Anti shank3

Standard immunocytochemistry and Western blot techniques were used to characterize cell type–specific markers and phenotypically relevant protein levels. The following antibodies were used for immunostaining according to the manufacturers’ instructions: anti-GFP (#1010; Aves Labs Inc), anti-PAX6 (PRB-278P; Covance), anti-Nestin (PRB-315C; Covance), anti-Map2 (#M2320; Sigma-Aldrich), and anti-Ctip2 (ab18465; Abcam). The following antibodies were used for Western blot assays: anti-Shank3 (sc30193; Santa Cruz), anti-Shank3 (sc 23547; Santa Cruz), anti-PSD95 (ab2723; Abcam), anti-Homer (#160011; Synaptic Systems), anti-NLGN1 (SAB1407153; Sigma-Aldrich), anti-PIKE (#07-675; Upstate), anti-Akt (#4691S; Cell Signaling), anti-phospho Akt (#4058; Cell Signaling), anti-mTOR (#4691; Cell Signaling), anti-phospho mTOR (#2971; Cell Signaling), anti-Jip2 (N135/37; NeuroMab), anti-NRP1 (SAB1411572; Sigma-Aldrich), anti-JNK1/3 (sc474; Santa Cruz), anti-phosphor JNK (#V793A; Promega), anti-DCX (#4604; Cell Signaling), anti-NeuN (#MAB377; EMD Millipore), anti-Tuj1 (#903401; BioLegend), anti-GAPDH (sc 47724; Santa Cruz), and anti-Actin (sc1616; Santa Cruz). Western blot quantification was performed using ImageJ.

The following primary antibodies were used: anti-Shank1 (1:200, Abcam, catalog #ab154224), anti-Shank2 (1:100, Cell Signaling, catalog #12218), anti-Shank3 (1:400, Santa Cruz, RRID: AB_2301759), anti-PanShank (1:1000, Neuromab, RRID: AB_10674115), and anti-actin (1:3000, Sigma, RRID: AB_476697). IRDye 800CW and 680LT Secondary antibodies (Licor) were used at 1:5000 dilution for detection on an Odyssey IR laser Scanner (Licor). All anti-Shank signals were normalized to the actin signal. Data from infected neurons were compared to data from uninfected neurons within the same batch. Statistical significance was estimated with Student’s t test between infected and uninfected neuron cultures.