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Avance 3 500 mhz nmr spectrometer

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The Avance III 500 MHz NMR spectrometer is a nuclear magnetic resonance (NMR) instrument manufactured by Bruker. It is designed to acquire and analyze high-resolution NMR spectra of various samples. The spectrometer operates at a frequency of 500 MHz and is capable of performing routine NMR experiments for chemical analysis and structural elucidation.

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Lab products found in correlation

Nicolet 5700 ftir instrument, by Thermo Fisher Scientific (3 mentions) Nicolet is50, by Thermo Fisher Scientific (3 mentions) 6210 lc tof spectrometer, by Agilent Technologies (2 mentions) Autoflex 3 maldi, by Bruker (2 mentions) Prostar 500 system, by Agilent Technologies (2 mentions) 335 uv vis detector, by Agilent Technologies (2 mentions) Sephadex lh 20, by GE Healthcare (2 mentions) Avance 3 700 mhz nmr spectrometer, by Bruker (2 mentions) 5 mm tci probe, by Bruker (2 mentions) Avance 3 600 mhz nmr spectrometer, by Bruker (2 mentions) Sigma 300, by Zeiss (2 mentions) Nicolet 380 ft ir, by Thermo Fisher Scientific (1 mentions) Compact qqtof, by Bruker (1 mentions) Cosmosil pack, by Nacalai Tesque (1 mentions) α glycosidase, by Merck Group (1 mentions) Amazon sl, by Bruker (1 mentions) Sephadex lh 20, by Merck Group (1 mentions) Avance 3 300 mhz nmr spectrometer, by Bruker (1 mentions) Rf 5301 spectrofluorophotometer, by Shimadzu (1 mentions) 363 fluorescence detector, by Agilent Technologies (1 mentions)

47 protocols using avance 3 500 mhz nmr spectrometer

1

Solid-state NMR Analysis of Silicon Compounds

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The 29Si magic angle spinning (MAS) spectra were obtained at 99 MHz by solid-state NMR using a Bruker Avance III 500 MHz NMR spectrometer, equipped with an HFX 4 mm Bruker MAS probe. Samples typically ∼150 mg were packed into a 4 mm zirconia rotor equipped with Kel-F caps. All samples were spun at 5 kHz. The 29Si MAS spectra were obtained by direct observation of 29Si with 1H decoupling and with a relaxation delay of 30 s. 29Si chemical shifts were referenced externally, with Tetrakis(trimethylsilyl)silane used as a standard (Si(CH3)3 at −9.3 ppm). The deconvolution software used was the OriginPro 9.0.0 SR2 b87 (Northampton, MA, USA).
Silva A., Nogueira R., Bogas A., Abrantes J., Wawrzyńczak D., Ściubidło A, & Majchrzak-Kucęba I. (2022). Valorisation of Recycled Cement Paste: Feasibility of a Short-Duration Carbonation Process. Materials, 15(17), 6001.
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2

Characterization of Organic Compounds

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Optical rotations were measured on a MCP 5100 modular compact polarimeter (Anton Paar, America). IR spectra were taken on a Nicolet 380 FT-IR instrument (Thermo, USA) as KBr discs. NMR spectra were recorded on a Bruker Avance III 500 MHz NMR spectrometer (Bruker, German). ESIMS and HRESIMS were recorded with amaZon SL (Bruker) or Compact QqTOF (Bruker). ECD spectra were measured by APL Chiascan (Applied Photophysics Ltd., England). Semi-preparative HPLC was carried out using a C18 column (Cosmosil-pack, 10 × 250 mm, 5 μm, 4 mL/min, Nacalai Tesque). C18 gel (20–45 μm, Fuji SilysiSa Chemical Co., Ltd., Greenville, NC, UA), Silica gel (60–80, 200–300 mesh, Qingdao Marine Chemical Co., Ltd., Qingdao, China), Sephadex LH-20 (Merck, Kenilworth, NJ, USA) were used for column chromatograghy. α-glycosidase (Sigma-Aldrich, UA) used for the bioactivity was derived from yeast and its EINECS (EC) unmber is 3.2.1.20.
Wang P., Chen X., Wang H., Huang S., Cai C., Yuan J., Zhu G., Xu X., Mei W, & Dai H. (2019). Four New Picrotoxane-Type Sesquiterpenes From Dendrobium nobile Lindl. Frontiers in Chemistry, 7, 812.
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3

