Lysotracker red

Manufactured by Yeasen

Sourced in China

LysoTracker® Red is a fluorescent dye that selectively stains acidic organelles, such as lysosomes, in live cells. It is a useful tool for studying lysosome function and distribution within the cell.

Automatically generated - may contain errors

Lab products found in correlation

Chlorpromazine, by Merck Group (3 mentions) Amiloride, by Merck Group (2 mentions) Genistein, by Merck Group (2 mentions) Mitotracker red, by Yeasen (2 mentions) Kgmp0026, by Keygen Biotech (2 mentions) Hepes, by Merck Group (1 mentions) Dspe peg2000 mal, by Xi’an Ruixi (1 mentions) Goat anti rabbit igg h l alexa fluor 488 antibody, by Abcam (1 mentions) Anti acetyl histone h3, by Merck Group (1 mentions) Alkaline phosphatase alp, by Yeasen (1 mentions) β alanine, by Solarbio (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Fmoc amino acids, by GL Biochem (1 mentions) Anti acetyl histone h4 lys8, by Merck Group (1 mentions) Parp rabbit mab, by Cell Signaling Technology (1 mentions) 2 chlorotrityl chloride resin, by GL Biochem (1 mentions) β actin mouse mab, by Proteintech (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Coumarin 6, by Merck Group (1 mentions)

Spelling variants of this product

LysoTracker Red DND-99 (11 citations) Lysotracker (5 citations)

6 protocols using lysotracker red

Lipid-based Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

1.2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1.2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), and cholesterol and cholesteryl hemisuccinate (CHEMS) were purchased from A.V.T. (Shanghai, China). 1.2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG₂₀₀₀) was purchased from NOF Corporation (Tokyo, Japan). DSPE-PEG₂₀₀₀-MAL was purchased from Xi’an ruixi Biological Technology (Xi’an, China). HEPES, EDTA·2Na·2H₂O and doxycycline hyclate (DOX·HCl) were supplied by Sigma-Aldrich (Shanghai, China). DVar7 ^D(ACEEQNOWARYLEWLFPTETLLLEL) was synthesized by GL Biochem (Shanghai, China). LysoTracker Red was purchased from Shanghai Yeasen Biotech (Shanghai, China). The other chemicals and reagents were analytical grade.

Zhai L., Luo C., Gao H., Du S., Shi J, & Wang F. (2021). A Dual pH-Responsive DOX-Encapsulated Liposome Combined with Glucose Administration Enhanced Therapeutic Efficacy of Chemotherapy for Cancer. International Journal of Nanomedicine, 16, 3185-3199.

+ Open protocol

+ Expand

Protein Expression and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fmoc-amino acids and 2-chlorotrityl chloride resin were purchased from GL Biochem (Shanghai, China). β-Alanine, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl), crystal violet, bovine serum albumin (BSA), DAPI, propidium iodide (PI), l-phenylalanine and other chemical reagents were bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Alkaline phosphatase (ALP) and Lyso-Tracker Red were obtained from Yeasen Biotechnology (Shanghai, China). Dulbecco's modified Eagle's medium (DMEM), RPMI Medium 1640 basic and fetal bovine serum (FBS) were obtained from Gibco (Suzhou, China). Anti-γH2AX (phosphor A139) antibody and goat anti-rabbit IgG H&L (Alexa Fluor® 488) antibody were obtained from Abcam (Shanghai, China). PARP rabbit mAb, cleaved PARP (Asp214) rabbit mAb and caspase 3 rabbit antibody were purchased from Cell Signaling Technology (Shanghai, China). Anti-acetyl histone H3 and anti-acetyl histone H4 (Lys 8) antibodies were provided by Millipore (Billerica, USA). β-Actin Mouse mAb and peroxidase-conjugated affinipure goat anti-mouse/rabbit IgG (H + L) were bought from Proteintech Group Inc. (Wuhan, China). FITC Annexin V apoptosis detection kit was purchased from BD Pharmingen (San Diego, CA, USA). Dynasore was purchased from TCI Development Co., Ltd. (Shanghai, China).

Gao Y., Gao J., Mu G., Zhang Y., Huang F., Zhang W., Ren C., Yang C, & Liu J. (2020). Selectively enhancing radiosensitivity of cancer cells via in situ enzyme-instructed peptide self-assembly. Acta Pharmaceutica Sinica. B, 10(12), 2374-2383.

+ Open protocol

+ Expand

Synthesis and Characterization of Pluronic P85-TPP Conjugate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pluronic P85 (M.W. = 4600) was obtained from BASF Aktiengesellschaft, and (5-carboxypentyl)triphenylphosphonium bromide (TPP-COOH) was provided by Bailingwei Co., Ltd. (Guangzhou, China). GSH (reduced) and DA were provided by Energy Chemical Co., Ltd. (Shanghai, China). Bis(2-hydroxyethyl) disulfide was obtained from TCI Development Co., Ltd. (Shanghai, China). N,N′-Dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), genistein, chlorpromazine, amiloride, coumarin 6 (C6), and PTX were supplied by Sigma-Aldrich (Shanghai, China). Penicillin-streptomycin, fetal bovine serum (FBS), and RPMI 1640 were purchased from Gibco Life Technologies (USA). An ATP assay kit was provided by Beyotime Biotechnology. MitoTracker Red and LysoTracker® Red were supplied by Yeasen Biotech Co., Ltd. (Shanghai, China). All other reagents and solvents were of analytical grade and were used without further purification.

