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Deltamethrin

Manufactured by Merck Group
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Sourced in United States, Germany, China

Deltamethrin is a laboratory-grade synthetic pyrethroid insecticide. It is used as a standard reference compound in analytical applications, particularly for the detection and quantification of pyrethroids in environmental and food samples.

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Lab products found in correlation

Permethrin, by Merck Group (11 mentions) Cypermethrin, by Merck Group (7 mentions) Imidacloprid, by Merck Group (6 mentions) Chlorpyrifos, by Merck Group (5 mentions) Acetone, by Merck Group (5 mentions) Malathion, by Merck Group (4 mentions) Bifenthrin, by Merck Group (4 mentions) Penconazole, by Merck Group (3 mentions) Esfenvalerate, by Merck Group (3 mentions) Cyhalothrin, by Merck Group (3 mentions) Fenpropathrin, by Merck Group (3 mentions) Fenvalerate, by Merck Group (3 mentions) Methanol, by Merck Group (3 mentions) Bendiocarb, by Merck Group (3 mentions) Lambda cyhalothrin, by Merck Group (3 mentions) Okadaic acid, by Merck Group (3 mentions) Alpha cypermethrin, by Merck Group (3 mentions) Chlorpyrifos methyl, by Merck Group (3 mentions) Fipronil, by Merck Group (3 mentions) Diazinon, by Merck Group (3 mentions)

Close spelling variants of this product

Technical grade deltamethrin (3 citations) Deltamethrin PESTANAL (2 citations) α-S)-deltamethrin (2 citations)

48 protocols using deltamethrin

1

Deltamethrin Exposure Effects on Crabs

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In October 2016, healthy mature male crabs (average weight, 114.04 ± 9.14 g) were collected from the crab breeding base (32.864256110°N, 119.866714447°E) of Tao Huadao Agricultural Development Co., Ltd. in Anfeng Town, Xinghua City, northern Jiangsu Province, China. The crabs were transported to the laboratory in polystyrene boxes filled with cultivation water, which was aerated during transportation with an aeration pump. Upon arrival, the crabs were cultured in experimental tanks (capacity, 90 L) with 20 L of natural water under controlled temperature (23–25°C), pH (7.25 ± 0.25), and dissolved oxygen (6.85 ± 0.15 mg·L-1) for 2 weeks. During the acclimation period, the crabs were fed with a commercial crab feed (37% crude protein) 2 times a day and were starved 1 day before the experiment. After 2 weeks of acclimation, 60 healthy crabs were separated into 6 groups (3 test and 3 control groups); the crabs in the test groups were given a 40 min bath in 3 ppb of deltamethrin (95% purity, Sigma; dissolved in acetone to prepare a stock solution of 25 mg·L-1), and the crabs in the control groups were exposed to the same concentration of acetone as that of deltamethrin in the test groups. Each experiment was performed in triplicate. After 40 min, the crabs were dissected on ice, and the hepatopancreas was collected in liquid nitrogen and stored at -80°C for RNA extraction.
Yang Z., Zhang Y., Jiang Y., Zhu F., Zeng L., Wang Y., Lei X., Yao Y., Hou Y., Xu L., Xiong C., Yang X, & Hu K. (2017). Transcriptional responses in the hepatopancreas of Eriocheir sinensis exposed to deltamethrin. PLoS ONE, 12(9), e0184581.
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2

Deltamethrin Exposure Protocol

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Deltamethrin (Sigma, St. Louis, MO) is the reference pyrethroid selected by the EPA (EPA, 2011). Aliquots were prepared by dissolving Deltamethrin (Sigma, St. Louis, MO) in acetone (Sigma), mixing with corn oil (Mazola, Memphis, TN), allowing the acetone to evaporate overnight, and then storing at −80° C in capped borosilicate glass vials (Fisher Scientific, Hampton, NH) until the day of use. Aliquots of vehicle were prepared by allowing a similar volume of acetone to evaporate in corn oil.
Curtis M.A., Saferin N., Nguyen J.H., Imami A.S., Ryan W.G., Neifer K.L., Miller G.W, & Burkett J.P. (2024). Developmental pyrethroid exposure disrupts folate metabolism in mouse brain. bioRxiv.
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3

