Isolated EV (2.5 μL) were dried onto freshly glow-discharged 300 mesh Formvar/carbon-coated TEM grids (Ted Pella, Redding, CA, USA) and negatively stained with 2% aqueous uracyl acetate. EV samples were visualised by TEM using Hitachi H7600 (Hitachi High-Technologies Corp., Tokyo, Japan) operated at 80 kV. Images were captured with a side mounted 1 K AMT Advantage digital camera (Advanced Microscopy Techniques, Corp. Woburn, MA, USA).
Formvar carbon coated tem grid
Manufactured by Ted Pella
Sourced in United States
Formvar/carbon-coated TEM grids are a type of specimen support used in transmission electron microscopy (TEM). These grids consist of a thin film of formvar, a synthetic polymer, coated with a thin layer of carbon. The formvar provides a stable support structure, while the carbon coating enhances the contrast and durability of the sample during TEM observation.
Automatically generated - may contain errors
Lab products found in correlation
Jem 1100, by JEOL (2 mentions)
H 7600, by Hitachi (1 mentions)
1k amt advantage digital camera, by Advanced Microscopy Techniques (1 mentions)
1200ex microscope, by JEOL (1 mentions)
H 7500 transmission electron microscope, by Hitachi (1 mentions)
Osmium tetroxide, by Merck Group (1 mentions)
Ht7700, by Hitachi (1 mentions)
6 protocols using formvar carbon coated tem grid
1
Negative Staining of Extracellular Vesicles
2
Imaging and Visualization of Gold Nanoparticles
3
Negative Staining of Extracellular Vesicles
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A 1-µl sample of purified EVs was mixed with 100 µl PBS solution, dried on a newly discharged 300-mesh Formvar/carbon-coated TEM grid (Ted Pella, Inc.) at room temperature, then negatively stained with 2% potassium phosphotungstate overnight at room temperature. All transmission electron micrographs were obtained using an H7500 transmission electron microscope (Hitachi, Ltd.) operating at 80 kV.
4
Exosome Characterization by TEM
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The isolated exosomes (10 mg) in PBS were placed on a 200 mesh Formvar/Carbon-coated TEM grid (Ted Pella, Inc, Redding, CA, USA) for 30 min. The grid with the absorbed exosomes was fixed with 2.5% glutaraldehyde in 4% paraformaldehyde for 10 min. After washing with PBS, the grid was incubated with 1% osmium tetroxide (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 4˚C and then dehydrated with 99% ethanol for 20 min. The grid was air-dried before being examined under the electron microscope (Hitachi HT7700).
5
Visualizing Particle Trimer Nanostructures
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TEM was used to control the correct assembly of the particle trimer nanostructures. A droplet of the solution containing the purified structures was deposited on a plasma-exposed carbon-formvar-coated TEM grid (Ted Pella) and then dabbed off after 3 minutes. The grid was stained with 1% uranyl formate for 15 seconds. Imaging was performed with a JEOL JEM-1100 at an acceleration voltage of 80 kV.
6
Visualizing Particle Trimer Nanostructures
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