MH7A cells were obtained from Riken Cell Bank (Saitama, Japan) and cultured in RPMI 1640 medium (Thermo Fisher Scientific, Tokyo, Japan) supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) and 4 × Antibiotic–Antimycotic liquid (Thermo Fisher Scientific) at 37 °C under an atmosphere of 5% CO2.
Mh7a cells
Manufactured by RIKEN Cell Bank
Sourced in Japan
The MH7A cells are a mouse cell line derived from synovial tissue. They are adherent cells that can be used for in vitro studies. The core function of the MH7A cells is to serve as a model system for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
1640 medium, by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic liquid, by Thermo Fisher Scientific (1 mentions)
Raw 264.7 cell, by Korean Cell Line Bank (1 mentions)
Fetal bovine serum (fbs), by Korean Cell Line Bank (1 mentions)
1640 medium, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
8 protocols using mh7a cells
1
Cell Culture Protocol for MH7A Cells
2
MH7A Cell Culture Protocol
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MH7A cells were obtained from the Riken Cell Bank (Tsukuba, Japan). Cells were cultured in a mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 medium (DMEM/F12) supplemented with 10% fetal bovine serum (FBS) in a CO2 incubator at 37°C. All cell culture reagents were obtained from Gibco (Thermo Fisher Scientific, MA, United States).
3
Cell culture protocol for MH7A and Raw 264.7 cells
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MH7A cells were purchased from Riken Cell Bank (Tsukuba, Japan) and cultured in RPMI1640 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% penicillin/streptomycin. Raw 264.7 cells were purchased from the Korean Cell Line Bank and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin. FBS and all other reagents used for cell culture were purchased from Invitrogen (Carlsbad, CA, USA). The cultures were maintained at 37°C in an incubator with a controlled humidified atmosphere composed of 95% air and 5% CO2.
4
MH7A Cell Culture Protocol
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MH7A cells were obtained from the Riken cell bank (Tsukuba, Japan). Cells were maintained in Roswell Park Memorial Institute 1640 medium (Sigma-Aldrich), supplemented with 10% fetal bovine serum (Sigma-Aldrich) and penicillin/streptomycin (1:100; Sigma-Aldrich), in a CO2 incubator at 37°C.
5
Culturing MH7A Cells in RPMI 1640
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MH7A cells were obtained from the Riken Cell Bank (Tsukuba, Japan). They were cultured in the Roswell Park Memorial Institute (RPMI) 1640 medium (Hyclone, Thermo Fisher Scientific, Wilmington, DE, USA), with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific), and 1% penicillin/streptomycin (Sigma–Aldrich, MO, USA). Cell lines were cultured in an incubator at the constant temperature of 37 °C, constant relative humidity, and 5% CO2.
6
Quercetin Modulation of RA Fibroblast-like Synoviocytes
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Human RA fibroblast-like synoviocyte cell line (MH7A cells) was purchased from the Riken cell bank (Ibaraki, Japan), and cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution (HyClone, Shanghai). Subsequently, the MH7A cells were plated into a six-well plate (5 × 103 cells/well) and treated with different concentrations of quercetin (0, 25, 50, 75, and 100 μM; Sigma, St Louis, MO, United States) in 37°C incubator with 5% CO2 atmosphere for 24 h. Meanwhile, the culture medium with 10% DMEM and 0.05% DMSO but without MH7A cells was set as control groups and also plated in the same condition for 24 h.
7
Cell Culture Protocol for MH7A and EA.hy926
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MH7A cells were purchased from the Riken Cell Bank (Tsukuba, Japan). The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Hyclone, Thermo Fisher Scientific, Wilmington, DE, USA), with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific), and 1% penicillin/streptomycin (Sigma–Aldrich, MO, USA). EA.hy926 cells were cultured in Dulbecco Modified Eagle Medium (DMEM) medium with high glucose, supplemented with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin/streptomycin (Sigma–Aldrich). All cell lines were cultured in an incubator with the constant temperature of 37°C, constant humidity, and 5% of CO2.
8
Culture of Immortalized Rheumatoid Arthritis Synovial Fibroblasts
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Immortalized RASFs (MH7A cells) were bought from the Riken Cell Bank (Ibaraki, Japan) and cultured as a single culture in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin 100 U/mL. Cell incubation was performed in a humidified atmosphere of 37°C, 5% CO2. The cells were passaged when they had grown to 80% confluence.
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