Log In Sign up
The largest database of trusted experimental protocols

Asipro software

Manufactured by Siemens
Visit Supplier
Sourced in Germany

ASIPro software is a tool for the analysis and optimization of industrial automation systems. It provides a platform for the configuration, programming, and monitoring of Siemens automation devices and components.

Automatically generated - may contain errors

Lab products found in correlation

Inveon, by Siemens (2 mentions) Concorde microsystems focus 220, by Siemens (2 mentions) Inveon pet scanner, by Siemens (1 mentions) Focus f 120, by Siemens (1 mentions) Micropet manager, by Siemens (1 mentions) Biopet, by Bioscan (1 mentions) Asipro vm micro pet analysis software, by Siemens (1 mentions) Micropet r4 rodent scanner, by Siemens (1 mentions)

Close spelling variants of this product

ASIPro VW software ASIPro VM™ Micro PET Analysis software ASIPro

12 protocols using asipro software

1

PET Imaging of Hippocampal Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
18F-FDG (1 mCi) was injected via the tail vein, and the injected mice were anesthetized with 2% isoflurane in 100% oxygen. A Siemens Inveon PET scanner (Siemens Medical Solutions, Malvern, PA, USA) was used for PET imaging. The transverse resolution was <1.8 mm at the center. After allowing uptake for 10 min, 30 min of emission PET data were acquired with a 350-650 keV energy window. The list-mode PET data were reconstructed using 3D reprojection methods. The pixel size of the reconstructed images was 0.15 × 0.15 × 0.79 mm3. Attenuation, scatter corrections, and normalization were performed. To identify regional differences between groups, a region of interest (ROI) was drawn in the hippocampus. The maximal value of the ROI was calculated within ROI regions to avoid a partial volume effect. ROIs were drawn on at least 10 subsequent coronal sections of PET data, and then the averaged value of the PET counts of individual mice were used for statistics. Asipro Software (Siemens Healthineers, Erlangen, Germany) was used for ROI delineation. For normalization of body weight and injected dose differences, the PET counts of the hippocampus were normalized to the whole-brain counts.
Yang H., Park S.Y., Baek H., Lee C., Chung G., Liu X., Lee J.H., Kim B., Kwon M., Choi H., Kim H.J., Kim J.Y., Kim Y., Lee Y.S., Lee G., Kim S.K., Kim J.S., Chang Y.T., Jung W.S., Kim K.H, & Bae H. (2022). Adoptive therapy with amyloid-β specific regulatory T cells alleviates Alzheimer's disease. Theranostics, 12(18), 7668-7680.
+ Open protocol
+ Expand
2

PET/CT Imaging of Tumor Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were imaged using a small animal PET/CT system (INVEON™; Siemens Preclinical Solutions, Knoxville, TN, USA). [18F] Fluordeoxyglucose (FDG) (7.4 MBq, 200 μCi) was injected via tail vein 1 h prior to PET/CT scanning. [18F] fluorothymidine (FLT) (same dose) was injected 2 h prior. Mice were anesthetized using 2% isoflurane. PET and CT images were acquired using small animal PET/CT scanner. The mice were moved to the PET scanner on the same bed and scanned for 30 min after CT acquisition. Tissue radioactivity was expressed as the percentage of injected radioactivity dose per gram of tissue (%ID/g). Visualization and analyses of PET images were carried out using AsiPRO™ software (Siemens Preclinical Solutions). Radioactivity concentration in the local region was calculated from the PET images using maximum pixel values.
JANG S.J., KANG J.H., LEE Y.J., KIM K.I., LEE T.S., CHOE J.G, & LIM S.M. (2016). Detection of metastatic tumors after γ-irradiation using longitudinal molecular imaging and gene expression profiling of metastatic tumor nodules. International Journal of Oncology, 48(4), 1361-1368.
+ Open protocol
+ Expand
3

