Hs gapdh 1 sg quantitect primer assay

Manufactured by Qiagen

The Hs_GAPDH_1_SG QuantiTect Primer Assay is a set of pre-designed and validated primers for the detection and quantification of the GAPDH gene in human samples using real-time PCR. The assay is optimized for use with the QuantiTect Primer Assay technology.

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Lab products found in correlation

Rt2 sybr green qpcr mastermix, by Qiagen (2 mentions) Rotor gene q, by Qiagen (2 mentions) Steponeplus real time pcr, by Thermo Fisher Scientific (1 mentions) Quantitect sybr green pcr kit, by Qiagen (1 mentions) Quantitect reverse transcription, by Qiagen (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Quantstudio 12k flex real time pcr system, by Thermo Fisher Scientific (1 mentions) Sybr green, by Thermo Fisher Scientific (1 mentions) First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Cfx96 system, by Bio-Rad (1 mentions)

Spelling variants of this product

QuantiTect Primer Assays (768 citations) QuantiTect primers (247 citations) QuantiTect (227 citations) GAPDH (170 citations) GAPDH primers (17 citations) Quantitect assays (5 citations) QuantiTect® Primer Assay Hs_GAPDH (4 citations)

5 protocols using hs gapdh 1 sg quantitect primer assay

RT-PCR Gene Expression Analysis

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The RT-PCR method was used for gene expression analysis and Rotor Gene Q (QIAGEN) was used for this purpose.
A primer pair specific to the SLC1A1 gene was used in the expression analysis, which was the Hs_SLC1A1_1_SG QuantiTect Primer Assay (QIAGEN). As an internal control, the housekeeping GAPDH gene and the primer pair specific to it (Hs_GAPDH_1_SG QuantiTect Primer Assay) (QIAGEN) was used. RT2 SYBR® Green qPCR Mastermix (QIAGEN) was used as premix for the gene expression analysis.

ERGÜN S., GÜNEŞ S., BÜYÜKALPELLİ R, & AYDIN O. (2019). Glutamate transporter SLC1A1 is associated with clear cell renal cell carcinoma. Turkish Journal of Medical Sciences, 49(2), 531-537.

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Quantifying DREAM mRNA Expression in Thyroid

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cDNA was generated using 1 ug of DNA-free RNA samples by QuantiTect Reverse Transcription (Qiagen) according to the manufacturer’s instructions. Quantitative real-time PCR measuring of DREAM mRNA was performed with commercially available assay primers (Hs_KCNIP3_1_SG QuantiTect primer assay, NM_001034914, NM_013434, Qiagen) in a PCR assay buffer that contained SYBR Green as fluorescent dye (QuantiTect SYBR Green PCR kit, Qiagen) per the manufacturer’s instructions. Fluorescence was detected using the Step One Plus™ Real-Time PCR (Applied Biosystems) system. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Hs_GAPDH_1_SG QuantiTect primer assay, NM_001256799, NM_002046, Qiagen) was used as the endogenous normalizing gene. A commercial pool of RNA from 47 normal human thyroid tissues (ages, 8–78 years) was used for comparisons (CLONTECH, BioChain, and Ambion). Relative quantification was performed by 2^−ΔΔCT method [14 (link)]. Over- and under-expression were defined as a 2-fold increase and decrease in comparison to normal thyroid tissue, respectively.

Shinzato A., Lerario A.M., Lin C.J., Danilovic D.S., Marui S, & Trarbach E.B. (2015). Evaluation of Downstream Regulatory Element Antagonistic Modulator Gene in Human Multinodular Goiter. Medical Science Monitor Basic Research, 21, 179-182.

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Quantification of ADRB1 and ADRB2 mRNA

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RNA was extracted by the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and 1 μg of RNA was converted into cDNA using a first‐strand cDNA synthesis kit (Thermo Fisher Scientific) in a Swift MiniPro Thermal Cycler (Esco LifeSciences, Changi, Singapore). Primer sequences were as follows: ADRB1: forward (5'‐CTG AGG GAT TTC TAC CTC ACA C‐3') and reverse (5'‐GCC TGG TCC TTC CAA CTA AT‐3'); ADRB2: forward (5'‐GAG CCT GCT GAC CAA GAA TAA‐3') and reverse (5'‐GAA TGG GCA AGA AGG TAA‐3'). ADRB1 and ADRB2 primers were obtained from Integrated DNA Technologies (Leuven, Belgium). SYBR Green (Applied Biosystems, Thermo Fisher Scientific) was used to perform quantitative real‐time PCR. Expression levels of mRNA were determined using the QuantStudio 12K Flex Real‐Time PCR System (Thermo Fisher Scientific). Each sample was amplified in triplicate. Transcripts of GAPDH (Hs_GAPDH_1_SG Quantitect Primer Assay, Qiagen) were measured for normalization.

Satilmis H., Verheye E., Vlummens P., Oudaert I., Vandewalle N., Fan R., Knight J.M., De Beule N., Ates G., Massie A., Moreaux J., Maes A., De Bruyne E., Vanderkerken K., Menu E., Sloan E.K, & De Veirman K. (2022). Targeting the β2‐adrenergic receptor increases chemosensitivity in multiple myeloma by induction of apoptosis and modulating cancer cell metabolism. The Journal of Pathology, 259(1), 69-80.

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Quantitative Gene Expression Analysis

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RT-PCR method was used for gene expression analysis, and Rotor Gene Q (Qiagen GmbH) was used for this purpose.
Primer pairs specific to four genes (ATXN3, ABI2, GOLGB1 and SMAD2) were used in the expression analysis: Hs_ATXN3_1_SG QuantiTect Primer Assay, Hs_ABI2_1_SG QuantiTect Primer Assay, Hs_GOLGB1_1_SG QuantiTect Primer Assay and Hs_SMAD2_1_SG QuantiTect Primer Assay (Qiagen GmbH) respectively. As an internal control, the housekeeping GAPDH gene and the primer pair specific to it (Hs_GAPDH_1_SG QuantiTect Primer Assay) (Qiagen GmbH) were used. 21 RT2 SYBR® Green qPCR Mastermix (Qiagen GmbH) was used as premix for gene expression analysis. RT-PCR reaction mixture was prepared according to the kit protocol. Mixtures were transferred to 25 µL tubes, and the reaction conditions specified by the kit were applied.

Ergun S., Gunes S., Buyukalpelli R, & Aydin O. (2020). Association of Abl interactor 2, ABI2, with platelet/lymphocyte ratio in patients with renal cell carcinoma: A pilot study. International journal of experimental pathology, 101(3-4).

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Quantitative Gene Expression Analysis

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Gene expression of MMP13, GAPDH, and ACTA2 was assessed using validated human primers: MMP13 Human PrimePCR™SYBR^® Green, Assay ID: qHsaCIP0026824 (Biorad); Hs_GAPDH_1_SG QuantiTect Primer Assay ID: QT00079247 (Qiagen) and primer sequences for ACTA2: forward, 5′-GGAATGGGACAAAAAGACAGCTA-3′; reverse, 5′-CGGGTACTTCAGGGTCAGGAT-3′. Comparative real-time PCR was performed in triplicate, including no-template controls. Real-time analysis was performed on a CFX96 System (Biorad, Hercules, CA, USA), and the 2^−ΔΔCt method was used to assess the relative expression.

Mancarella S., Serino G., Coletta S., Armentano R., Dituri F., Ardito F., Ruzzenente A., Fabregat I, & Giannelli G. (2022). The Tumor Microenvironment Drives Intrahepatic Cholangiocarcinoma Progression. International Journal of Molecular Sciences, 23(8), 4187.

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