Deltamethrin Dose Preparation for Rat Studies

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Aliquots of deltamethrin (Sigma, St. Louis, MO) in corn oil (Mazola, Memphis, TN) were prepared at a concentration of 9 mg/mL by dissolving deltamethrin powder in acetone (Sigma), mixing the acetone with corn oil, evaporating the acetone from the mixture overnight in a fume hood, and storing the resulting corn oil in glass vials at −80° C. Vehicle aliquots were prepared using the same volumes of acetone and corn oil. Aliquots were thawed immediately prior to use. Dams were weighed immediately before each feeding and the appropriate amount of corn oil (1 μL/3 g body weight) was mixed with ~100 mg JIF peanut butter (J.M. Smucker, Orville, OH) to achieve a dose of 3 mg/kg.
Nguyen J.H., Curtis M.A., Imami A.S., Ryan W.G., Alganem K., Neifer K.L., Saferin N., Nawor C.N., Kistler B.P., Miller G.W., Shukla R., McCullumsmith R.E, & Burkett J.P. (2024). Developmental pyrethroid exposure disrupts molecular pathways for MAP kinase and circadian rhythms in mouse brain. bioRxiv.
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4

Characterization of Na+v1.7 Expressing HEK-293 Cells

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FMP blue dye was obtained from Molecular Devices (Sunnyvale, CA, USA). ATX was synthesized as described previously and was characterized to be above 95% purity [59 (link)]. G-418, penicillin, streptomycin, heat inactivated fetal bovine serum, poly-d-lysine (molecular weight >300,000), riluzole, veratridine and carbamazepine, deltamethrin, PbTx-2, TTX, and lamotrigine were obtained from Sigma-Aldrich (St. louis, MO, USA). SKA-19 was provided by Prof. Wulff at the University of California, Davis as described previously [28 (link)] and was characterized to be greater than 95% purity. Lidocaine was purchased from Abcam (Cambridge, MA, USA). The HEK-293 stably expressed Nav1.7 was a generous gift from Dr. Lossin (University of California, Davis) and was the same line used as described previously [28 (link)].
Zhao F., Li X., Jin L., Zhang F., Inoue M., Yu B, & Cao Z. (2016). Development of a Rapid Throughput Assay for Identification of hNav1.7 Antagonist Using Unique Efficacious Sodium Channel Agonist, Antillatoxin. Marine Drugs, 14(2), 36.
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5

Pesticide Effects on Mitochondrial Function

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Mitochondrial pesticides chlorfenapyr (CAS Number 122453-73-0), hydramethylnon (CAS Number 67485-29-4), pyridaben (CAS Number 96489-71-3), tolfenpyrad (CAS Number 129558-76-5), and fenazaquin (CAS Number 120928-09-8) and non-mitochondrial pesticides imidacloprid (CAS Number 138261-41-3), deltamethrin (CAS Number 52918-63-5), malathion (CAS Number 121-75-5), metribuzin (CAS Number 21087-64-9), penconazole (CAS Number 66246-88-6), cypermethrin (CAS Number 52315-07-8), and esfenvalerate (CAS Number 66230-04-4) were from Sigma-Aldrich, USA. All pesticides were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mM.
Syromyatnikov M., Nesterova E., Smirnova T, & Popov V. (2021). Methylene blue can act as an antidote to pesticide poisoning of bumble bee mitochondria. Scientific Reports, 11, 14710.
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6

QuEChERS Extraction and UHPLC-PDA Analysis of Pesticides

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All chemicals and solvents were of analytical quality grade. HPLC grade acetonitrile (MeCN) and methanol (MeOH) were purchased from LabScan (Dublin, Ireland). Insecticide standards, chlorpyrifos (98.3%), acrinathrin (99.7%), deltamethrin (99.9%) and ʎ-cyhalothrin (≥95.0%) were supplied by Sigma-Aldrich (St. Louis, MO, USA), Table 1. Buffered salts for QuEChERS extraction, including anhydrous magnesium sulphate (MgSO4), sodium hydroxide (NaOH), sodium chloride (NaCl), trisodium citrate dihydrate (C6H5Na3O7·2H2O) and disodium hydrogencitrate sesquihydrate (C6H8Na2O8), were also obtained from Sigma-Aldrich. dSPE clean-up tubes with primary secondary amine (PSA) and MgSO4 were obtained from Waters (Milford, MA, USA). Phosphoric acid (PhA, ≥85%) was from BDH (Poole, UK), while formic acid (FA, ≥99%) was obtained from Merck (Darmstadt, Germany). Ultrapure water (H2O) from a Milli-Q ultrapure water purification system (Millipore, Bedford, USA) was used for preparing the UHPLC mobile phase and other aqueous solutions. Filters of 13 mm with 0.22-µm PTFE membranes were used for filtration of the final extracts before UHPLC-PDA analysis.
Reis D., Silva P., Perestrelo R, & Câmara J.S. (2020). Residue Analysis of Insecticides in Potatoes by QuEChERS-dSPE/UHPLC-PDA. Foods, 9(8), 1000.
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7

