TEM images were obtained at the UC Berkeley Electron Microscope Lab (www.em-lab.berkeley.edu ) using a FEI Tecnai 12 transmission electron microscope with 120 kV accelerating voltage. Protein samples were prepared for TEM analysis by pipetting 5 µL of the samples onto Formvar-coated copper mesh grids (400 mesh, Ted Pella, Redding, CA), after 3 min of equilibration the samples were then wicked with filter paper. The samples were then rinsed with dd-H2O. Subsequently, the grids were exposed to 8 µL of a 1% (w/v) aqueous solution of uranyl acetate for 90 s as a negative stain. After excess stain was removed, the grids were allowed to dry in air.
Formvar coated copper mesh grids
Manufactured by Ted Pella
Sourced in United States
Formvar-coated copper mesh grids are a type of laboratory equipment used in electron microscopy. They provide a stable and uniform support structure for thin samples to be examined under an electron beam. The Formvar coating serves as a thin, transparent film that helps to suspend and stabilize the sample during the imaging process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using formvar coated copper mesh grids
1
Protein Imaging with Negative Staining TEM
2
Negative Staining of Virus-Like Particles
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Five microliters
of VLP-based samples were pipetted onto Formvar-coated copper mesh
grids (400 mesh, Ted Pella, Redding, CA, USA) for 5 min, followed
by exposure to 8 μL of a solution of uranyl acetate (15 mg/mL
in DI-H2O) for 2 min as a negative stain. Excess stain
was then removed, and the grids could dry in air for 10 min.
of VLP-based samples were pipetted onto Formvar-coated copper mesh
grids (400 mesh, Ted Pella, Redding, CA, USA) for 5 min, followed
by exposure to 8 μL of a solution of uranyl acetate (15 mg/mL
in DI-H2O) for 2 min as a negative stain. Excess stain
was then removed, and the grids could dry in air for 10 min.
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