Agilent 244k

HT1080 human fibrosarcoma cells and SK-LMS-1 human leiomyosarcoma cells were obtained from the American Type Culture Collection (Manassas, VA). The DDLS8817 dedifferentiated liposarcoma cell line was established from samples obtained from patients who signed informed consent forms and were confirmed by cytogenetic analysis and by DNA copy number arrays (Agilent 244 K) to harbor 12q amplification. HT1080 and SK-LMS-1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), and DDLS8817 cells were maintained in DMEM-F12. All media were supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL), streptomycin (100 μg/mL), and L-glutamine (2 mM) (“regular media”). Cancer cell lines were actively passaged for <6 months from the time that they were received, and United Kingdom Coordinating Committee on Cancer Research (UKCCCR) guidelines were followed [37 ]. Doxorubicin (RYG02) was purchased from TSZ Chem/BIOTANG Inc. (Lexington, MA). LY294002 (Cat. 440202), U0126 (Cat. 662005), and puromycin (Cat. 508838) were purchased from Calbiochem.

HT1080 human fibrosarcoma cells and SK-LMS-1 human leiomyosarcoma cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA). The DDLS8817 dedifferentiated liposarcoma cell line was established from a patient sample following informed consent and were confirmed by cytogenetic analysis and by DNA copy number arrays (Agilent 244K) to harbor chromosome 12q amplification. HT1080 and SK-LMS-1 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM), and DDLS8817 cells were maintained in DMEM-F12. All media were supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL), streptomycin (100 μg/mL), and l-glutamine (2 mM) (hereafter termed “regular media”). Cancer cell lines were actively passaged for less than 6 months from the time that they were received, following United Kingdom Coordinating Committee on Cancer Research guidelines^{56 (link)}. Doxorubicin (RYG02) was purchased from Biotang Inc. (Lexington, MA). LY294002 (cat. 440202) and Puromycin (cat. 508838) were purchased from Calbiochem.

Total genomic DNA was isolated from fresh frozen lymphoma samples or cytogenetic pellet (Table 1; case 2) using standard procedures. Genomic profiling, following the manufacturer's protocols, was performed using the Agilent 244k (www.agilent.com) (5 cases) and the Affymetrix CytoScan HD arrays (www.affymetrix.com) (4 cases). Array CGH data are available at GEO (Accession number: GSE57944).