For extraction of DNA from venous blood, Qiagen DSP DNA kits were used with the Qiasymphony liquid handling platform (Qiagen, Manchester, UK). For extraction of DNA from DBS, the United States Centers for Disease Control and Prevention (CDC) DBS DNA extraction method [43 ] was modified and automated using a custom program on the Biomek FXp liquid handling platform (Beckmann Coulter, Brea, CA, USA) (Figure S2 ). Single 6 mm DBS punches in a 0.8 mL 96 well plate covered with a pierceable adhesive plastic seal were used as the input material. Following the addition of the final 60 uL of Qiagen Elution Solution, plates were covered with a non-pierceable adhesive plastic seal and incubated at 99 °C for 15 min. The eluate was then transferred into a new 96 well plate using the Biomek FXp liquid handling platform.
Biomek fxp liquid handling platform
Manufactured by Beckman Coulter
Sourced in United States
The Biomek FXp liquid handling platform is a programmable automated pipetting system designed for a variety of laboratory applications. It offers precise and accurate liquid handling capabilities to streamline sample preparation and liquid transfer workflows.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using biomek fxp liquid handling platform
1
Automated DNA Extraction from Blood and DBS
2
Automated DNA Extraction from Venous Blood and DBS
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For extraction of DNA from venous blood, Qiagen DSP DNA kits were used with the Qiasymphony liquid handling platform (Qiagen, Manchester, UK). For extraction of DNA from DBS, the United States Centers for Disease Control and Prevention (CDC) DBS DNA extraction method [43 ] was modified and automated using a custom program on the Biomek FXp liquid handling platform (Beckmann Coulter, Brea, CA, USA) (Figure S2 ). Single 6 mm DBS punches in a 0.8 mL 96 well plate covered with a pierceable adhesive plastic seal were used as the input material. Following the addition of the final 60 uL of Qiagen Elution Solution, plates were covered with a non-pierceable adhesive plastic seal and incubated at 99 °C for 15 min. The eluate was then transferred into a new 96 well plate using the Biomek FXp liquid handling platform.
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