MACS sorting of hCD2 (+) cells was performed using CD2 Microbeads (130-091-114, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany). To monitor the results of MACS sorting, cells were stained with phycoerythrin (PE)-conjugated anti-hCD2 Ab (347597, BD Life Sciences, San Jose, CA, USA) for 30 min at 4°C. Flow cytometry was performed on a FACSCalibur (BD Life Sciences), and data were analyzed using the FlowJo software (BD Life Sciences).
Pe conjugated anti hcd2 ab
Manufactured by BD
Sourced in United States
The PE)-conjugated anti-hCD2 Ab is a laboratory reagent that binds to the human CD2 antigen. It is typically used in flow cytometry applications to identify and analyze CD2-positive cells.
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Lab products found in correlation
3 protocols using pe conjugated anti hcd2 ab
1
MACS Sorting of hCD2-Positive Cells
2
MACS Sorting of Human CD2+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MACS sorting of human CD2 (hCD2) (+) cells was performed using CD2 Microbeads (130-091-114, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany). To monitor the results of MACS sorting, cells were stained with phycoerythrin (PE)-conjugated anti-hCD2 Ab (347597, BD Life Sciences, San Jose, CA, USA) for 30 min at 4°C. Flow cytometry was performed on a FACSCalibur (BD Life Sciences), and data were analyzed using the FlowJo software (BD Life Sciences). enChIP-real-time PCR enChIP-real-time PCR was performed as previously described (13) . The primers used in this study were reported previously (1) .
3
MACS Sorting of Human CD2+ Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MACS sorting of human CD2 (hCD2) (+) cells was performed using CD2 Microbeads (130-091-114, Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany). To monitor the results of MACS sorting, cells were stained with phycoerythrin (PE)-conjugated anti-hCD2 Ab (347597, BD Life Sciences, San Jose, CA, USA) for 30 min at 4°C. Flow cytometry was performed on a FACSCalibur (BD Life Sciences), and data were analyzed using the FlowJo software (BD Life Sciences). enChIP-real-time PCR enChIP-real-time PCR was performed as previously described (13) . The primers used in this study were reported previously (1) .
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