Liver Metabolite Extraction and NMR Analysis

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Metabolites were extracted from livers in YNG (young group), OLD (old group), and HI (iron-overload group) following previously reported protocols (96 (link)). Briefly, approximately 200 mg of liver samples were homogenized in 2 ml ice-cold acetonitrile/water (1:1 v/v) buffer. After centrifugation (12,000g, 4 °C) for 10 min, the supernatant was collected into glass vials. Then most acetonitrile in the supernatant was removed using a gentle stream of nitrogen. Next, the aqueous residues were freeze-dried and stored at −80 °C until further analysis. For NMR analysis, the lyophilized samples were reconstituted in 600 μl heavy water phosphate buffer (0.2 M, pH 7.4) containing 0.05% trimethylsilylpropanoic acid as a chemical shift reference. NMR spectra were recorded on a Bruker AVANCE III 500 MHz NMR spectrometer at 298 K.
Liu J., Zhao Y., Ding Z., Zhao Y., Chen T., Ge W, & Zhang J. (2022). Iron accumulation with age alters metabolic pattern and circadian clock gene expression through the reduction of AMP-modulated histone methylation. The Journal of Biological Chemistry, 298(6), 101968.
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4

NMR-based Metabolic Profiling of Lyophilized Extracts

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According to published methods [10 (link)], the lyophilized extracts were dissolved in 600 μL of 99.8% D2O PBS buffer (pH 7.4) equipped with 0.05% (w/v) sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP). After vortexing, the mixtures were centrifuged at 12000 rpm for 15 min to discard sediments. The supernatants were transferred to new NMR tubes for 1H-NMR analysis.
The 1H-NMR spectra of samples were recorded on a Bruker AVANCE III 500 MHz NMR spectrometer at 298 K. D2O was used for field frequency locking, TSP was used as the chemical shift reference (1H, 0.00 ppm). A transverse relaxation-edited Carr-Purcell-Meiboom-Gill (CPMG) sequence [90(τ-180-τ) nacquisition] with a total spin-echo delay (2 nτ) of 40 ms was used to suppress the signals of proteins. 1H-NMR spectra were measured with 128 scans in 32 K data points with a spectral width of 10, 000 Hz. The spectra were Fourier transformed after multiplying the free induction decays (FIDs) by an exponential weighting function corresponding to a line-broadening of 0.5 Hz.
Luo H.Z., Guan Y., Yang R., Qian G.L., Yang X.H., Wang J.S, & Jia A.Q. (2020). Growth inhibition and metabolomic analysis of Xanthomonas oryzae pv. oryzae treated with resveratrol. BMC Microbiology, 20, 117.
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5

Synthesis and Characterization of Supramolecular Hosts

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All reagents were commercially available and used without further purification. TPE-(Br)4, G1, M1 and MCP5 were synthesized according to a published literature procedure (See the Supporting Information for details)20 (link),37 (link),50 (link),62 (link). 1H NMR spectra were collected on a Bruker AVANCE III 300 MHz NMR spectrometer. 13C NMR, 2D NOESY NMR and DOSY NMR spectra were recorded on a Bruker AVANCE III 500 MHz NMR spectrometer. High-resolution electrospray ionization mass spectra (HRESI-MS) were obtained on a Bruker 7-Tesla FT-ICR mass spectrometer equipped with an electrospray source. Mass spectra were recorded on Bruker Daltonics Autoflex Speed Series: High-Performance MALDI-TOF Systems. FT-IR spectra were recorded on a Vertex 80 V spectrometer. Scanning electron microscope (SEM) images were obtained on a HITACHI-SU8082 instrument. The fluorescence experiments were conducted on a RF-5301 spectrofluorophotometer (Shimadzu Corporation, Japan). To determine the stoichiometry and association constants of alcohols⊂MSP5, 1H NMR titration was performed. By a nonlinear curve-fitting method, the association constants between the guests and host were calculated. Through a molar ratio plot, the stoichiometry was determined (see supporting information for details).
Jin X.Y., Song N., Wang X., Wang C.Y., Wang Y, & Yang Y.W. (2018). Monosulfonicpillar[5]arene: Synthesis, Characterization, and Complexation with Tetraphenylethene for Aggregation-Induced Emission. Scientific Reports, 8, 4035.
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6

