Each sample was inserted into the HPLC system (Nexera-i LC-2040C plus, Shimadzu Co., Kyoto, Japan), with a volume of 20 μL for and ISMN, or 10 μL for the other chemicals besides MP. The YMC-Triart C18 column (4.6 mm × 250 mm, 5.0 μm; YMC Co., Ltd., Kyoto, Japan) was maintained at 40 °C. The flow rate was 1.0 mL/min. The conditions for the mobile phase and absorption wavelength of each target were as follows: 0.1% (w/v) phosphoric acid (acetonitrile = 95:5, 270 nm for KA; acetonitrile = 80:20, 270 nm for CAF; acetonitrile = 85:15, 230 nm for ISMN; acetonitrile = 70:30, 220 nm for LID; acetonitrile = 80:20, 230 nm for BA). Additionally, for MP and p-hydroxy benzoate (PHBA), which is the metabolite of MP, the insertion volume was 3.0 μL. The YMC-Triart C18 column (2.1 mm × 100 mm, 1.9 μm; YMC Co., Ltd.) was also maintained at 40 °C. The flow rate was 0.15 mL/min. The conditions for the mobile phase and absorption wavelength were as follows: 0.1% (w/v) phosphoric acid (acetonitrile = 85:15, 255 nm).
Triart c18 column
Manufactured by YMC
Sourced in Japan, United States, Germany
The YMC-Triart C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of organic compounds. The column features a stationary phase composed of silica-based particles chemically modified with C18 (octadecyl) functional groups, which provide effective retention and separation of both polar and non-polar analytes. The column's technical specifications, such as particle size, pore size, and column dimensions, can vary depending on the specific application requirements.
Automatically generated - may contain errors
Lab products found in correlation
Kieselgel 60 f254, by Merck Group (3 mentions)
Jnm eca 500 mhz nmr instrument, by JEOL (3 mentions)
Sil 20a, by Shimadzu (2 mentions)
Dgu 20a3r, by Shimadzu (2 mentions)
Spd m20a, by Shimadzu (2 mentions)
Maxis ultrahigh resolution tof system, by Bruker (2 mentions)
Vwd 3400 detector, by Thermo Fisher Scientific (2 mentions)
Compass hystar software package, by Bruker (2 mentions)
Ultimate 3000 uhplc system, by Thermo Fisher Scientific (2 mentions)
High performance liquid chromatography (hplc), by Waters Corporation (2 mentions)
High performance liquid chromatography (hplc), by Shimadzu (2 mentions)
1200 series lc system, by Agilent Technologies (2 mentions)
Nexera x2, by Shimadzu (2 mentions)
11c r pk11195, by Horiba (2 mentions)
Xterra c18 column, by Waters Corporation (2 mentions)
Lc 8a pump, by Shimadzu (2 mentions)
Proteinpilot, by AB Sciex (1 mentions)
Peakview, by AB Sciex (1 mentions)
Tripletof 6600, by AB Sciex (1 mentions)
Cto 20a, by Shimadzu (1 mentions)
91 protocols using triart c18 column
1
HPLC Analysis of Pharmaceutical Compounds
2
Phosphopeptide Analysis by microUHPLC-ESI-QTOF
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Phosphopeptides
were analyzed by microUHPLC (Ultimate 3200 RS, ThermoFisher Scientific,
Idstein, Germany) interfaced with an ESI–QTOF mass spectrometer
(6600 TripleTOF, AB Sciex, Darmstadt, Germany). A Triart C18 column
(100 × 0.5 mm, 3 μm; YMC Europe, Dinslaken, Germany) equipped
with a precolumn containing the same material, operating with a flow
rate of 30 μL/min and 35 °C column temperature was chosen
for chromatographic separation. The mobile phase consisted of 0.1%
formic solution (eluent A) and acetonitrile containing 0.1% formic
acid (eluent B) and the separation was conducted applying the gradient
15.0 min 2% B, 5.0 min 2% B, 55.0 min 42.5% B, 55.5 min 95% B, and
65.0 min 95% B. The sample volume was 4 μL. Ion spray voltage
was set to 5200 V and declustering potential to 80 V. Nitrogen was
used as the collision gas in the MS/MS experiments for peptide sequencing.
