Immunohistochemistry staining of F4/80, a marker of macrophages, was performed on 4 µm paraffin sections treated by 40 µg/ml proteinase K (Sigma, Dorset, UK) at 37°C, 30 min for antigen retrieving. F4/80 (1:50, ab11101, Abcam, Cambridge, UK) was incubated at 4°C overnight. DAKO EnVision™ + Dual Link System-HRP was used as the secondary antibody (DAKO, Glostrup, Denmark). The antibody binding was revealed by 3,3’-diaminobenzidine (SK-4100, DAB, Vector, Burlingame, USA) with hematoxylin counterstaining. The number of macrophages in the tubular, interstitial, and tubular lumen areas were semi-quantitatively analysed in 20 fields at 400× magnification.
Ab11101
Manufactured by Abcam
Sourced in United Kingdom
Ab11101 is a lab equipment product manufactured by Abcam. It is a general-purpose tool used in scientific research and laboratory environments.
Automatically generated - may contain errors
2 protocols using ab11101
1
Macrophage Quantification in Kidney Tissue
2
Histological Analysis of Liver Inflammation
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Liver tissue sections (4- m-thick) were stained using haematoxylin and eosin (H&E) to observe inflammation and tissue damage using light microscopy. F4/80 and STING were identified by immunofluorescence using a primary rat anti-mouse F4/80 (1:100, ab11101, Abcam) and STING (1:200, #13647S, Cell Signaling Technology) antibodies, and secondary biotinylated goat anti-rat IgG (1:100, BA-1000, Vector) according to the manufacturer's instructions. Positive cells were counted blindly at 10 HPE/section.
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