Wang H., Shi W., Zeng D., Huang Q., Xie J., Wen H., Li J., Yu X., Qin L, & Zhou Y. (2021). pH-activated, mitochondria-targeted, and redox-responsive delivery of paclitaxel nanomicelles to overcome drug resistance and suppress metastasis in lung cancer. Journal of Nanobiotechnology, 19, 152.

+ Open protocol

+ Expand

Synthesis and Characterization of 4-Arm PEG

Check if the same lab product or an alternative is used in the 5 most similar protocols

4-Arm PEG (M_w = 5000 Da) was purchased from Macklin Co., Ltd. (Guangzhou, China). COA (batch number, 130506XF; purity >99%) was purchased from Shanghai KANGLANG Biomedical Ltd (Shanghai, China). 4-Dimethylaminopyridine (DMAP), N,N′-dicyclohexylcarbodiimide (DCC), coumarine-6 (C6), amiloride, genistein, chlorpromazine, GSH (reduced), and PTX were purchased from Sigma–Aldrich (Shanghai, China). Bis(2-hydroxyethyl) disulfide was obtained from TCI Development Co., Ltd. (Shanghai, China). FBS and RPMI 1640 medium were purchased from HyClone and Gibco, respectively (Thermo Fisher Scientific Co., Ltd., Shanghai, China). The near-infrared (NIR) fluorescent dye DIR was supplied by Keygen Biotech (KGMP0026, China). Hematoxylin and eosin (H&E) staining reagents were supplied by Leagene Biology Technology Co., Ltd. (Beijing, China). LysoTracker® Red was purchased from Yeasen Biotech Co., Ltd (Shanghai, China). All other reagents were of analytical grade.

Zhou Y., Miao Y., Huang Q., Shi W., Xie J., Lin J., Huang P., Yue C., Qin Y., Yu X., Wang H., Qin L, & Chen J. (2022). A redox-responsive self-assembling COA-4-arm PEG prodrug nanosystem for dual drug delivery suppresses cancer metastasis and drug resistance by downregulating hsp90 expression. Acta Pharmaceutica Sinica. B, 13(7), 3153-3167.

+ Open protocol

+ Expand

Cholesterol and Chloroquine Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cholesterol and chloroquine
(CQ) were supplied
by Sigma-Aldrich Co. Ltd. Octaethylene glycol monododecyl ether and
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC) were supplied by TCI. Zirconyl chloride octahydrate (ZrOCl₂·8H₂O) and benzoic acid (BA) were purchased
from Sinopharm Chemical Reagent Co., Ltd. Tetrakis (4-carboxyphenyl)
porphyrin (TCPP) was synthesized according to the previous report.^{41 (link)} The synthesis of squaramide (3,4-bis(4-(trifluoromethyl)phenylamino)cyclobut-3-ene-1,2-dione,
SQU) was based on the steps in the previous literature.^{42 (link)} LysoTracker Red, LysoSensor Green DND-189, and
annexin V-FITC/PI cell apoptosis kit were supplied by Yeasen, Shanghai,
China. The 2′,7′-dichlorofluorescin diacetate (DCFH-DA),
LysoTracker Red, N-(ethoxycarbonylmethyl)-6-methoxyquinolinium
bromide (MQAE), and ZVAD-FMK were obtained from Beyotime Institute
of Biotechnology. JC-1 and Calcein-AM were purchased from 4A Biotech
Co., Ltd.

Wan S.S., Zhang L, & Zhang X.Z. (2019). An ATP-Regulated Ion Transport Nanosystem for Homeostatic Perturbation Therapy and Sensitizing Photodynamic Therapy by Autophagy Inhibition of Tumors. ACS Central Science, 5(2), 327-340.

+ Open protocol

+ Expand

Pluronic-Based Nanoparticle Drug Delivery

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pluronic F127 (Mw = 12,600) was purchased from BASF aktiengesellschaft, and (5-carboxypentyl)triphenylphosphonium bromide (TPP-COOH) was purchased from Bailingwei Co., Ltd., Guangzhou, China. HA and HAase were acquired from J&K Scientific Ltd. (Beijing, China). N,N′-dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), genistein, chlorpromazine, amiloride, coumarine-6 (C6), and PTX were purchased from Sigma-Aldrich (Shanghai, China). Fetal bovine serum (FBS) and RPMI 1640 medium were purchased from HyClone and Gibco, respectively (Thermo Fisher Scientific Co., Ltd., Shanghai, China). The near-infrared (NIR) fluorescent dye DIR was supplied by Keygen Biotech (KGMP0026, China). Hematoxylin and eosin (H&E) staining reagents were supplied by Leagene Biology Technology Co., Ltd. (Beijing, China). MitoTracker Red and LysoTracker^® Red were purchased from Yeasen Biotech Co., Ltd. (Shanghai, China). All other reagents were of analytical grade.

Wang H., Zhang F., Wen H., Shi W., Huang Q., Huang Y., Xie J., Li P., Chen J., Qin L, & Zhou Y. (2020). Tumor- and mitochondria-targeted nanoparticles eradicate drug resistant lung cancer through mitochondrial pathway of apoptosis. Journal of Nanobiotechnology, 18, 8.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!