Raw data were processed using PeakView (version 2.2, AB Sciex, Darmstadt,
Germany) and Protein Pilot (version 5.0, AB Sciex, Darmstadt, Germany).
were analyzed by microUHPLC (Ultimate 3200 RS, ThermoFisher Scientific,
Idstein, Germany) interfaced with an ESI–QTOF mass spectrometer
(6600 TripleTOF, AB Sciex, Darmstadt, Germany). A Triart C18 column
(100 × 0.5 mm, 3 μm; YMC Europe, Dinslaken, Germany) equipped
with a precolumn containing the same material, operating with a flow
rate of 30 μL/min and 35 °C column temperature was chosen
for chromatographic separation. The mobile phase consisted of 0.1%
formic solution (eluent A) and acetonitrile containing 0.1% formic
acid (eluent B) and the separation was conducted applying the gradient
15.0 min 2% B, 5.0 min 2% B, 55.0 min 42.5% B, 55.5 min 95% B, and
65.0 min 95% B. The sample volume was 4 μL. Ion spray voltage
was set to 5200 V and declustering potential to 80 V. Nitrogen was
used as the collision gas in the MS/MS experiments for peptide sequencing.
Raw data were processed using PeakView (version 2.2, AB Sciex, Darmstadt,
Germany) and Protein Pilot (version 5.0, AB Sciex, Darmstadt, Germany).
3
HPLC Analysis of Compounds
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The HPLC analysis was conducted with a Shimadzu LC-20A Prominence Series system (Shimadzu Corporation, Kyoto, Japan) equipped with a quaternary pump (LC-20AD), vacuum degasser (DGU-20A3R), autosampler (SIL-20A), column oven (CTO-20A), and photodiode-array detector (SPD-M20A). The chromatographic data were interpreted using LabSolutions Multi-PDA software. Chromatographic separation was performed on a YMC Triart C18 column (4.6 × 250 mm i.d., 5 μm). The column oven was maintained at 40°C, the detection was conducted at λ = 207 nm, and online UV absorption spectra were recorded in the range of 190 to 400 nm. The gradient elution was performed under the following conditions: initial mobile phase, acetonitrile/0.1% trifluoroacetic acid in water = 15 : 75 (v/v), 0∼15 min; 15 : 75 to 35 : 65 (v/v), 0∼10 min; 35 : 75 to 50 : 50 (v/v), 10∼20 min; 50 : 50 to 100 : 0 (v/v), 20∼25 min; and 100 : 0 (v/v), 25∼30 min. The flow rate was 1.0 mL/min, and the injection volume was 5 μL.
4
Enzymatic Reaction Analysis using HPLC
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The analysis of enzymatic reactions was performed on an Infinity II 1260 liquid chromatography system (Agilent). Separation of the enzymatic reaction products occurred on a Triart C18 column (YMC) in 30 mM ammonium acetate buffer system (pH 4.3) in a linear gradient of acetonitrile from 0 to 10% for 25 min. Processing of chromatograms was performed in OpenLab CDS ChemStation (Agilent). The elution profiles were exported in comma-separated value (.csv) format for visualization.
5
HPLC Analysis of Polyphenolic Compounds
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The polyphenolic composition in the DE was analyzed via high-performance liquid chromatography (Waters Scientific Ltd., Mississauga, ON, Canada) using a YMC-Triart C18 column (250 × 4.6 mm, 5 μm, YMC, Kyoto, Japan). The mobile phase was water and acetonitrile containing 0.2% formic acid, the flow rate was 0.8 mL/min, and the injection volume was 10 μL. The measurement wavelength was 260 nm for gallic acid, 3,4-dihydroxybenzoic acid, and rutin; 310 nm for chlorogenic acid, p-coumaric acid, caffeic acid, trans-ferulic acid, and apigenin; and 365 nm for quercetin and kaempferol [21 (link)].
6
Ginseng Extract Standardization and Characterization
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Standardized WG and RG, manufactured from a 5-year-old ginseng sample, were obtained from Gwangmyung Natural Pharmaceutical Co. (Busan, Korea), and voucher specimens (No. 202KWG and 202KRG) were deposited at the Herbarium of Pusan National University. For the preparation of both WG and RG extracts, 200 g of each sample was roughly ground and extracted with 10 volumes of methanol at 25°C for 24 h; this step was repeated thrice. After filtration, methanol was removed using a vacuum evaporator (Eyela, Japan) at 45°C, and the filtrate was stored at −20°C until further use. The final lyophilized WG extract (WGex) and RG extract (RGex) from WG and RG weighed 24.3 g and 25.2 g, yielding 12.2% and 12.6%, respectively.