Synthesis and Characterization of Gd(III) Contrast Agent

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Unless otherwise noted, materials and solvents were obtained from commercial suppliers and used without further purification. All reactions were performed under an inert nitrogen atmosphere. EMD 60F 254 silica gel plates were used for thin layer chromatography and visualized using UV light or ninhydrin stain. Column chromatography was performed using standard grade 60 Å 230 – 400 mesh silica gel (Sorbent Technologies). 1H NMR and 13C NMR spectra were obtained at room temperature on a Bruker Avance III 500 MHz NMR spectrometer. An Agilent 6210 LC-TOF spectrometer was used to acquire electro spray ionization mass spectra (ESI-MS). Matrix-Assisted Laser Desorption Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry was carried out using a Bruker Autoflex III MALDI. Semi-preparative HPLC was performed on a Waters 19 × 250 mm X bridge C18 Column. Analytical HPLC was performed using a Waters 4.6 × 250 mm 5 μM X bridge C18 column using the Varian Prostar 500 system equipped with a Varian 363 fluorescence detector, and a Varian 335 UV/Vis Detector.
The amine functionalized Gd(III) scaffold (4) was synthesized according to literature procedure.30 For a detailed synthetic procedure of the peg functionalized scaffold (3) and the isothiocyanate functionalized dye (5) see the supporting information.
Harrison V.S., Carney C.E., MacRenaris K.W., Waters E.A, & Meade T.J. (2015). Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging. Journal of the American Chemical Society, 137(28), 9108-9116.
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7

One-dimensional 1H NMR Spectroscopy Protocol

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One-dimensional 1H NMR experiments were done with an Avance III 500 MHz NMR spectrometer using a 5 mm Prodigy TCI z-gradient (1H/19F/13C/15N) probe (Bruker Biospin Wissenbourg, France). Samples were analyzed on 5 mm tubes refrigerated at 6 °C on an auto-sampler (Sample Jet). NMR spectra were recorded at 25 °C K. A standard one-dimensional noe spectroscopy sequence (noesygppr1d with water presaturation and gradients) was used with low power irradiation of the water resonance during the recycle delay of 2 s and the mixing time of 10 ms. A total of 1024 scans was collected with a 90° impulsion time of 8.70 μs, acquisition time of 3.3 s, spectral window of 64 K data points zero-filled to 128 K before Fourier transformation with 0.3 Hz line broadening. All spectra were finally processed with Topspin version 3.5pl5 (Bruker Daltonics).
Wirgot N., Lagrée M., Traïkia M., Besaury L., Amato P., Canet I., Sancelme M., Jousse C., Diémé B., Lyan B, & Delort A.M. (2019). Metabolic modulations of Pseudomonas graminis in response to H2O2 in cloud water. Scientific Reports, 9, 12799.
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8

