Te2000e widefield microscope

Manufactured by Nikon

The TE2000E widefield microscope is a laboratory instrument designed for magnified viewing and imaging of samples. It features a modular design with interchangeable components, allowing for customization to suit various applications. The microscope's core function is to provide high-quality, detailed visual representations of specimens under examination.

Automatically generated - may contain errors

Lab products found in correlation

Elements advanced research software, by Nikon (2 mentions) Plan apo vc, by Nikon (2 mentions) Elements advanced research imaging software, by Nikon (1 mentions) Plan apo 1.4 na, by Nikon (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Nis elements advanced research imaging software, by Nikon (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Widefield microscope

4 protocols using te2000e widefield microscope

Standardized Blue Light Exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The amount of blue light delivered to the cells was normalized throughout all experiments using a power meter to measure the amount of light output at the 20× and 60× objectives of widefield and confocal microscopes, respectively. On the Nikon TE2000E widefield microscope using a 20× with a 0.75 numerical aperture (NA) lens and an FITC HyQ filter from chroma, blue light output was measured at approximately 12.8 microwatts (μW) at the objective. The Nikon A1R confocal microscope at 4% of total output power of the 488 nm laser delivered 12.5 to 13 μW of light through a 60× Plan Apo 1.4 NA objective lens. Nikon Elements Advanced Research imaging software (Nikon instruments) was used for the automated exposure of blue-light pulses to the cells. To administer the blue light stimulation to cells using the widefield microscope, a 30-s pulse was delivered to the entire plate. In live-imaging experiments such as uptake, the 488-nm laser was set to 4% of total power output to deliver a 30-s pulse to the cells before initiating the experiment.

Ingram S.M., Rana T., Manson A.M., Yayah F.M., Jackson E.G., Anderson C., Davids B.O, & Goodwin J.S. (2021). Optogenetically-induced multimerization of the dopamine transporter increases uptake and trafficking to the plasma membrane. The Journal of Biological Chemistry, 296, 100787.

+ Open protocol

+ Expand

Quantifying Mitochondrial Morphology in T. brucei

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live T. brucei cells (5 × 10⁶) expressing TbTim17 mutants were used for MitoTracker staining as previously described (69 (link)). Briefly, cells were washed twice with PBS and spread evenly over gelatin-coated slides. Once the cells had settled, the slides were washed with cold PBS to remove any unattached cells. The attached cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.1% Triton X-100. After blocking with 5% non-fat milk for 30 min, the slides were washed with 1× PBS. DNA was stained with 1 µg/mL 4′,6-diamidino-2-phenylindole. Cells were imaged using a Nikon TE2000E widefield microscope equipped with a 60 × 1.4 NA Plan Apo VC oil immersion objective. Images were captured using a CoolSNAP HQ2 cooled CCD camera and Nikon Elements Advanced Research Software. There were no max projections of a Z-stack. The software used to calculate the Pearson’s coefficient is Nikon NIS Elements Advanced Research Imaging Software.

Darden C., Donkor J.E., Korolkova O., Barozai M.Y, & Chaudhuri M. (2024). Distinct structural motifs are necessary for targeting and import of Tim17 in Trypanosoma brucei mitochondrion. mSphere, 9(1), e00558-23.

+ Open protocol

+ Expand

Imaging of T. brucei Mitochondrial Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live T. brucei cells (5 × 10⁶) expressing TbTRAP1–3xHA and 6xMyc-TbTim17 (in situ tagged) were used for MitoTracker staining as previously described (26 (link)). Briefly, cells were washed twice with PBS and spread evenly over poly-L-lysine (100 μg/ml)–coated slides. Once the cells had settled, the slides were washed with cold PBS to remove any unattached cells. The attached cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.1% Triton X-100. After blocking with 5% nonfat milk for 30 min, the slides were washed with 1× PBS. Monoclonal anti-Myc antibody and polyclonal anti-HA were used as the primary antibody; FITC-conjugated antimouse IgG and Alexa Fluor 594–conjugated anti-rabbit were used as the secondary antibodies, respectively, for visualization under a fluorescent microscope. DNA was stained with 1 μg/ml 4′,6-diamidino-2-phenylindole. Cells were imaged using a Nikon TE2000E widefield microscope equipped with a 60 × 1.4 NA Plan Apo VC oil immersion objective. Images were captured using a CoolSNAP HQ2 cooled CCD camera and the Nikon Elements Advanced Research software.

Soto-Gonzalez F., Tripathi A., Cooley A., Paromov V., Rana T, & Chaudhuri M. (2022). A novel connection between Trypanosoma brucei mitochondrial proteins TbTim17 and TbTRAP1 is discovered using Biotinylation Identification (BioID). The Journal of Biological Chemistry, 298(12), 102647.

+ Open protocol

+ Expand

Live T. brucei Mitochondrial Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Live T. brucei cells (5×10⁶) expressing TbTim54–12X-myc (in situ-tagged) were used for MitoTracker staining as previously described [Singha et al., 2008 (link)]. Briefly, MitoTracker® Red CMXROS (Molecular Probe®) in DMSO (1 mM) was added to cells in culture medium to a final concentration of 0.5 μM. The mixture was incubated at 37 °C for 10 min. Cells were washed and incubated in fresh culture medium for an additional 30 min. Cells were then washed twice with PBS and spread evenly over poly-L-lysine (100 μg/ml in H₂O)-coated slides. Once the cells had settled, the slides were washed with cold PBS to remove any unattached cells. The attached cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.1% Triton X-100. After blocking with 5% non-fat milk for 30 min, the slides were washed with 1X PBS. Monoclonal anti-Myc antibody was used as the primary antibody and a fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG was used as a secondary antibody for visualization under a fluorescent microscope. DNA was stained with 1μg/ml 4′,6-diamidino-2-phenylindole (DAPI). Cells were imaged using a Nikon TE2000E widefield microscope equipped with a 60× 1.4 NA Plan Apo VC oil immersion objective. Images were captured using a CoolSNAP HQ2 cooled CCD camera and the Nikon Elements Advanced Research software.

Singha U.K., Tripathi A., Smith JT J.r., Quinones L., Saha A., Singha T, & Chaudhuri M. (2020). Novel IM-associated protein Tim54 plays a role in the mitochondrial import of internal signal-containing proteins in Trypanosoma brucei. Biology of the cell, 113(1), 39-57.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!