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Phoenix gp

Manufactured by American Type Culture Collection
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The Phoenix-GP is a compact, automated microbiology instrument designed for antimicrobial susceptibility testing. It utilizes fluorescence technology to rapidly detect and identify microorganisms, as well as determine their susceptibility to various antimicrobial agents. The Phoenix-GP is capable of performing a wide range of microbiological tests and provides reliable results in a timely manner.

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Lab products found in correlation

Hek293t, by American Type Culture Collection (4 mentions) Hek293t, by Corning (2 mentions) Bnl cl 2, by American Type Culture Collection (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Hepg2, by Corning (1 mentions) Sk hep1, by Corning (1 mentions) Sk hep1, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Mc38 cell line, by Kerafast (1 mentions) Recombinant human egf, by Thermo Fisher Scientific (1 mentions) Lncap fgc, by American Type Culture Collection (1 mentions) Du145, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Takara Bio (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Nk 92 cells, by American Type Culture Collection (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Recombinant interleukin 2 il 2, by R&D Systems (1 mentions) Mm 1s, by Thermo Fisher Scientific (1 mentions) Dmem glutamax, by Thermo Fisher Scientific (1 mentions) Rpmi8226, by American Type Culture Collection (1 mentions)

5 protocols using phoenix gp

1

Cell Culture Conditions Across Cell Lines

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HEK293T, Phoenix-GP, BNL CL.2, HepG2, and SK-HEP-1 cells were purchased from ATCC. All cells were negative for mycoplasm. BNL CL.2, DihXD3, HepG2, SK-HEP-1 and HEK293T cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine in an atmosphere of 95% air and 5% CO2.
Kudo Y., Sugimoto M., Arias E., Kasashima H., Cordes T., Linares J.F., Duran A., Nakanishi Y., Nakanishi N., L’Hermitte A., Campos A., Senni N., Rooslid T., Roberts L.R., Maria Cuervo A., Metallo C.M., Karin M., Diaz-Meco M.T, & Moscat J. (2020). PKCλ/ι loss induces autophagy, oxidative phosphorylation, and NRF2 to promote liver cancer progression. Cancer cell, 38(2), 247-262.e11.
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2

Cell Line and Organoid Culturing Protocols

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HEK293T and Phoenix-GP (sex: female) cell lines were purchased from ATCC. MC38 cell line (sex: female) was purchased from Kerafast. MODE-K cell line (sex: female) was kindly provided by Prof. Blumberg (Harvard Medical School, Boston, USA). Mouse tumor organoids (MTOs) were obtained from Dr. Eduard Batlle (Institute for Research in Biomedicine, Barcelona, Spain) and previously described (Tauriello et al., 2018 (link)). HEK293T, Phoenix GP, MODE-K and MC38 cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM, Corning) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine in an atmosphere of 95% air and 5% CO2. MTOs were cultured in advanced DMEM/F12 medium supplemented with 10 mM HEPES, Glutamax, B-27 (all Life Technologies), 50 ng/ml recombinant human EGF (Peprotech). Normal intestinal organoids were cultured in Advanced DMEM/F12 containing 10 mM HEPES, 1X Glutamax, 1X N2 supplement, 1X B27 supplement, 50 ng/ml EGF, 1000 ng/ml R-spondin 1, 100 ng/ml Noggin, and 10 μM Y-2763 in an atmosphere of 95% air and 5% CO2. Cultures were tested monthly for mycoplasma contamination.
Linares J.F., Zhang X., Martinez-Ordoñez A., Duran A., Kinoshita H., Kasashima H., Nakanishi N., Nakanishi Y., Carelli R., Cappelli L., Arias E., Yashiro M., Ohira M., Patel S., Inghirami G., Loda M., Cuervo A.M., Diaz-Meco M.T, & Moscat J. (2021). PKCλ/ι inhibition activates an ULK2-mediated interferon response to repress tumorigenesis. Molecular cell, 81(21), 4509-4526.e10.
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3

