Rabbit anti pabp

Manufactured by Abcam

Rabbit anti-PABP is a primary antibody that recognizes the PABP (Poly(A) Binding Protein) protein. PABP is involved in the regulation of mRNA translation and stability.

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Lab products found in correlation

Mouse anti myc antibody, by Merck Group (1 mentions) Mouse anti puromycin, by Merck Group (1 mentions) Rabbit anti fak, by Thermo Fisher Scientific (1 mentions) Rabbit anti tubulin, by Abcam (1 mentions) Rabbit anti calnexin antibody, by Abcam (1 mentions) Rabbit anti tiar, by Cell Signaling Technology (1 mentions) Alexa fluor 488 tyramide superboost kit, by Thermo Fisher Scientific (1 mentions) Rabbit anti tdp 43, by Proteintech (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Goat anti eif3η, by Santa Cruz Biotechnology (1 mentions) Goat anti tia1, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Chicken anti gfp, by Abcam (1 mentions) Mouse anti g3bp1, by BD (1 mentions) Mowiol, by Merck Group (1 mentions) Mouse anti polyq, by Merck Group (1 mentions) Alexa fluor 568f ab 2 fragment of goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Mouse anti g3bp1, by Abcam (1 mentions)

3 protocols using rabbit anti pabp

Antibodies for ADAM15 Characterization

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Several antibodies directed against the extracellular part ADAM15 were used: a goat polyclonal (# AF935) and a mouse monoclonal (# mab945) from R&D Systems. Rabbit anti-ADAM15 (cytoplasmic domain, # ab84834), rabbit anti-PABP (# ab21060), rabbit anti-tubulin (# ab134185) and rabbit anti-calnexin antibody (# ab22595) from Abcam. Mouse anti-CD25 (# 174–820) from Ancell-Enzo Life Sciences GmbH. Mouse anti-puromycin (# MABE343, clone 12D10), mouse anti-myc antibody, (# 05–724), rabbit anti-alpha5 integrin (# AB1921) from Merck Millipore. Rabbit anti-FAK (# AHO0502) was from Invitrogen. Anti-Glutathion-S-Transferase peroxidase conjugate (# A7340) from Sigma-Aldrich.

Böhm B.B., Fehrl Y., Janczi T., Schneider N, & Burkhardt H. (2018). Cell adhesion-induced transient interaction of ADAM15 with poly(A) binding protein at the cell membrane colocalizes with mRNA translation. PLoS ONE, 13(9), e0203847.

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Immunofluorescence Analysis of Stress Granules

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Cells growing on glass-bottom dishes (ibidi; 81156) were fixed in 4% PFA for 5 min at room temperature, permeabilized with 0.1% TBST, blocked in 3% BSA, and then incubated with one of the following primary antibodies at 4°C overnigh t: mouse anti-FLAG (also recognizes the “DDK” tag) (Sigma Aldrich; F3165), mouse anti-G3BP1 (BD Biosciences; 611126), chicken anti-GFP (Abcam; ab13970), goat anti-TIA1 (Santa Cruz Biotechnology; sc-1751), rabbit anti-TIAR (Cell Signaling; 8509S), goat anti-eIF3η (Santa Cruz Biotechnology; sc-16377), rabbit anti–TDP-43 (Proteintech; 12892–1-AP), rabbit anti-PABP (Abcam; ab21060), rabbit anti-HA (Cell Signaling; 3724). Cells were then incubated with secondary antibodies conjugated to Alexa-488, −555 or −647 (Invitrogen), and mounted with ProLong Gold Antifade Reagent with DAPI (Invitrogen; P36931). To detect endogenous ULK1 and ULK2, the signal was amplified using Alexa Fluor 488 Tyramide SuperBoost Kit (Thermo Fisher Scientific; B40941) per the manufacturer’s instructions. Quantification of cells with stress granules was performed as previously described (Buchan et al., 2013 (link)).

Wang B., Maxwell B.A., Joo J.H., Gwon Y., Messing J., Mishra A., Shaw T.I., Ward A.L., Quan H., Sakurada S.M., Pruett-Miller S.M., Bertorini T., Vogel P., Kim H.J., Peng J., Taylor J.P, & Kundu M. (2019). ULK1/2 Regulates Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97. Molecular cell, 74(4), 742-757.e8.

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Immunofluorescence Staining of Polyglutamine Proteins

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Cells were fixed with paraformaldehyde (4% in PBS) for 20 min, followed by permeabilization (0.2% Triton X-100 in PBS, 10 min) and blocking (3% bovine serum albumin in 0.2% Triton X-100 in PBS, 10 min). Cells were incubated in primary antibody for 2 h at room temperature (Mouse anti-polyQ (Millipore, #MAB1574, 1:50), Rabbit anti-HTT (Cell Signaling, #5656, 1:200), Rabbit anti-G3BP1 (MBL, #RN048PW, 1:500), Mouse anti-G3BP1 (Abcam, #56574, 1:500), Rabbit anti-PABP (Abcam, #21060, 1:400)). Then, the cells were washed with 0.2% Triton-X/PBS and incubated with secondary antibody (Alexa Fluor 488 Goat anti-Mouse (ThermoFisher Scientific, #A11029, 1:500), Alexa Fluor 568F(ab′)2 Fragment of Goat Anti-Rabbit IgG (H + L) (ThermoFisher Scientific, #A-21069, 1:500), or Alexa Fluor 594 Goat Anti-Rabbit IgG (H + L) (ThermoFisher Scientific, #A-11037, 1:500)) and Hoechst 33342 (Life Technologies, #H3570, 1:1000) for 1 h at room temperature. PBS and distilled water washes were followed and then the cover slips were mounted on Mowiol (Sigma, #324590).

Gutiérrez-Garcia R., Koyuncu S., Hommen F., Bilican S., Lee H.J., Fatima A, & Vilchez D. (2023). G3BP1-dependent mechanism suppressing protein aggregation in Huntington’s models and its demise upon stress granule assembly. Human Molecular Genetics, 32(10), 1607-1621.

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