Schizophyllum commune was grown from a small agar inoculum on solid Schizophyllum commune minimal medium (SCMM) supplemented with 1.5% agar at 30 °C45 (link). All strains are derived from S. commune H4-8 (matA43matB41; FGSC 9210) and the compatible isogenic strain H4-8b (matA41matB43)32 (link),36 (link). For deletions, a previously published Δku80 strain was used31 (link). For selection on nourseothricin (Bio-Connect, Netherlands) or phleomycin (Bio-Connect, Netherlands), SCMM was supplemented with 15 μg/mL and 25 μg/mL antibiotic, respectively46 (link). For phenotypical characterization, strains were grown for 7 days at 25 °C in a 16/8 h day/night cycle.
Nourseothricin
Manufactured by Bio-Connect
Sourced in Netherlands
Nourseothricin is a selective antibiotic used in molecular biology applications. It functions as a resistance marker for genetic engineering and cell line selection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nourseothricin
1
Cultivation of Schizophyllum commune Fungus
2
Culturing Schizophyllum commune Fungus
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S. commune was grown from a small agar inoculum in Schizophyllum commune minimal medium (SCMM) at 30 °C48 (link). For solid culture, the medium was supplemented with 1.5% agar. All strains are derived from S. commune H4-8 (matA43matB41; FGSC 9210)5 (link),10 (link). Strain Δku8015 and strain Δhom26 (link) were published previously. For selection on nourseothricin (Bio-Connect, Netherlands) or phleomycin (Bio-Connect, Netherlands), SCMM was supplemented with 15 μg ml−1 and 25 μg ml−1 antibiotic, respectively40 (link). For phenotypical characterization, strains were grown for 7 days at 25 °C in a 16/8 hours day/night cycle.
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