Cell Line Characterization and Authentication

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HEK293T (sex: female), C42B (sex: male), LNCaP-FGC (sex: male), PC3 (sex: male), DU145 (sex: male) and Phoenix-GP (sex: female) were obtained directly from ATCC. PrEC cell line (sex: male) was purchased from LONZA. All cells were negative for mycoplasm.
Reina-Campos M., Linares J.F., Duran A., Cordes T., L’Hermitte A., Badur M.G., Bhangoo M.S., Thorson P.K., Richards A., Rooslid T., Garcia-Olmo D.C., Nam-Cha S.Y., Salinas-Sanchez A.S., Eng K., Beltran H., Scott D.A., Metallo C.M., Moscat J, & Diaz-Meco M.T. (2019). Increased Serine and One Carbon Pathway Metabolism by PKCλ/ι Deficiency Promotes Neuroendocrine Prostate Cancer. Cancer cell, 35(3), 385-400.e9.
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4

Standardized Cell Line Maintenance and Transduction

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MCF7, T47D, HEK293T, and Phoenix-GP were purchased from ATCC. Unless otherwise indicated, the cell lines were maintained in RPMI supplemented with 10% fetal bovine serum (Takara Clontech, Heidelberg, Germany), 1% L-glutamine, and 1% penicillin/streptomycin. The packaging cell lines Phoenix-GP and HEK293T were used for the generation of retro-or lentiviruses following standard calcium phosphate protocols. Cells were selected with puromycin or neomycin. For all experiments, pooled transduced selected cell clones were used. Transduction efficiencies were monitored by flow cytometric detection of EGFP expression.
Roßwag S., Thiede G., Sleeman J.P, & Thaler S. (2020). RASSF1A Suppresses Estrogen-Dependent Breast Cancer Cell Growth through Inhibition of the Yes-Associated Protein 1 (YAP1), Inhibition of the Forkhead Box Protein M1 (FOXM1), and Activation of Forkhead Box Transcription Factor 3A (FOXO3A). Cancers, 12(9), 2689.
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5

Cell Culture Conditions for Various Cell Lines

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PG13 (ATCC CRL-10686), HEK293 (ATCC CRL-1573) and Phoe-nixGP (ATCC CRL-3215) cells were cultured in Dulbecco's Modified Eagle Medium+ Glutamax (DMEM-Glutamax) with the addition of 10% fetal bovine serum (FBS; Gibco, Billings, MT, USA). K562 (CCL-243), RPMI-8226 (ATCC CCL-155) and MM.1S (CRL-2974) were cultured in Roswell Park Memorial Institute (RPMI) 1640 media (Invitrogen, Carlsbad, CA, USA) enriched with 10% FBS. NK-92 cells (ATCC CSC-C0499) were maintained in Good Manufacturing Practice (GMP)-grade SCGM media (Sartorius CellGenix, Freiburg, Germany) supplemented with 20% FBS and 500 IU/mL recombinant interleukin-2 (IL-2; R&D Systems, Minneapolis, MN, USA) every 2À3 days. Cells were cultured in antibiotic-free conditions and were verified to be mycoplasma free with regular testing. The cells were maintained in a 37°C, 5% CO 2 humidified incubator and split every 2À3 days.
Karvouni M., Vidal-Manrique M., Susek K.H., Hussain A., Gilljam M., Zhang Y., Gray J.D., Lund J., Kaufmann G., Ljunggren H.G., Ji H., Lundqvist A., Wagner A.K., Guo W, & Alici E. (2023). Challenges in αCD38-chimeric antigen receptor (CAR)-expressing natural killer (NK) cell-based immunotherapy in multiple myeloma: Harnessing the CD38dim phenotype of cytokine-stimulated NK cells as a strategy to prevent fratricide. Cytotherapy, 25(7).
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