Melphalan hydrochloride

Manufactured by Merck Group

Sourced in United States

Melphalan hydrochloride is a pharmaceutical compound used as a laboratory reagent. It is a white to off-white crystalline powder that is soluble in water. Melphalan hydrochloride is commonly used in research applications, but its specific functions and intended uses should not be extrapolated upon in this response.

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Lab products found in correlation

Cisplatin, by Selleck Chemicals (1 mentions) Alpha amanitin, by Merck Group (1 mentions) Actinomycin d, by Merck Group (1 mentions) Hplc system, by Agilent Technologies (1 mentions) Milli q system, by Merck Group (1 mentions) Chemstation software, by Agilent Technologies (1 mentions)

3 protocols using melphalan hydrochloride

Melphalan Quantification in Human Plasma

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Melphalan hydrochloride was purchased from Sigma-Aldrich Co. (Saint
Louis, MO, USA); the internal standard (IS) N-acetyl melphalan (Ac-MPL) was from
ChemDiv Inc. (San Diego, CA, USA). Acetonitrile, methanol, water, and ammonium
acetate, acetic acid, trichloroacetic acid (Certified ACS) were purchased from
Thermo-Fisher Scientific (Fair Lawn, NJ, USA) as HPLC grade unless mentioned
individually. Human plasma (sodium heparin as the anticoagulant) was purchased
from Biological Specialty Co. (Colmar, PA, USA). Mobile phase A was prepared by
dissolving 1.927g ammonium acetate (NH₄AC) and 1mL glacial acetic
acid in 1L water followed by filtration before use; 20% trichloroacetic acid
(TCA) was prepared by dissolving 4g TCA in 20mL water.

Huang L., Cheah V., Chan D., Marzan F., Dvorak C.C., Aweeka F.T, & Long-Boyle J. (2019). Determination of melphalan in human plasma by UPLC-UV method. Cancer chemotherapy and pharmacology, 83(5), 905-910.

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Anticancer Drug Screening in Cell Lines

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Cell culture medium was replaced with medium containing indicated concentrations of drugs for indicated time periods. All drugs were dissolved in DMSO, and DMSO in the absence of inhibitor was used as a control in all experiments. Substances in the initial experiments were from the “approved oncology drugs set IV” library obtained from the National Cancer Institute (NCI), USA (https://wiki.nci.nih.gov/display/NCIDTPdata/Compound+Sets). The after substances were purchased: Melphalan hydrochloride (Y0001158, Sigma), Etiposide (S1225, Selleckchem), Cisplatin (S1166, Selleckchem), actinomycin D (A9415, Sigma), alpha‐amanitin (A2263, Sigma) and PARP1 inhibitor A‐966492 (S2197, Selleckchem).

Wu C., Nilsson K., Zheng Y., Ekenstierna C., Sugiyama N., Forslund O., Kajitani N., Yu H., Wennerberg J., Ekblad L, & Schwartz S. (2018). Short half‐life of HPV16 E6 and E7 mRNAs sensitizes HPV16‐positive tonsillar cancer cell line HN26 to DNA‐damaging drugs. International Journal of Cancer, 144(2), 297-310.

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HPLC Quantification of Melphalan

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Melphalan hydrochloride was purchased from Sigma-Aldrich. Stock solutions of melphalan were prepared in methanol and stored at -20°C to minimize degradation. Solvents were high-pressure liquid chromatography (HPLC) grade (Sintorgan). HPLC-grade water was obtained using a Milli-Q system (Millipore Corporation).
Melphalan concentrations were determined by HPLC coupled with a fluorescence detector according to a method previously developed and validated by our group. 19 Briefly, the analysis was performed with an Agilent HPLC system equipped with an Agilent 1100 liquid chromatography pump and an Agilent fluorescence detector set at an excitation/ emission wavelength of 270 and 350 nm, respectively. Separation chromatography was performed using a Nova-pack C18 reverse-phase column (150 • 3.9 mm i.d., 4 mm particle size; Waters) coupled to a C18 Phenomenex security guard precolumn. Data acquisition and processing were performed using the Agilent ChemStation software. The linear ranges for plasma and vitreous/aqueous humor assays were from 0.01 to 0.70 mg/mL and from 0.01 to 10.0 mg/mL, respectively. Interday precision was <11% for melphalan in methanolic extracts and vitreous except for the lowest concentration that had an interday precision of <16%.

Buitrago E., Winter U., Williams G., Asprea M., Chantada G, & Schaiquevich P. (2016). Pharmacokinetics of Melphalan After Intravitreal Injection in a Rabbit Model. Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 32(4).

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