PET Imaging of PD-L1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with isoflurane in oxygen (2% induction and maintenance); tail vein catheters were installed then transferred to the microPET F120 scanner (Siemens). A 10-min transmission image was acquired using a 57 Co point source for attenuation correction of the final PET images. Then approximately 5.6 MBq of 18 F-BMS-986192 (n 5 7) were administered via the tail vein. Two-hour dynamic emission images were acquired for all animals. For blocking studies, animals (n 5 4) received 3 mg/kg ADX_5322_A02 anti-PD-L1 adnectin coadministered with 18 F-BMS-986192. PET data were reconstructed using a 3-dimensional ordered-subsets expectation maximization followed by maximum a posteriori algorithm corrected for attenuation using the previously acquired transmission scan. Images were analyzed using ASIPro software (Siemens), with regions of interest drawn around tumors and radiotracer uptake expressed as percentage injected dose per gram (%ID/g).
Donnelly D.J., Smith R.A., Morin P., Lipovšek D., Gokemeijer J., Cohen D., Lafont V., Tran T., Cole E.L., Wright M., Kim J., Pena A., Kukral D., Dischino D.D., Chow P., Gan J., Adelakun O., Wang X.T., Cao K., Leung D., Bonacorsi SJ J.r, & Hayes W. (2018). Synthesis and Biologic Evaluation of a Novel 18F-Labeled Adnectin as a PET Radioligand for Imaging PD-L1 Expression. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 59(3).
+ Open protocol
+ Expand
4

Multimodal Imaging of Tumor Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals were imaged with 18F-FDG and 18F-FGln (24 hours apart to allow complete decay of radiotracer) by injecting 200 μCi of the radiotracer into the lateral tail vein. PET imaging was performed using a dedicated small-animal microPET scanner (Concorde Microsystems) under 2% isoflurane anesthesia, with the tumors centered in the field of view. Dynamic imaging was performed by obtaining sixty-minute acquisitions with an energy window of 350–750 keV with a coincidence-timing window of 6 ns. For static imaging, acquisitions were collected 0.5 h, 1 h and 2 h after injection. In each animal, the MRI and PET images were directly compared. Region-of-interest (ROI) analysis of the acquired images was performed using ASIPro software (Siemens) in a non-blinded manner, and the observed maximum pixel value was represented as percent-injected dose/cubic centimeter (%ID/cc). Tumor to brain ratios were determined by normalizing tumor uptake to surrounding brain uptake.
Venneti S., Dunphy M.P., Zhang H., Pitter K.L., Zanzonico P., Campos C., Carlin S.D., La Rocca G., Lyashchenko S., Ploessl K., Rohle D., Omuro A.M., Cross J.R., Brennan C.W., Weber W.A., Holland E.C., Mellinghoff I.K., Kung H.F., Lewis J.S, & Thompson C.B. (2015). Glutamine-based PET imaging facilitates enhanced metabolic evaluation of gliomas in vivo. Science translational medicine, 7(274), 274ra17.
+ Open protocol
+ Expand
5

In vivo PET Imaging of 4-[18F]FEBZA in Tumor Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo imaging of 4-[18F]FEBZA in mice bearing B16F1 (n = 2) and HT-29 (n = 1) tumor was performed using a P4 microPET™ scanner (Concorde Microsystems, Inc., Knoxville, TN). After administering ~100 μCi of 4-[18F]FEBZA via tail-vein injection, whole body PET scans were acquired in list mode. The images were reconstructed using filtered back projection with attenuation correction. Regions Of Interest (ROIs) were drawn on select organs using the summed image from the entire scan acquisition session, and the data were analyzed using ASIPro software (Siemens Preclinical Solutions, Knoxville, TN). The time activity curves (TACs) were generated from the list mode data reconstructed into multiple 5-min frames.
Garg P.K., Nazih R., Wu Y., Grinevich V.P, & Garg S. (2017). Selective targeting of melanoma using N-(2-diethylaminoethyl) 4-[18F]fluoroethoxy benzamide (4-[18F]FEBZA): a novel PET imaging probe. EJNMMI Research, 7, 61.
+ Open protocol
+ Expand
6

