Mouse monoclonal anti erk

The following primary antibodies were used in this study: Mouse monoclonal anti-RHOG, mouse monoclonal anti-RHOA, rabbit polyclonal anti-RHOC, mouse monoclonal anti-RAC1, rabbit monoclonal anti-ROCK1, rabbit monoclonal anti-ROCK2, rabbit polyclonal anti-p-AKT1 (phospho-S473), rabbit polyclonal anti-pan-AKT, and rabbit polyclonal anti-Actin antibodies, as well as the MEK1/2 inhibitor U0126 and the PI3K inhibitor Wortmannin, which were obtained from Abcam (Abcam Inc., Cambridge, UK). Rabbit polyclonal anti-CDC42 (sc-87), mouse monoclonal anti-p-ERK1 (phospho-Y204), mouse monoclonal anti-ERK, and goat polyclonal anti-STARD13 antibodies were obtained from Santa Cruz (Santa Cruz Biotechnology., Santa Cruz, CA, US). Anti-rabbit and anti-mouse HRP-conjugated secondary antibodies were obtained from Promega (Promega Co., Maddison, WI, USA). The Dominant active Cherry-tagged RAC1-Q61L and Cherry-tagged CDC42-Q61L constructs were a generous gift from Dr. Louis Hodgson.

Goat polyclonal anti-COX-2 (human), rabbit polyclonal anti-pERK and mouse monoclonal anti-ERK were obtained from Santa Cruz Biotechnologies (Santa Cruz, CA). Rabbit polyclonal anti-EP₁, EP₂, EP₃ and EP₄ receptors (human) were from Cayman Chemical (Ann Arbor, MI) as was arachidonic acid. 17-phenyl-trinor prostaglandin E₂ (17E₂) was from Biomol -Enzo Life Sciences (Pharmingdale, NY). Horseradish peroxidase-conjugated bovine anti-goat IgG, goat anti-rabbit IgG, and goat anti-mouse IgG were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA). All other reagents were standard laboratory grade.