F10prdmyr 1

Manufactured by Olympus

The F10PRDMYR-1 is a high-precision laboratory instrument designed for accurate measurement and analysis. It features advanced sensor technology and robust construction for reliable performance in various research and testing applications. The core function of this product is to provide precise and consistent data acquisition capabilities for scientific investigations.

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Lab products found in correlation

P35gc 1.5 14 c, by MatTek (2 mentions) Fv1000 confocal microscopy system, by Olympus (2 mentions)

2 protocols using f10prdmyr 1

Laser-Induced Stress Response Kinetics

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The Olympus FV1000 confocal microscopy system (Cat. F10PRDMYR-1, Olympus) and FV1000 software were used for the acquisition of images. Cells were cultured in 35 mm glass-bottom dishes (MatTek, P35GC-1.5-14-C) before observation. The damage was induced with a 405 nm laser. The laser passed through a PLAON 60X oil lens. Cells transfected with GFP-tagged proteins were incubated at 37 °C on a thermos plate in normal media during observation. For the evaluation of accumulation and kinetics, the mean intensity of each accumulated point or line was obtained after subtraction and quantified by ImageJ.

Wang Y., Gao B., Zhang L., Wang X., Zhu X., Yang H., Zhang F., Zhu X., Zhou B., Yao S., Nagayama A., Lee S., Ouyang J., Koh S.B., Eisenhauer E.L., Zarrella D., Lu K., Rueda B.R., Zou L., Su X.A., Yeku O., Ellisen L.W., Wang X.S, & Lan L. (2024). Meiotic protein SYCP2 confers resistance to DNA-damaging agents through R-loop-mediated DNA repair. Nature Communications, 15, 1568.

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Confocal Imaging of KR Activation

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The Olympus FV1000 confocal microscopy system (Cat. F10PRDMYR-1, Olympus) and FV1000 software were used for the acquisition of images. Cells were cultured in 35 mm glass-bottom dishes (MatTek, P35GC-1.5–14-C) before observation. Activation of KR in bulky cells was completed by exposing cells to a 15 W Sylvania cool white fluorescent bulb for 25 min in a stage UVP (Uvland, CA). The intensity was measured by ImageJ 1.52i software.

Zhu X., Wang X., Yan W., Yang H., Xiang Y., Lv F., Shi Y., Li H.Y, & Lan L. (2021). Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response. NAR Cancer, 3(1), zcab010.

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