Anti hydroxy hif1α pro564

Manufactured by Cell Signaling Technology

Anti-hydroxy-HIF1α (Pro564) is a laboratory antibody that recognizes the hydroxylated form of the hypoxia-inducible factor 1-alpha (HIF1α) protein at the proline 564 residue. This antibody can be used to detect and study the regulation of HIF1α, a transcription factor that plays a central role in the cellular response to hypoxic conditions.

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Lab products found in correlation

Anti gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions) Anti myc sc 40, by Santa Cruz Biotechnology (1 mentions) Normal rabbit immunoglobulin g, by Cell Signaling Technology (1 mentions) Anti egln1, by Cell Signaling Technology (1 mentions) Anti set7, by Cell Signaling Technology (1 mentions) Anti set7, by Abcam (1 mentions) Anti hif 1α, by Cell Signaling Technology (1 mentions) Mg132, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Anti ha, by Fortrea (1 mentions) Anti β actin, by ABclonal (1 mentions) Anti vimentin, by Proteintech (1 mentions) Ecl plus system, by GE Healthcare (1 mentions) Anti rabbit or anti mouse secondary antibodies, by GE Healthcare (1 mentions) Anti hif 1α, by Proteintech (1 mentions) Anti e cadherin, by Abcam (1 mentions)

2 protocols using anti hydroxy hif1α pro564

Antibody Procurement and Utilization

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Antibodies including anti-EGLN1 (#4835), anti-HIF1α (#36169), anti-hydroxy-HIF1α (Pro564) (#3434), anti-SET7 (#2825), and normal rabbit immunoglobulin G (#2729) were purchased from Cell Signaling Technology. Anti-β-actin (#AC026) was purchased from ABclonal. Anti-Flag (#F1804) was purchased from Sigma. Anti-HA (#901515) was purchased from Covance. Anti-Myc (#SC-40) and Anti-GAPDH (#SC-47724) antibody was purchased from Santa Cruz Biotechnology. anti-SET7 (#ab124708) was purchased from Abcam. MG-132 (#474790) was purchased from Sigma. Cycloheximide (#HY-12320) was purchased from MCE.

Tang J., Deng H., Wang Z., Zha H., Liao Q., Zhu C., Chen X., Sun X., Jia S., Ouyang G., Liu X, & Xiao W. (2022). EGLN1 prolyl hydroxylation of hypoxia-induced transcription factor HIF1α is repressed by SET7-catalyzed lysine methylation. The Journal of Biological Chemistry, 298(6), 101961.

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Protein Expression Profiling in Cellular Lysates

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Cells were lysed in an SDS lysis buffer for 30 min before heating for 10 min at 100°C. Proteins were probed with a range of primary antibodies: anti‐OGDHL (Proteintech), anti‐HIF‐1α, anti‐DLD (Proteintech), anti‐DLST (Abcam), anti‐E‐cadherin (Abcam); anti‐vimentin (Proteintech); anti‐hydroxy‐HIF‐1α (Pro564; Cell Signaling); and anti‐PHD2 and anti‐β‐actin (Proteintech). Primary antibodies were examined through anti‐mouse or anti‐rabbit secondary antibodies (GE Healthcare Bioscience) and developed by the ECL‐PLUS system (GE Healthcare Bioscience).

Dai W., Li Y., Sun W., Ji M., Bao R., Chen J., Xu S., Dai Y., Chen Y., Liu W., Ge C., Sun W., Mo W., Guo C, & Xu X. (2023). Silencing of OGDHL promotes liver cancer metastasis by enhancing hypoxia inducible factor 1 α protein stability. Cancer Science, 114(4), 1309-1323.

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