T udca

Manufactured by Steraloids

Sourced in United States

T-UDCA is a chemical compound used in laboratory research and analysis. It is a type of bile acid derivative. The core function of T-UDCA is to serve as a research tool for scientific investigations, but no further details about its intended use can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

T hdca, by Steraloids (5 mentions) Tcdca, by Merck Group (4 mentions) T β mca, by Steraloids (4 mentions) G lca, by Steraloids (3 mentions) G udca, by Steraloids (3 mentions) G dca, by Steraloids (3 mentions) T lca, by Steraloids (3 mentions) Gcdca, by Merck Group (2 mentions) Tβmca, by Merck Group (2 mentions) Tαmca, by Steraloids (2 mentions) T dca, by Steraloids (2 mentions) Propranolol, by Merck Group (1 mentions) Dehydrocholic acid, by Merck Group (1 mentions) Lithocholic acid lca, by Merck Group (1 mentions) Anti cyp7a1, by Santa Cruz Biotechnology (1 mentions) Anti pparα antibody, by Abcam (1 mentions) Anti fxr, by Santa Cruz Biotechnology (1 mentions) Hplc grade methanol, by Thermo Fisher Scientific (1 mentions) Anti gapdh antibody, by Bioworld Technology (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions)

5 protocols using t udca

Inflammatory Bowel Disease Biomarker Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

OEA was purchased from Santa Cruz Biotechnology (Santa Cruz, CA); DSS (molecular weight=36,000–50,000 kDa) was obtained from MP Biochemical (Santa Ana, CA). MPA O-glucuronide and naloxone 3-β-D-glucuronide were obtained from Toronto Research Chemicals Inc. (ON, Canada). β-MCA, UDCA, HDCA, G-LCA, G-UDCA, G-DCA, T-β-MCA, T-UDCA, T-HDCA and T-LCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, lithocholic acid (LCA), G-CDCA, G-CA, T-CDCA, T-DCA, T-CA, dehydrocholic acid and propranolol were purchased from Sigma-Aldrich (St Louis, MO). The anti-UGT1A, anti-FXR, anti-FGF15 and anti-CYP7A1 antibodies were purchased from Santa Cruz Biotechnology. The anti-PPARα antibody was from Abcam (Cambridge, UK). The anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from SunShine Biotechnology (Nanjing, China). The secondary antibodies and recombinant human FGF19 was obtained from Bioworld Technology (St Louis Park, MN). Other reagents, unless mentioned, were obtained from Sigma-Aldrich. RNA extracts of colon biopsies from 8 healthy humans and 13 ulcerative colitis and Crohn’s disease patients were previously described51 (link). The ulcerative colitis and Crohn’s disease samples were collected from consented patients and approved by the University of Michigan IRB committee (approval number HUM00042210).

Zhou X., Cao L., Jiang C., Xie Y., Cheng X., Krausz K.W., Qi Y., Sun L., Shah Y.M., Gonzalez F.J., Wang G, & Hao H. (2014). PPARα-UGT axis activation represses intestinal FXR-FGF15 feedback signalling and exacerbates experimental colitis. Nature Communications, 5, 4573.

+ Open protocol

+ Expand

Comprehensive Bile Acid Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade methanol and acetonitrile were purchased from Fisher Scientific (Nepean, Ont., Canada). Ultrapure water was prepared by the Milli-Q Ultrapure water purification system (Millipore, Bedford, MA, USA). AOM was obtained from Sigma-Aldrich (St. Louis, MO, USA), and DSS (MW 36000-50000) was purchased from MP Biomedicals (Santa Ana, CA). Ammonium acetate, formic acid, glacial acetic acid and the other reagents (analytical grade) were purchased from Nanjing Chemical Factory (Nanjing, China). ‘Mouse Total Bile Acids Kit’ was purchased from Crystal Chem Inc (Downers Grove, United States). ‘MiniBEST Bacterial Genomic DNA Extraction Kit’ was from TaKaRa Bio Inc (Dalian, China). Anti-FXR, FGF15 and CYP7A1 antibodies were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti-GAPDH antibody was from Bioworld Technology (St. Louis Park, MN, USA).
CA, CDCA, DCA, and LCA, as well as their glycine [38 (link)] and taurine [45 (link)] conjugates, G-CA, G-CDCA, T-β-MCA, T-CA, T-CDCA, T-DCA, and internal standard dehydrocholic acid (dhCA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). α-MCA, UDCA, HDCA, G-LCA, G-UDCA, G-DCA, T-β-MCA, T-UDCA, T-HDCA and T-LCA were purchased from Steraloids, Inc. (Newport, Rhode Island, USA).

Cao L., Che Y., Meng T., Deng S., Zhang J., Zhao M., Xu W., Wang D., Pu Z., Wang G, & Hao H. (2017). Repression of intestinal transporters and FXR-FGF15 signaling explains bile acids dysregulation in experimental colitis-associated colon cancer. Oncotarget, 8(38), 63665-63679.

