In this study, the following instruments were used: Gas Chromatography Mass Spectrometry (GC-MS) (QP-5000 GC-MS Shimadzu, Japan), ultrasonic-microwave cooperative extractor/reactor (CW-2000, China), rotary evaporator (Heidolph OB2000, VV2000, Germany), UV visible spectrophotometer (Jenway 7315, England), grinder (Moulinex model, Uno. China), balance (Radw ag, AS 220/c/2, Poland), filter paper (Machrery-Nagel, MN 617 and Whatman no.1, USA), micropipettes (Finnpipette, Finland), incubator (Nuve, Turkey), syringe filter 0.45 μm pore size (Microlab, China) and 96-well plates (Greiner bio-one, North America).
Qp 5000 gc ms
Manufactured by Shimadzu
Sourced in Japan
The QP-5000 GC-MS is a gas chromatography-mass spectrometry (GC-MS) instrument manufactured by Shimadzu. It is designed to perform qualitative and quantitative analysis of complex organic and inorganic compounds by combining gas chromatography separation and mass spectrometry detection.
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4 protocols using qp 5000 gc ms
1
Comprehensive Phytochemical Analysis
2
GC-MS Analysis of Compounds
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GC-MS chromatograms were recorded using Shimadzu QP-5000 GC-MS. The GC was equipped with Rtx-5 ms column (30 m long, 0.25 μm thickness and 0.250 mm inner diameter). Helium was used as a carrier gas at a flow rate of 1 ml/min. Injector temperature was 220 °C. Oven temperature was programmed from 50 °C (1 min hold) at 5 °C/min to 130 °C, then at 10 °C/min to 250 °C and kept isothermally for 15 min. Transfer line temperature was 290 °C. For GC-MS detection, an electron ionization system, with detector volts of 1.7 KV was used. A scan rate of 0.5 s, and scan speed 1000 amu/s was applied, covering a mass range from 38–450 M/Z.
3
GC-MS Analysis of Metabolites
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The GC-MS chromatograms were recorded using Shimadzu QP-5000 GC-MS. The GC was equipped with Rtx-5 ms column (30 m long, 0.25 μm thickness, and 0.25 mm inner diameter). Helium was used as a carrier gas at a flow rate of 1 ml/min. The injector temperature was 220°C. The oven temperature was programmed from 50°C (1 min hold) at 5°C/min to 130°C and then at 10°C/min to 250°C and was kept isothermally for 15 min. Transfer line temperature was 290°C.
For GC-MS detection, an electron ionization system, with detector volts of 1.7 KV, was used. A scan rate of 0.5 s and scan speed 1000 amu/sec were applied, covering a mass range 38–450 M/Z.
For GC-MS detection, an electron ionization system, with detector volts of 1.7 KV, was used. A scan rate of 0.5 s and scan speed 1000 amu/sec were applied, covering a mass range 38–450 M/Z.
4
GC-MS Analysis of Essential Oils
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Gas chromatography mass spectrometry (GC-MS) (QP-5000 GC-MS Shimadzu, Japan) was used to assess the chemical composition of the essential oils. Ultrasonic-microwave cooperative extractor/reactor (CW-2000, China) was used to extract the essential oils. UV-visible spectrophotometer (Jenway 7315, England) was used to assess the antioxidant activity. Grinder (Moulinex model, Uno, China) was used to mill the dried plants. Balance (Radw ag, AS 220/c/2, Poland) was used for sensitive weighing of the samples. Filter papers (Macherey-Nagel, MN 617 and Whatman no. 1, USA), micropipettes (Finnpipette, Finland), incubator (Nuve, Turkey), syringe filter 0.45 μm pore size (Microlab, China), and 96-well plates (Greiner bio-one, North America) were used for antimicrobial tests.
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