Quantifying Brown Adipose Tissue Glucose Uptake

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Glucose uptake into BAT was quantified by PET analysis of 18F-fluorodeoxyglucose (18F-FDG) uptake. Procurement of FDG was from PETNET Solutions, Inc. of Charlottesville, VA. The mice were fasted overnight before PET/CT studies. On the day of imaging, mice were given an intraperitoneal injection of CL316243 (1 mg/kg b. wt). Thirty minutes after injection of CL316243, 18F-FDG (100 μCi) was administered through tail vein injection under isoflurane anesthesia. Imaging was performed 90 min later under the same anesthesia while maintaining body temperature using a Focus F-120 small-animal microPET scanner (Siemens Medical Solutions, Inc.) following a previously published protocol [33 (link)]. Each PET acquisition was performed for 10 min. PET images were reconstructed from raw data with the OSEM3D/MAP algorithm (zoom factor, 2.164) using microPET Manager (version 2.4.1.1, Siemens). The reconstructed pixel size was 0.28 × 0.28 × 0.79 mm on a 128 × 128 × 95 image matrix. All PET images were normalized to decay correction but not for attenuation. Each image analysis was performed using ASIPRO software (Siemens) for presentation. The final PET images were used for quantitative estimates of the accumulation of 18F-FDG in the regions of interest (ROI) of BAT, and the maximum standardized uptake values (SUV) were calculated from the maximum pixel value in the image slice.
Senthivinayagam S., Serbulea V., Upchurch C.M., Polanowska-Grabowska R., Mendu S.K., Sahu S., Jayaguru P., Aylor K.W., Chordia M.D., Steinberg L., Oberholtzer N., Uchiyama S., Inada N., Lorenz U.M., Harris T.E., Keller S.R., Meher A.K., Kadl A., Desai B.N., Kundu B.K, & Leitinger N. (2020). Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels. Molecular Metabolism, 44, 101130.
+ Open protocol
+ Expand
7

PET Imaging of Tumor 4-[18F]Fluoroglutamine Uptake

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal handling methods for PET imaging studies were conducted as reported5 ,42 (link). Prior to imaging, animals were allowed to acclimate to facility environment for at least 1 h in a warmed chamber at 31.5 °C. Animals were administered 10.4–11.8 MBq 4-[18F]fluoroglutamine via intravenous injection and imaged using a Concorde Microsystems Focus 220 microPET scanner (Siemens Preclinical Solutions). During imaging, animals were maintained under 2% isoflurane anesthesia in oxygen at 2 L/min and kept warm for the duration of the PET scan. PET images in xenograft-bearing mice were acquired as 60-minute dynamic data sets. Imaging was initiated three hours post-treatment following vehicle or V-9302 (75 mg/kg) administration. PET data were reconstructed using a three-dimensional (3D) ordered subset expectation maximization/maximum a posteriori (OSEM3D/MAP) algorithm. The resulting three-dimensional reconstructions had an x-y voxel size of 0.474 mm and inter-slice distance of 0.796 mm. ASIPro software (Siemens Preclinical Solutions) was used to manually draw 3D regions of interest (ROIs) surrounding the entire tumor volume. 4-[18F]fluoroglutamine uptake was quantified as the percentage of the injected dose per gram of tissue (%ID/g). Significance was calculated using a t-test in Graphpad Prism. Error is reported as standard deviation (SD).
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
+ Open protocol
+ Expand
8