Deltamethrin Exposure in Channel Catfish

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Deltamethrin (purity > 98%) was obtained from Sigma Aldrich (Chemical Co., USA), then dissolved in dimethyl sulfoxide (DMSO) as a stock solution. All healthy juvenile channel catfish (100 ± 20 g) were purchased in the Wuhan Baishazhou aquatic market. Before the experiment, these fish were domesticated for one week under laboratory conditions free of specific pathogens at 25 ± 1°C.
Yang Y., Zhu X., Huang Y., Zhang H., Liu Y., Xu N., Fu G, & Ai X. (2022). RNA-Seq and 16S rRNA Analysis Revealed the Effect of Deltamethrin on Channel Catfish in the Early Stage of Acute Exposure. Frontiers in Immunology, 13, 916100.
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8

Characterizing Ion Channel Modulators

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BTX and TTX were from Latoxan (Valence, France). VTD was from Santa Cruz Biotechnology (Dallas, TX, USA), Tf2 and pompilitoxin (β-PMTX) were purchased from Smartox Biotechnology (Saint Egrève, France) and PbTx-2 was a gift from Andrea Bourdelais (University of North Carolina Wilmington, Wilmington, NC, USA). BIII 890 CL, such as BI 55 CL, was kindly provided by Boehringer Ingelheim (Biberach an der Riss, Germany) and Bay K8644 was from Alomone (Jerusalem, Israel). All other reagents and solvents, including Fura-2 AM, Pluronic®-F127 acid, ICA-121431, SN-6, SEA-0400, deltamethrin, thapsigargin, nifedipine, and cadmium chloride (CdCl2), were obtained from Sigma-Aldrich Merck (Saint-Louis, MO, USA) or Thermo Fisher Scientific (Waltham, MA, USA).
Réthoré L., Park J., Montnach J., Nicolas S., Khoury J., Le Seac’h E., Mabrouk K., De Pomyers H., Tricoire-Leignel H., Mattei C., Henrion D., Fajloun Z., De Waard M., Legendre C, & Legros C. (2022). Pharmacological Dissection of the Crosstalk between NaV and CaV Channels in GH3b6 Cells. International Journal of Molecular Sciences, 23(2), 827.
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9

Pesticide Biodegradation Assay Protocol

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Technical-grade permethrin, deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin were obtained from Sigma-Aldrich, USA. Chromatographic grade acetonitrile was purchased from Fisher Scientific, USA. All other reagents used here were obtained from Sigma-Aldrich, USA. The chemicals were dissolved in acetone or dimethyl sulfoxide (DMSO) at a stock concentration of 10 g·L−1, and stored in dark bottles at 4°C prior to use.
The mineral salt medium (MSM) containing 2 g (NH4)2SO4, 0.2 g MgSO4·7H2O, 0.01 g CaCl2·2H2O, 0.001 g FeSO4·7H2O, 1.5 g Na2HPO4·12H2O, and 1.5 g KH2PO4 per liter was used for biodegradation assays in the study. The Luria-Bertani medium (LB) containing 10 g tryptone, 5 g yeast extract and 10 g NaCl per liter was used for incubating Acinetobacter baumannii ZH-14. Both of the media were autoclaved at 121°C for 20 min. The pH of the media was adjusted to 7.0.
Zhan H., Wang H., Liao L., Feng Y., Fan X., Zhang L, & Chen S. (2018). Kinetics and Novel Degradation Pathway of Permethrin in Acinetobacter baumannii ZH-14. Frontiers in Microbiology, 9, 98.
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10

Deltamethrin Exposure and Breeding in Mice

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Eight-week-old C57BL/6NCrl wild-type mice (Charles River Labs) received food and water ad libitum and were maintained on a 12:12 dark/light cycle. Females were dosed with 3 mg/kg deltamethrin (Sigma-Aldrich) every three days for two weeks prior to breeding in triplicate. deltamethrin dosing continued during breeding and gestation. One male and one female mouse per litter were utilized for each experiment to reduce confounding due to litter effects. Throughout, an independent litter represents one sample (n = 1). All experiments were approved by the Institutional Animal Care and Use Committee at Emory University and were conducted in accordance with the National Institutes of Health Guide of Care and Use of Laboratory Animals.
Vester A.I., Hermetz K., Burt A., Everson T., Marsit C.J, & Caudle W.M. (2020). Combined neurodevelopmental exposure to deltamethrin and corticosterone is associated with Nr3c1 hypermethylation in the midbrain of male mice. Neurotoxicology and teratology, 80, 106887.
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