature was 300 °C, and a flame ionization detector (FID) was used for detection. The carrier gas was nitrogen at a flow rate of 1 mL/min.
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