For protein subcellular localization, transiently expressing PtaHMGB2/3:YFP and YFP N. benthamiana leaves were sectioned and observed using Zeiss Axiophot epifluorescence microscope. Images were captured with 40× magnification by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition.
For the quantification of YFP, HMGB2/3:YFP and HMGB6:YFP fluorescence, discs images were captured by a Leica MZ10F fluorescence binocular loupe, using 10× magnification. Images were recorded by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition. Relative fluorescence (fold-increase) was calculated using Fiji software [77 (link)] as previously described [78 (link)].
For the quantification of YFP, HMGB2/3:YFP and HMGB6:YFP fluorescence, discs images were captured by a Leica MZ10F fluorescence binocular loupe, using 10× magnification. Images were recorded by a Leica DFC 300FX CCD color camera equipped with Leica Application Suite 2.8.1 build 1554 acquisition. Relative fluorescence (fold-increase) was calculated using Fiji software [77 (link)] as previously described [78 (link)].