+ Open protocol

+ Expand

Quantitative Bile Acid Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cholic acid (CA), chenodeoxy Cholic acid (CDCA), deoxyCholic acid (DCA), lithoCholic acid (LCA), α muriCholic acid (αMCA), βMCA, ωMCA, ursodeoxyCholic acid (UDCA), hyodeoxyCholic acid (HDCA), murideoxyCholic acid (MDCA), taurine conjugated Cholic acid (T-CA), T-CDCA, T-DCA, T-LCA, T- αMCA, T- βMCA, T- ωMCA, T-UDCA, and T-HDCA were purchased from Steraloids (Newport, Rhode Island). T- ωMCA was given by Dr. Daniel Raftery’s laboratory at the University of Washington Northwest Metabolomics Research Center. For internal standards (ISs) , a total of 5 deuterated (d4) ISs were used. Among them the d4-Cholic acid (d4-CA) was purchased from Toronto Research Chemicals Inc (Ontario, Canada), the d4-deoxyCholic acid (d4-DCA) and d4-chenodeoxyCholic acid (d4-CDCA) were purchased from CDN Isotope Inc (Pointe-Claire, Quebec, Canada), the d4-glycine conjugated CDCA (d4-G-CDCA) was purchased from IsoSciences (Ambler, PA), and d4-lithoCholic acid (d4-LCA) was purchased from Steraloids (Newport, Rhode Island). One mg/mL stock solutions of the individual BAs (for standard curve) and IS were prepared in methanol and water (1:1). The 19 individual BA stock solutions were further diluted in 50% methanol to obtain 10 working standard solutions ranging from 0.05 to 10000 ng/mL). The 5 ISs were mixed to obtain a working IS solution in which the concentration of each IS was 10 µmol/mL.

Kim S., Choi S., Dutta M., Asubonteng J.O., Polunas M., Goedken M., Gonzalez F.J., Cui J.Y, & Gyamfi M.A. (2021). Pregnane X receptor exacerbates nonalcoholic fatty liver disease accompanied by obesity- and inflammation-prone gut microbiome signature. Biochemical pharmacology, 193, 114698.

+ Open protocol

+ Expand

Bile Acid Panel Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

β-MCA, ω-MCA, UDCA, HDCA, T-α-MCA, T-β-MCA, T-UDCA, and THDCA were purchased from Steraloids Inc. (Newport, RI). α-MCA, CA, DCA, CDCA, TCDCA, TDCA, and TCA were purchased from Sigma-Aldrich (St Louis, MO). Irinotecan was obtained from Sigma-Aldrich (St Louis, MO). All other reagents and solvents were of HPLC grade. Anti-mouse CD3 (clone 145-2C11), anti-mouse CD28 (clone 37.51), anti-mouse CD16/CD32 (clone 93), anti-mouse CD45 Alexa Fluor® 700 (Clone: 30-F11), anti-mouse CD4 eFluor® 450 (Clone: GK1.5), anti-mouse CD8α PE-Cyanine7 (Clone: 53-6.7), anti-mouse CD8β FITC (Clone: eBioH35-17.2), anti-mouse TCRβ PerCP-Cyanine5.5 (Clone: H57-597), anti-mouse/rat Foxp3 APC (Clone: FJK-16s), anti-mouse IL-10 APC (Clone: JES5-16E3) were from eBiosciences (San Diego, CA). Cytofix/Cytoperm Fixation/ Permeabilization Solution and Golgi Plug protein transport inhibitor were purchased from BD Biosciences (Franklin Lakes, NJ). Foxp3 Fix/Perm Buffer Set and mouse IL-10 ELISA kit were purchased from BioLegend (San Diego, CA).

Fang Z.Z., Zhang D., Cao Y.F., Xie C., Lu D., Sun D.X., Tanaka N., Jiang C., Chen Q., Chen Y., Wang H, & Gonzalez F.J. (2015). Irinotecan (CPT-11)-induced elevation of bile acids potentiates suppression of IL-10 expression. Toxicology and applied pharmacology, 291, 21-27.

+ Open protocol

+ Expand

Comprehensive Bile Acid Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deuterated-cholic acid (d4-CA), bile acids (CA, CDCA, LCA, TCA, UDCA, taurine, βMCA, TαMCA, TβMCA, TLCA, TCDCA), and formic acid were purchased from Sigma-Aldrich Chemicals Co. (St Louis, MO, USA). All other bile acid standards (GCDCA, GCA, GDCA, GHDCA, GLCA, GUDCA, HCA, HDCA, ILA, TDCA, THDCA, TUDCA) were purchased from Steraloids Inc. (Newport, RI, USA). Acetonitrile (ACN) and methanol (MeOH) of Optima LC-MS grade quality were purchased from Thermo Fisher Scientific (Auckland, New Zealand).

James S.C., Fraser K., Young W., Heenan P.E., Gearry R.B., Keenan J.I., Talley N.J., Joyce S.A., McNabb W.C, & Roy N.C. (2021). Concentrations of Fecal Bile Acids in Participants with Functional Gut Disorders and Healthy Controls. Metabolites, 11(9), 612.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!