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Ebio473p19

Manufactured by Thermo Fisher Scientific
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The EBio473P19 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for use in scientific research and analysis applications. The core function of this product is to provide users with a reliable and efficient tool for their laboratory tasks. A detailed description of the product's specifications and intended use is not available at this time.

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Lab products found in correlation

Bfa solution, by Thermo Fisher Scientific (2 mentions) Anti mouse igg horseradish peroxidase, by Santa Cruz Biotechnology (2 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Il 23, by Thermo Fisher Scientific (1 mentions) Muramyl dipeptide mdp, by Bachem (1 mentions) Lipid a, by Peptide Institute (1 mentions) Il 10, by BD (1 mentions) Pam3cys, by Merck Group (1 mentions)

3 protocols using ebio473p19

1

Macrophage Immune Response Profiling

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Monocyte-derived macrophages (MDMs) were treated with muramyl dipeptide (MDP; Bachem, Torrance, CA), lipid A (Peptides International, Louisville, KY), Pam3Cys (EMD Millipore, Billerica, MA), polyI:C, flagellin, CL097, CpG (InvivoGen, San Diego, CA), IL23 (PeproTech), 10:1 multiplicity of infection Salmonella enterica serovar Typhimurium or adherent invasive Escherichia coli (AIEC) strain LF82. In some cases cells were incubated with neutralising anti-IL23p19 antibody (eBioscience, San Diego, CA) for 1 hour before the treatments. Supernatants were assayed for tumour necrosis factor (TNF), IL6, IL8, IL10 (BD Biosciences, San Jose, CA), IL1β or IL23 (coating antibody to IL23p19, clone eBio473P19, detection antibody to IL12/ IL23p40, clone C8.6) (eBioscience) by ELISA.
Sun R., Hedl M, & Abraham C. (2019). IL23 induces IL23R recycling and amplifies innate receptor-induced signalling and cytokines in human macrophages, and the IBD-protective IL23R R381Q variant modulates these outcomes. Gut, 69(2), 264-273.
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2

Immunoblotting Analysis of Key Proteins

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Equal quantities of whole-cell lysates were resolved in SDS-polyacrylamide gel and immunoblotted with the following antibodies at their respective dilutions: anti-RUNX3 (1:1,500; R3-5G4), anti-RUNX1 (1:800; R1-1H9), anti-IL23A (1:250; eBio473P19, eBioscience), anti-α-tubulin (1:10,000; DM1A, Sigma-Aldrich), and anti-mouse IgG-horseradish peroxidase (1:5,000; Santa Cruz). To inhibit protein secretion, cells were treated with 1X BFA solution (eBioscience) 8 hr prior to harvest.
Hor Y.T., Voon D.C., Koo J.K., Wang H., Lau W.M., Ashktorab H., Chan S.L, & Ito Y. (2014). A Role for RUNX3 in Inflammation-Induced Expression of IL23A in Gastric Epithelial Cells. Cell reports, 8(1), 50-58.
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3

Immunoblotting Analysis of Key Proteins

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Equal quantities of whole-cell lysates were resolved in SDS-polyacrylamide gel and immunoblotted with the following antibodies at their respective dilutions: anti-RUNX3 (1:1,500; R3-5G4), anti-RUNX1 (1:800; R1-1H9), anti-IL23A (1:250; eBio473P19, eBioscience), anti-α-tubulin (1:10,000; DM1A, Sigma-Aldrich), and anti-mouse IgG-horseradish peroxidase (1:5,000; Santa Cruz). To inhibit protein secretion, cells were treated with 1X BFA solution (eBioscience) 8 hr prior to harvest.
Hor Y.T., Voon D.C., Koo J.K., Wang H., Lau W.M., Ashktorab H., Chan S.L, & Ito Y. (2014). A Role for RUNX3 in Inflammation-Induced Expression of IL23A in Gastric Epithelial Cells. Cell reports, 8(1), 50-58.
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