Mr ab64457

Manufactured by Cell Signaling Technology

The MR (ab64457) is a lab equipment product from Cell Signaling Technology. It serves as a core function for biological research applications.

Automatically generated - may contain errors

Lab products found in correlation

Tubulin, by Merck Group (1 mentions) Secondary peroxidase conjugated anti rabbit na934 and anti mouse na931 antibodies, by GE Healthcare (1 mentions) Actin, by Merck Group (1 mentions) P38α sc 535, by Santa Cruz Biotechnology (1 mentions) E cadherin, by BD (1 mentions) Anti goat, by Jackson ImmunoResearch (1 mentions)

2 protocols using mr ab64457

Antibody Validation for Transcription Factors

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Antibodies were from Biolegend: K5 (PRB-160P), Loricrin (PRB-145P); Santa Cruz Biotechnology: p65-NF-κB (sc-372), β-catenin (sc-7199), MR (sc-11412 for ChIP), GR (sc-1004), HSD11B2 (sc-20176); Abcam: MR (ab64457; for immunohistochemistry); Cell Signalling: p-GR (Ser211; #4161), LaminA/C (#2032); and Sigma: Actin (A-2066), and Tubulin (T6199). Secondary biotin-conjugated anti-rabbit antibody (Jackson ImmunoResearch, West Grove, PA) and secondary Alexa Fluor® anti-rabbit (555, A-31572) antibody (Thermo Fisher) were used for immunostaining. Secondary peroxidase-conjugated anti-rabbit (NA934) and anti-mouse (NA931) antibodies (GE Healthcare) were used for immunoblotting.

Boix J., Bigas J., Sevilla L.M., Iacobone M., Citton M., Torresan F., Caroccia B., Rossi G.P, & Pérez P. (2017). Primary aldosteronism patients show skin alterations and abnormal activation of glucocorticoid receptor in keratinocytes. Scientific Reports, 7, 15806.

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Comprehensive Immunoblotting and Immunostaining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless otherwise specified all chemicals were from Sigma. Antibodies were from Biolegend: HA (MMS-101P), K5 (PRB-160P), K6 (PRB-169P), Loricrin (PRB-145P); Santa Cruz Biotechnology: p38α (sc-535), p63 (sc-8431), p65-NF-κB (sc-372), ERK (sc-154), GR (sc-1004), HSD11B2 (sc-20176), IκB(sc-371), Mkp1/2 (sc-1102), MR (sc-11412, ChIP), LaminA (sc-6214); Abcam: MR (ab64457); Cell Signalling Technology: p-ERK (Thr202/Tyr204; #4376), p-p38 (#9211S and #4631 for immunohistochemistry), p-JNK (#9251), JNK (#9252); ThermoFisher: GFP (A6455); Sigma: Tubulin (T6199), Actin (A2066); Roche: BrdU (11170376001) and BD Biosciences: E-cadherin (610181), Ly6G (551459). Secondary biotin-conjugated anti-rabbit, anti-mouse and anti-rat antibodies (Jackson ImmunoResearch) and secondary Alexa Fluor® anti-rabbit (555, A-31572) antibody (ThermoFisher) were used for immunostaining. Secondary peroxidase-conjugated anti-rabbit (NA934), anti-mouse (NA931) antibodies (GE Healthcare), and anti-goat (Jackson Immunoresearch) were used for immunoblotting.

Bigas J., Sevilla L.M., Carceller E., Boix J, & Pérez P. (2018). Epidermal glucocorticoid and mineralocorticoid receptors act cooperatively to regulate epidermal development and counteract skin inflammation. Cell Death & Disease, 9(6), 588.

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