For confirming the quality of WGex and RGex, each extract was subjected to HPLC analysis. To obtain a fingerprint of the extracts used in this experiment, we determined the presence of ginsenoside Rg1, a component of ginseng, using an HPLC system (Shimadzu, Kyoto, Japan) equipped with an LC-20AD pump, a SIL-20A sampler, an SPD-M20A detector, and a CTO-20A column oven. The samples were separated using a YMC Triart C18 column. Details of the mobile phase and elution system are listed inSupplementary Table 1 (Table S1 ).
For confirming the quality of WGex and RGex, each extract was subjected to HPLC analysis. To obtain a fingerprint of the extracts used in this experiment, we determined the presence of ginsenoside Rg1, a component of ginseng, using an HPLC system (Shimadzu, Kyoto, Japan) equipped with an LC-20AD pump, a SIL-20A sampler, an SPD-M20A detector, and a CTO-20A column oven. The samples were separated using a YMC Triart C18 column. Details of the mobile phase and elution system are listed in
7
High-Resolution Q-TOF Mass Spectrometry Analysis of Statin Derivatives
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High-resolution Q-TOF mass spectrometry analysis was carried out with a maXis ultrahigh-resolution TOF system (Bruker Daltonik, Germany). The reaction samples were analyzed by HPLC/HRMS with a Triart C18 column (YMC Co., Ltd., Japan). Mevastatin, pravastatin, YC-17, and their derivatives were monitored at 230 nm using a linear mobile phase gradient ranging from 20% (v/v) acetonitrile to 70% (v/v) acetonitrile in 0.1% (v/v) TFA aqueous solution over 30 min. CsA and CsA-4-OH were monitored at 210 nm and analyzed in a two-buffer gradient system consisting of 25% methanol (buffer A) and 100% acetonitrile (buffer B). The gradient elution profile was as follows: 0–4 min, 40% solvent B; 4–15 min, 40–61% solvent B; 15–32 min, 61–100% solvent B; 32–35 min, 40% solvent B. The flow rate was set at 1 mL/min, and the injection volume was 20 μL.
8
Quantitative HPLC Analysis of Amino Acids
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Experiments were performed on an Agilent 1200 HPLC system (Waldbronn, Germany), using a Triart C-18 column (150 × 3.00 mm, 3 μm) from YMC (Dinslaken, Germany). The mobile phase consisted of 10 mM ammonium acetate in water (A) and 10 mM ammonium acetate in acetonitrile (B). The components were eluted by using the following solvent gradient: 0–16 min 2% B, 16–25 min 2–16% B, 25–35 min 16–98% B. Then the column was re-equilibrated for 20 min with 2% B. The DAD was set to 260 nm except for tyrosine (2) and phenylalanine (4), which both were monitored at 210 nm. Flow rate, injected sample volume and column temperature were adjusted to 0.3 ml/min, 10 μl and 20 °C, respectively.
9
GABA Extraction and Quantification from Lettuce
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GABA was extracted from 50 mg of ground lettuce leaf in 100 µL water and subsequently sonicated for 10 min. Samples were then centrifuged at 13,000× g for 5 min. 45 µL of supernatant was diluted with 30 µL of a 25 mM NorValine solution (used as internal standard). GABA was then injected on a reverse HPLC system (Shimadzu, Kyoto, Japan) and derivatized before column injection as O-Phtalaldehyde and separated on a YMC Triart C18 column with the use of buffer “A” (50 mM KH2PO4 pH 6.5, 0.7% v/v Tetrahydrofurane) and buffer “B” (Acetonitrile:MeOH:water 45:40:15) with the following gradient: from 0 to 6 min = 96% A 4% B; from 6 to 18 min = 92% A 8% B; from 18 to 32 min = 85% A 15% B; from 32 to 50 min = 67% A 33% B; from 50 to 53 min = 100% B. GABA was detected with a fluorescence detector at λex = 230 nm and λem = 450 nm.
10
NMR and Mass Spectrometry Protocol
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Bruker AM500 spectrometer (Karlsruhe, Germany) used for measurement of proton and carbon-13 NMR spectra. By mass spectrometer JEOL JMS-700 (Tokyo, Japan) obtained all mass data. Forte/R 100 (YMC, Kyoto, Japan) recycling HPLC and MPLC with Triart C18 column (YMC, Japan) used for isolation of compounds. Methanol, acetonitrile, water and acetic acid of the analytical grade for HPLC were purchased from Fisher Scientific (Korea). For performing enzyme assays SpectraMax M3 Multi-Mode Microplate Reader (Molecular device, USA) was used. HNE (EC 3.4.21.37) was ordered from Sigma Aldrich (St. Louis, USA).
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