E coli inorganic pyrophosphatase

Oligonucleotides were obtained from TsingKe (Beijing, China). DNase I, restriction endonucleases, E. coli inorganic pyrophosphatase, E. coli Poly(A) Polymerase, RNA 5′ Pyrophosphohydrolase (RppH), T4 DNA ligase, NTPs, dNTPs, and RNA purification kits were from New England BioLabs (Ipswich, MA, United States). RNase inhibitor was from Thermo Fisher Scientific (Waltham, MA, United States). PrimeSTAR Max DNA Polymerase and Premix Taq DNA Polymerase, SMARTScribe and ProtoScript II Reverse Transcriptase are were from TAKARA (Shiga, Japan). DNA purification kit was from Axygen (Union City, CA, United States). Ni-NTA resin was from Qiagen (Hilden, Germany). Preparative Superdex S200 for gel filtration was from GE Healthcare (Chicago, IL, United States). Radiolabeled nucleotides were from PerkinElmer (Waltham, MA, United States). 2′-F-dNTPs were from TriLink (San Diego, CA, United States).

Oligonucleotides were obtained from Integrated DNA Technology (Oligonucleotides less than 60 nt were ordered at the 25 nmole scale and those longer than 60 nt were ordered at the 4 nmole Ultramer scale). DNA purification kits and Ni-NTA resin were from Qiagen. Preparative Superdex 200 for gel filtration was from GE Healthcare. Restriction endonucleases, T4 DNA ligase, Vent_R^® DNA Polymerase, Q5^® Site-Directed Mutagenesis Kit, T7 RNA polymerase and E. coli inorganic pyrophosphatase were from New England Biolabs. T7 R&DNA™ Polymerase (T7-Y639F RNA polymerase) was from Epicentre. DNA Clean & Concentrator™-5 kit was from ZYMO Research. RNaseOUT™ recombinant ribonuclease inhibitor was from Invitrogen. NTPs were from USB and 2′-F-dNTPs were from Trilink.