Ab24192

Manufactured by Abcam

Sourced in United States, United Kingdom

Ab24192 is a lab equipment product. It is used for performing various scientific experiments and analyses in a laboratory setting. The core function of this product is to [core function description]. No further details or interpretation of intended use are provided.

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Lab products found in correlation

Blocking buffer, by Agilent Technologies (2 mentions) Bovine serum albumin (bsa), by Santa Cruz Biotechnology (2 mentions) Citrate based antigen unmasking solution, by Vector Laboratories (2 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (2 mentions) Alexa 488 green, by Thermo Fisher Scientific (2 mentions) Alexa 568 red, by Thermo Fisher Scientific (2 mentions) Eclipse te2000 u microscope, by Nikon (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Ab13876, by Abcam (1 mentions) Ab22048, by Abcam (1 mentions) Bond maxtm, by Leica camera (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions)

4 protocols using ab24192

Immunohistochemical Analysis of Toll-Like Receptors in Endometrial Pathologies

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Serial sections of diameter 3 microns were taken from the paraffin-embedded tissue samples of endometrial cancer, hyperplasia, and benign endometrium. One of these sections was stained with hematoxylin-eosin to confirm the diagnosis. Immunohistochemicals were applied to the tissue samples by an automated IHC/ISH stainer (Leica BOND-MAXTM, New Castle, UK). An anti-TLR2 antibody (anti-TLR2 antibody ab24192, ABCAM, Cambridge, MA, USA), an anti-TLR4 antibody (anti-TLR4 antibody [76B357.1] ab22048, ABCAM, Cambridge, MA, USA), an anti-TLR5 antibody (anti-TLR5 antibody [19D759.2] ab13876, ABCAM, Cambridge, MA, USA), and an anti-TLR6 antibody (anti-TLR6 antibody ab59920, ABCAM Cambridge, MA, USA) were used.
Immunohistochemical evaluation was performed in a semiquantitative manner by the same pathologist. The distribution and intensity of the TLR 2, 4, 5, and 6 staining of the cells were evaluated and compared with the background tumor cells. Staining of the nucleus and/or cytoplasm was considered a positive result. According to the distribution and intensity of the immunohistochemical staining, the samples were subjectively divided into four groups: group 0: no staining, group 1: mild staining, group 2: moderate staining, and group 3: severe staining. The SPSS 16.0 (Chicago, IL) package program was used for statistical analysis, and p<0.05 was considered significant.

Gençoğlu Bakbak B.B., Ilhan T.T., Pekin A., Kerimoğlu Ö.S., Yılmaz S.A., Kebapçılar A., Doğan N.U., Karabağlı P, & Çelik Ç. (2018). Evaluation of Toll-Like Receptor Expression with Clinicopathologic Variables in Endometrium Cancer. The Medical Bulletin of Sisli Etfal Hospital, 52(3), 196-200.

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Immunostaining for TLR2 and GFAP Co-Expression

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A standard technique was followed for immunohistochemistry to detect TLR2 and GFAP co-expression [17 (link)]. Formalin-fixed, paraffin-embedded tissues were sectioned at 6 μm and mounted onto positively charged glass slides. Sections were baked for overnight at 60°C, deparaffinized in xylene, and then rehydrated in graded concentrations of ethanol. Antigen retrieval was carried out for 20 min using a steamer and a citrate-based antigen unmasking solution (Vector Labs, Burlingame, CA). Tissues were blocked in blocking buffer (Dako) for one hour at room temperature before antibodies were applied. Tissues were incubated with TLR2 (ab24192, Abcam) and GFAP primary antibody (GA-5, Sigma) overnight at 4°C, washed three times with PBS with 0.2% bovine serum albumin (Santa Cruz) (PBS/BSA), and then incubated in the dark for 60 min at room temperature with secondary antibodies directly conjugated with Alexa 488 (green) or Alexa 568 (red) (Molecular Probes/Invitrogen, Carlsbad, CA). Sections were washed three times in PBS/BSA, cover-slipped with Prolong Gold with DAPI (Molecular Probes/Invitrogen), and imaged on a Nikon Eclipse TE2000-U microscope.

Inglis F.M., Lee K.M., Chiu K.B., Purcell O.M., Didier P.J., Russell-Lodrigues K., Weaver S.C., Roy C.J, & MacLean A.G. (2015). Neuropathogenesis of Chikungunya Infection: Astrogliosis and Innate Immune Activation. Journal of neurovirology, 22(2), 140-148.

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Immunofluorescence Staining of Formalin-Fixed Tissues

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Formalin-fixed, paraffin-embedded tissues were sectioned at 6μm and mounted onto positively charged glass slides. Sections were baked for overnight at 60°C, deparaffinized in xylene, and then rehydrated in graded concentrations of ethanol. Antigen retrieval was carried out for 20 min using a steamer and a citrate-based antigen unmasking solution (Vector Labs, Burlingame, CA). Tissues were blocked in blocking buffer (Dako) for one hour at room temperature before antibodies were applied. Tissues were incubated with TLR2 (ab24192, Abcam) and glial fibrillary acidic protein (GFAP) primary antibody (GA-5, Sigma) overnight at 4°C, washed three times with PBS with 0.2% bovine serum albumin (Santa Cruz) (PBS/BSA), and then incubated in the dark for 60 min at room temperature with secondary antibodies directly conjugated with Alexa 488 (green) or Alexa 568 (red) (Molecular Probes/Invitrogen, Carlsbad, CA). Sections were washed three times in PBS/BSA, cover-slipped with Prolong Gold with DAPI (Molecular Probes/Invitrogen), and imaged on a Nikon Eclipse TE2000-U microscope.

Lee K.M., Chiu K.B., Didier P.J., Baker K.C, & MacLean A.G. (2015). Naltrexone treatment reverses astrocyte atrophy and immune dysfunction in self-harming macaques. Brain, behavior, and immunity, 50, 288-297.

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Quantification of Cell Signaling Proteins

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Antibodies against vimentin (sc6260), α-SMA (sc53015), GAPDH (sc47724), E-cadherin (sc7870), RAGE (sc365154), and TLR4 (sc10741) (Santa Cruz Biotechnology, Dallas, TX, USA); against occludin (33-1500) and ZO-1 (40-2300) (Invitrogen [Thermo Fisher], Carlsbad, CA, USA); and against TLR2 (ab24192) (Abcam, Cambridge, UK) were all purchased, as well as enzyme-linked immunosorbent assay (ELISA) kits for human TGF-β (R&D Systems Inc, Minneapolis, MN, USA) and human HMGB1 (Shino-Test Corp, Kanagawa, Japan). RIPA lysis buffer and a BCA protein assay kit were supplied by Thermo Fisher Scientific (Waltham, MA, USA). Acn enhanced chemiluminescent (ECL) immunodetection system (Amersham, Little Chalfont, Buckinghamshire, UK) was also purchased, and recombinant wild-type HMGB1 protein was the product of CHO cells (A&R Therapeutics, Daejeon, Republic of Korea).

Min H.J., Choe J.W., Kim K.S., Yoon J.H, & Kim C.H. (2020). High-mobility group box 1 protein induces epithelialmesenchymal transition in upper airway epithelial cells. Rhinology, 58(5).

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