PET Imaging of Tumor 4-[18F]Fluoroglutamine Uptake

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal handling methods for PET imaging studies were conducted as reported5 ,42 (link). Prior to imaging, animals were allowed to acclimate to facility environment for at least 1 h in a warmed chamber at 31.5 °C. Animals were administered 10.4–11.8 MBq 4-[18F]fluoroglutamine via intravenous injection and imaged using a Concorde Microsystems Focus 220 microPET scanner (Siemens Preclinical Solutions). During imaging, animals were maintained under 2% isoflurane anesthesia in oxygen at 2 L/min and kept warm for the duration of the PET scan. PET images in xenograft-bearing mice were acquired as 60-minute dynamic data sets. Imaging was initiated three hours post-treatment following vehicle or V-9302 (75 mg/kg) administration. PET data were reconstructed using a three-dimensional (3D) ordered subset expectation maximization/maximum a posteriori (OSEM3D/MAP) algorithm. The resulting three-dimensional reconstructions had an x-y voxel size of 0.474 mm and inter-slice distance of 0.796 mm. ASIPro software (Siemens Preclinical Solutions) was used to manually draw 3D regions of interest (ROIs) surrounding the entire tumor volume. 4-[18F]fluoroglutamine uptake was quantified as the percentage of the injected dose per gram of tissue (%ID/g). Significance was calculated using a t-test in Graphpad Prism. Error is reported as standard deviation (SD).
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
+ Open protocol
+ Expand
9

PSMA PET Imaging of Prostate Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to imaging, all mice were anesthetized using isoflurane/O2 (1.5 % to 3 % v/v) and imaged (BioPET, Bioscan) at 1 h post [18F]DCFPyL injection (100–150 μCi; iv). Whole body images were acquired (2 bed positions; 5 min each) and reconstructed using three-dimensional ordered-subset expectation maximums (3D-OSEM) [27 (link)]. Mice in both the groups were imaged before initiation of ADT treatment (baseline) and at days 2, 7, 14, and 21 following treatment. Tumors were measured weekly (concurrent with PET imaging) by caliper, and the volume was calculated using the ellipsoid formula.
PET images were analyzed (ASIPro software, Siemens) by drawing regions of interest (ROI’s) for LuCaP73, LuCaP136, and LuCaP167 which encompassed the whole tumor volume. Additionally, ROIs were drawn for the thigh muscle, from which tumor:muscle PSMA PET uptake ratios (T:M) were calculated for each mouse. Statistical significance (P < 0.05) between the control and treated groups was determined using the student t-test.
Roy J., White M.E., Basuli F., Opina A.C., Wong K., Riba M., Ton A.T., Zhang X., Jansson K.H., Edmondson E., Butcher D., Lin F.I., Choyke P.L., Kelly K, & Jagoda E.M. (2021). Monitoring PSMA Responses to ADT in Prostate Cancer Patient-Derived Xenograft Mouse Models Using [18F]DCFPyL PET Imaging. Molecular imaging and biology, 23(5), 745-755.
+ Open protocol
+ Expand
10

Zirconium-89 Labeled Antibody Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three mice bearing both HER3/RH7777 and RH7777 tumors were injected with approximately 3.7 MBq of [89Zr]Mab#58 (approximately 60 μg protein) via the tail vein. At 1, 2, 4, and 6 days post-injection, PET data acquisition was conducted for 10–20 min by using a small-animal PET system (Inveon, Siemens Medical Solutions, Malvern, PA) under isoflurane anesthesia during the entire scanning period. Images were reconstructed using a 3D maximum a posteriori (MAP) method (18 iterations with 16 subsets, β = 0.2) without attenuation correction. Image analysis was performed using the ASIPro VM Micro PET Analysis software (Siemens, Knoxville, TN). A lamp and heating pad were used during the PET scan to maintain the body temperature of the mice. Tracer uptake was expressed as % ID/g. The region of interest was manually drawn over tumors and tracer uptake was quantified using ASI Pro software (Siemens Medical Solutions). No correction for partial volume effects was performed.
Three mice bearing the CTOS C45 xenograft were injected with 3.7 MBq of [89Zr]Mab#58 or 89Zr labeled control antibody ([89Zr]IgG), and PET data were acquired as mentioned above.
Yuan Q., Furukawa T., Tashiro T., Okita K., Jin Z.H., Aung W., Sugyo A., Nagatsu K., Endo H., Tsuji A.B., Zhang M.R., Masuko T., Inoue M., Fujibayashi Y, & Saga T. (2015). Immuno-PET Imaging of HER3 in a Model in which HER3 Signaling Plays a Critical Role. PLoS ONE, 10(11), e0143076.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!