Plasma was collected in a stress-free environment by rapid non-terminal retro-orbital blood draw using thin hematocrit capillaries (Fisher Scientific). Aldosterone levels were determined on heparinized plasma (pre-diluted 2.5 times in PBS + 0.1% BSA) using an aldosterone I125 radioimmunoassay according to the manufacturer’s instructions (Tecan). Plasma renin activity was determined on EDTA-collected plasma using a competitive radioimmunoassay for Angiotensin I following the manufacturer’s instructions (Tecan). To rule out the impact of limiting amounts of angiotensinogen substrate in the plasma, an excess (about 180 ng) of angiotensinogen 1–14 renin substrate porcine (Sigma) was added to every sample, calibrator and control before quantification.
Competitive radioimmunoassay for angiotensin 1
Manufactured by Tecan
The Competitive radioimmunoassay for Angiotensin I is a laboratory equipment product designed for the quantitative determination of Angiotensin I in biological samples. It utilizes a competitive radioimmunoassay principle to measure the concentration of Angiotensin I.
Automatically generated - may contain errors
2 protocols using competitive radioimmunoassay for angiotensin 1
1
Aldosterone and Renin Activity Assay
2
Aldosterone and Renin Activity Assay
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