Nanodrop model nd 1000

Manufactured by Thermo Fisher Scientific

Sourced in United States

The NanoDrop model ND-1000 is a UV-Vis spectrophotometer designed for the quantification and analysis of small sample volumes. It utilizes a patented sample retention system that requires only 1-2 microliters of sample to perform measurements. The core function of the NanoDrop ND-1000 is to provide accurate and reproducible absorbance measurements in the 220-750 nm wavelength range.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy plant mini kit, by Qiagen (2 mentions) Abi 7900ht sequence detection system, by Thermo Fisher Scientific (1 mentions) Abi taqman allelic discrimination kit, by Thermo Fisher Scientific (1 mentions) Hiseq 2000 instrument, by Illumina (1 mentions) Genome analyzer 2, by Illumina (1 mentions) Bioanalyzer, by Agilent Technologies (1 mentions) Phosphate buffered saline (pbs), by HiMedia (1 mentions) Bhi broth, by HiMedia (1 mentions) Experion bioanalyzer, by Bio-Rad (1 mentions)

Spelling variants of this product

NanoDrop (8392 citations) NanoDrop 1000 (2999 citations) NanoDrop ND-1000 (2953 citations) ND-1000 (283 citations) Nanodrop model 1000 (2 citations) ND-1000 model NanoDrop Spectrophotometer (2 citations)

4 protocols using nanodrop model nd 1000

Genetic Profiling of SCARB1 and IL28B Variants

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three SNPs (rs10846744, rs5888, and rs3782287) of the SCARB1 gene with an allele minor frequency of >1% and have been linked to humans diseases were investigated13 (link)16 (link)17 (link)18 (link)19 (link).
All enrolled subjects were genotyped for the SNPs of the SCARB1 gene (rs10846744, rs5888, and rs3782287) and IL28B gene (rs8099917) by using the ABI TaqMan allelic discrimination kit and the ABI7900HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA)43 (link). All their blood specimens were collected into EDTA tubes, and human genomic DNA was extracted by standard protocols, including blood RBC lysis, cell lysis, DNA binding, wash, and elution. Extracted DNA was normalized to 50 ng/μl, and assessed by calculating the absorbance ratio OD260 nm/280 nm using NanoDrop model ND-1000 (Thermo Scientific, Wilmington, DE, USA).

Hsu C.S., Hsu S.J., Liu W.L., Chen D.S, & Kao J.H. (2016). Association of SCARB1 Gene Polymorphisms with Virological Response in Chronic Hepatitis C Patients Receiving Pegylated Interferon plus Ribavirin Therapy. Scientific Reports, 6, 32303.

+ Open protocol

+ Expand

RNA Extraction and Illumina Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols

All RNA extractions were performed with the Qiagen RNeasy Plant Mini kit (Cat# 74904), according to the manufacturer’s protocol. Total RNA was eluted twice with 30 μL nuclease-free water. The RNA samples were quantified by Nanodrop (model ND-1000, Thermo Scientific, Wilmington, DE). 1 μg total RNA from each line was used to prepare indexed RNA libraries using the Illumina protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part# 15008136 Rev. A, November 2010). Indexed libraries were quality checked via Bioanalyzer (Agilent Technologies, Santa Clara, CA) before sequencing. Shoot apex libraries were sequenced using an Illumina Genome Analyzer II with 76 cycles of single-end reads. All other libraries were sequenced using an Illumina Hi-seq 2000 instrument with 110 cycles of chemistry and imaging, resulting in paired-end (PE) sequencing reads with length of 2×101 bp.

Kusmec A., Srinivasan S., Nettleton D, & Schnable P.S. (2017). Distinct Genetic Architectures for Phenotype Means and Plasticities in Zea mays. Nature plants, 3(9), 715-723.

+ Open protocol

+ Expand

Bacterial DNA Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All isolates were grown anaerobically in BHI broth (HiMedia, Mumbai, India) overnight at 37 °C. Next, 1 mL of the bacterial suspension was mixed with the same volume of phosphate buffered saline (PBS, HiMedia, Mumbai, India) and centrifuged at 6000× g for 5 min. After the removal of supernatant, the microbial sediment was subjected to DNA extraction using a SinaClon bacterial Gram-positive DNA extraction kit (SinaClon Co., Tehran, Iran) according to the manufacturer’s instruction. The quality and quantity of the extracted genomes were evaluated spectrophotometrically using a NanoDrop model ND-1000 (ThermoFisher Scientific, Waltham, MA, USA). Prior to the PCR reactions, the concentrations of all extracted DNA were adjusted to 50 μg/mL with PBS.

Hassani S., Pakbin B., Brück W.M., Mahmoudi R, & Mousavi S. (2022). Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran. Antibiotics, 11(3), 340.

+ Open protocol

+ Expand

RNA Extraction and Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from each sample, using the CTAB-LiCl precipitation method [82 (link)], and purified with the RNeasy Plant Mini Kit (Qiagen, CA, USA). Quantity of total RNA for each sample was measured with Nanodrop model ND-1000 (Thermo Scientific, MA, USA), and RNA quality was checked using Experion Bioanalyzer (Bio-Rad, CA, USA).

Chano V., Collada C, & Soto A. (2017). Transcriptomic analysis of wound xylem formation in Pinus canariensis. BMC Plant Biology, 17, 234.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!