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Formvar carbon coated tem grid

Manufactured by Ted Pella
Sourced in United States

Formvar/carbon-coated TEM grids are a type of specimen support used in transmission electron microscopy (TEM). These grids consist of a thin film of formvar, a synthetic polymer, coated with a thin layer of carbon. The formvar provides a stable support structure, while the carbon coating enhances the contrast and durability of the sample during TEM observation.

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6 protocols using formvar carbon coated tem grid

1

Negative Staining of Extracellular Vesicles

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Isolated EV (2.5 μL) were dried onto freshly glow-discharged 300 mesh Formvar/carbon-coated TEM grids (Ted Pella, Redding, CA, USA) and negatively stained with 2% aqueous uracyl acetate. EV samples were visualised by TEM using Hitachi H7600 (Hitachi High-Technologies Corp., Tokyo, Japan) operated at 80 kV. Images were captured with a side mounted 1 K AMT Advantage digital camera (Advanced Microscopy Techniques, Corp. Woburn, MA, USA).
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2

Imaging and Visualization of Gold Nanoparticles

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The structures of the AuNPs and oligonucleotide-functionalized AuNPs were examined using a JEOL 1200EX microscope, and the interactions of AuNPs with plant cells were studied with a Tecnai 12 transmission electron microscope (Berkeley Electron Microscope Lab). AuNPs were dropcast on plasma-treated Formvar carbon-coated TEM grids (Ted-Pella), then imaged at an acceleration voltage of 120 kV. To visualize siRNA on NA-AuNPs, TEM grids with dropcast NA-AuNPs were placed face down on a 2% methanolic uranyl acetate droplet for 30 s, removed and blotted with filter paper, and air-dried prior to imaging.
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3

Negative Staining of Extracellular Vesicles

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A 1-µl sample of purified EVs was mixed with 100 µl PBS solution, dried on a newly discharged 300-mesh Formvar/carbon-coated TEM grid (Ted Pella, Inc.) at room temperature, then negatively stained with 2% potassium phosphotungstate overnight at room temperature. All transmission electron micrographs were obtained using an H7500 transmission electron microscope (Hitachi, Ltd.) operating at 80 kV.
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4

Exosome Characterization by TEM

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The isolated exosomes (10 mg) in PBS were placed on a 200 mesh Formvar/Carbon-coated TEM grid (Ted Pella, Inc, Redding, CA, USA) for 30 min. The grid with the absorbed exosomes was fixed with 2.5% glutaraldehyde in 4% paraformaldehyde for 10 min. After washing with PBS, the grid was incubated with 1% osmium tetroxide (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 4˚C and then dehydrated with 99% ethanol for 20 min. The grid was air-dried before being examined under the electron microscope (Hitachi HT7700).
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5

Visualizing Particle Trimer Nanostructures

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TEM was used to control the correct assembly of the particle trimer nanostructures. A droplet of the solution containing the purified structures was deposited on a plasma-exposed carbon-formvar-coated TEM grid (Ted Pella) and then dabbed off after 3 minutes. The grid was stained with 1% uranyl formate for 15 seconds. Imaging was performed with a JEOL JEM-1100 at an acceleration voltage of 80 kV.
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6

Visualizing Particle Trimer Nanostructures

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TEM was used to control the correct assembly of the particle trimer nanostructures. A droplet of the solution containing the purified structures was deposited on a plasma-exposed carbon-formvar-coated TEM grid (Ted Pella) and then dabbed off after 3 minutes. The grid was stained with 1% uranyl formate for 15 seconds. Imaging was performed with a JEOL JEM-1100 at an acceleration voltage of 80 kV.
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