Synthesis of Schiff Base Ligand LH2

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6,6′-((1E,1′E)-((2,2-Dimethylpropane 1,3-diyl)bis(azanylylidene))bis(methanylylidene))bis(2-methoxyphenol) (LH2). To a stirring solution of o-vanillin (15 g, 98 mmol) in 200 mL of anhydrous MeOH under an inert atmosphere, 2,2-dimethyl-1,3-propanediamine (5.0 g, 49 mmol) in 30 mL of dry MeOH was added dropwise via a syringe. The reaction was stirred at room temperature for 24 h, producing a yellow/orange solution. The MeOH was removed in vacuo, and the resulting yellow residue was recrystallized from hot toluene to yield the product as yellow block-like crystals in 87% yield (Figure 1). Full 1H and 13C NMR spectra can be found in Figure S1. 1H NMR (500 MHz, chloroform-d) δ 14.12 (s, 2H), 8.29 (d, J = 1.4 Hz, 2H), 6.89 (dd, J = 7.8, 1.6 Hz, 2H), 6.85 (dd, J = 7.9, 1.5 Hz, 2H), 6.77 (t, J = 7.8 Hz, 2H), 3.88 (s, 6H), 3.46 (d, J = 1.2 Hz, 4H), 1.04 (s, 6H). 13C NMR (126 MHz, CDCl3) δ 165.80, 152.05, 148.47, 122.92, 118.40, 117.90, 113.81, 67.31, 56.03, 36.17, 24.25. 1H NMR and 13C NMR spectra were obtained at 25 °C on a Bruker Avance III 500 MHz NMR spectrometer.
Lilley L.M., Du K., Krzyaniak M.D., Parigi G., Luchinat C., Harris T.D, & Meade T.J. (2018). Effect of Magnetic Coupling on Water Proton Relaxivity in a Series of Transition Metal GdIII Complexes. Inorganic chemistry, 57(10), 5810-5819.
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9

31P NMR Analysis of Lipid Extracts

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The 31P NMR sample was prepared using a methanol reagent containing D2O and dissolved Cs-EDTA. Caesium salt EDTA was used due to the poor solubility of the sodium salt EDTA in organic solvents, and was prepared by titrating EDTA free acid into distilled water with solid CsOH until pH 6 was reached. The solution was then dried by lyophilization and dissolved in D2O to a concentration of 0.2 M. The methanol reagent was prepared by adding D2O-Cs-EDTA to methanol in the ratio of 1 to 4. For the 31P NMR sample, dried extract lipids were dissolved in 400 μL of deuterated chloroform and mixed with 200 μL of the methanol reagent containing Cs-EDTA. The sample was then placed in the NMR tube where two phases could be observed, a major chloroform phase and a smaller water phase. The 31P NMR spectra were recorded using a Bruker Avance III 500 MHz NMR spectrometer (Bruker, USA) equipped with a 5-mm CryoProbe Prodigy probehead. The experiment was performed at the Instrumentation Centre of National Taiwan University, Taipei, Taiwan.
Yeh V., Lee T.Y., Chen C.W., Kuo P.C., Shiue J., Chu L.K, & Yu T.Y. (2018). Highly Efficient Transfer of 7TM Membrane Protein from Native Membrane to Covalently Circularized Nanodisc. Scientific Reports, 8, 13501.
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10

NMR Characterization of Carbohydrate-Binding Domain

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NMR experiments were carried out at 303 K on a Bruker Avance III 600 MHz NMR spectrometer equipped with a TCI cryoprobe, and a Bruker Avance III 500 MHz NMR spectrometer. CRD chemical shift assignments were obtained from [13] (link) and two sets of heteronuclear NMR experiments (HNCA and HNCOCA, and HNCO and HNCACO) were performed on the 15N and 13C-labeled CRD sample at 400 mM concentration in the presence and the absence of Lactose. 1H-15N-HSQC titrations were performed on 15N-labeled proteins at 40 µM concentration for CRD and 30 µM for full length protein (FL), in the absence and the presence of various concentrations of ligands (Lactose or LNnT). All NMR experiments were performed on samples in 5 mM potassium phosphate buffer at pH 7.4. Lactose was purchased from Sigma and LNnT from Elicityl Company.
Halimi H., Rigato A., Byrne D., Ferracci G., Sebban-Kreuzer C., ElAntak L, & Guerlesquin F. (2014). Glycan Dependence of Galectin-3 Self-Association Properties. PLoS ONE, 9(11), e111836.
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