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Anti-GFP is a monoclonal antibody that specifically binds to the green fluorescent protein (GFP). It is a widely used tool in molecular and cell biology research, allowing for the detection and visualization of GFP-tagged proteins in various experimental setups.

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Lab products found in correlation

Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Lsm 700, by Zeiss (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Anti rab11, by Abcam (1 mentions) Anti rab5, by Abcam (1 mentions) Anti rab11, by BD (1 mentions) Lamp1, by Abcam (1 mentions) Anti bruchpilot, by Developmental Studies Hybridoma Bank (1 mentions)

Spelling variants of this product

Mouse anti-GFP (4 citations) Anti-GFP (12A6) (2 citations)

2 protocols using anti gfp

1

Detailed Immunostaining Protocol using Candida Antibodies

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Primary antibodies: Mouse monoclonal: Anti-HA (BAbCO/Covance Research Products MMS-101R; HA.11 clone 16B12; IgG1); anti-β-1,3-glucan (Biosupplies Australia #400–2; IgG1); anti-Gfp (Developmental Studies Hybridoma Bank DSHB-GFP-4C9, -8H11 and -12A6); anti-β-1,2-mannotriose (B6.1 IgM, C3.1 IgG3 and G11.1 IgG1 [34 (link)–36 (link)]). Mouse antisera were all elicited by DNA vaccination with plasmids encoding Candida albicans polypeptides: Ywp1 aa 1–533, aa 21–116, aa 51–197 and aa 105–161; MP65 aa 17–378; Pho100 aa 18–323; and orf19.3621 aa 1–83 [22 (link)]. No monoclonal antibodies specific for Ywp1 are known to exist. Secondary antibodies: Goat anti-mouse IgG (H+L) conjugated to DTAF (dichlorotriazinyl aminofluorescein; Jackson ImmunoResearch), Alexa Fluor® 488 or Alexa Fluor® 568 (Molecular Probes); F(ab’)2 fraction of goat anti-mouse IgG (H+L) conjugated to Alexa Fluor® 594 (InVitrogen); monoclonal rat anti-mouse IgG1 conjugated to eFluor660 (eBioScience clone M1-14D12). Absorption/emission maxima (nm) of these fluorochromes: DTAF (493/519), Alexa Fluor® 488 (496/519), Alexa Fluor® 568 (578/603), Alexa Fluor® 594 (590/617), eFluor660 (633/668). The Alexa Fluor® 568 and 594 conjugates were not appropriate for the available flow cytometer lasers and filters, but otherwise usage was based largely on availability of the conjugates at the time of each experiment.
, & Granger B.L. (2018). Accessibility and contribution to glucan masking of natural and genetically tagged versions of yeast wall protein 1 of Candida albicans. PLoS ONE, 13(1), e0191194.
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2

Immunohistochemical Analysis of Olfactory Receptors

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The following primary mouse antibodies were used: anti-GFP (1:100), anti-Bruchpilot (1:50, nc82, supernatant, Developmental Studies Hybridoma Bank [DSHB] ), and anti Rab11 (1:100, BD Biosciences). The primary rabbit antibodies, anti-GFP (1:2,000, TP-401, Torrey Pines), Lamp1, anti-Rab5, anti-Rab11 (1:100, Abcam), anti-Or22a (1:10,000) and anti-Orco (1:10,000) were gifts from Richard Benton. The secondary antibodies were conjugated to Alexa 488 or Alexa 568 (1:500, Molecular Probes). Antenna immunohistochemistry was performed as described previously (Couto et al., ). Or22a images were captured from a subset of stereotypic Or22a sensilla opposite to the arista. The confocal microscopy images were collected on an LSM 700 (Zeiss) and analyzed on a Zen image browser.
Sanchez G.M., Alkhori L., Hatano E., Schultz S.W., Kuzhandaivel A., Jafari S., Granseth B, & Alenius M. (2016). Hedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila. Cell reports, 14(3).
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