C kit apc 2b8

Manufactured by BD

Sourced in United States, Germany

The C-Kit-APC (2B8) is a lab equipment product used for detecting and analyzing the expression of the c-Kit receptor on cell surfaces. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the c-Kit receptor, also known as CD117. The C-Kit-APC (2B8) can be used in flow cytometry analysis to identify and quantify c-Kit-positive cells.

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Lab products found in correlation

Cd11b apc m1 70, by Thermo Fisher Scientific (2 mentions) F4 80 pe bm8, by Thermo Fisher Scientific (2 mentions) Edta microtainers, by BD (1 mentions) Sca 1 pe cy7 d7, by BD (1 mentions) Streptavidin apc cy7, by BD (1 mentions) Immersol medium, by Zeiss (1 mentions) Cd45 fitc hi30, by BD (1 mentions) Facscalibur, by BD (1 mentions)

Close spelling variants of this product

Anti-c-Kit-APC (2B8)-APC APC-conjugated anti-c-Kit (2B8, C-Kit-APC-eFlour780 (2B8), C-Kit (2B8, C-Kit-APC

3 protocols using c kit apc 2b8

Comprehensive Hematopoietic Lineage Staining

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Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), B220-PE (RA3-6B2), Sca-1-PE (D7), ckit-APC (2B8) and CD45-FITC (HI30) from BD Biosciences, CD11b-APC (M 1/70) and F4/80-PE (BM8) from eBioscience. Antibodies used for the lineage cocktail were PerCP Cy5.5 labelled CD4 (GK1.5) and Ter119 (Ter-119) from Biolegend, B220 (RA3-6B2) and CD3 (145-2C11) from BD Biosciences and Gr-1 (RB6-8C5C), CD8 (53-6.7), IL7R (A7R34), CD11b (M1/70) from eBioscience. Antibodies used for SLAM HSC staining were CD48-APC (HM48-1), CD150-PECy7 (TC-15-12F12.2) from Biolegend and CD244-PE (2B4) from eBioscience. Antibodies used for progenitor staining were CD16/32-PE (93), CD34-biotin (RAM34), Sca1-PECy7 (D7) from eBioscience, ckit-APC (2B8) and Streptavidin-APC-Cy7 from BD Biosciences. Cytospin preparations were stained with Wright-Giemsa stain. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40x/0.75 numerical aperture objective, or a 100x/1.3 numerical aperture objective with Zeiss immersol medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.

Chaturvedi A., Araujo Cruz M.M., Jyotsana N., Sharma A., Goparaju R., Schwarzer A., Görlich K., Schottmann R., Struys E.A., Jansen E.E., Rohde C., Müller-Tidow C., Geffers R., Göhring G., Ganser A., Thol F, & Heuser M. (2016). Enantiomer-specific and paracrine leukemogenicity of mutant IDH metabolite 2-hydroxyglutarate. Leukemia, 30(8), 1708-1715.

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Multidimensional Immune Profiling Post-Transplant

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Blood collection began 4 weeks post-transplant through retro-orbital puncture, collected into EDTA Microtainers (BD Biosciences, San Jose, CA, USA), and stained as previously described.²⁷ (link) Antibodies used were mouse CD45.1/CD45.2-V500 (30-F11), CD3-Pacific Blue (17A2), CD4-allophycocyanin (APC) (RM4-5), CD8-peridinin-chlorophyll-protein (PerCP) (53-6.7), CD14-phycoerythrin (PE) (rmC5-3), CD19-APC-H7 (1D3), Lineage-V450, c-Kit-APC (2B8), Sca1-PE-Cy7 (D7), CD150-PerCP/Cy5.5 (TC15-12F12.2), and CD48-APC-Cy7 (HM48-1) (BD Biosciences, BioLegend [San Diego, CA, USA], eBioscience [Waltham, MA, USA]). Lineage subsets were gated based on side scatter with CD45 on the x axis (Figure S4). Lymphocyte subsets were further analyzed based on surface markers (Figure S4). DNA was extracted (QIAGEN, Hilden, Germany) and analyzed as previously described.²³ (link)

Srikanthan M.A., Humbert O., Haworth K.G., Ironside C., Rajawat Y.S., Blazar B.R., Palchaudhuri R., Boitano A.E., Cooke M.P., Scadden D.T, & Kiem H.P. (2020). Effective Multi-lineage Engraftment in a Mouse Model of Fanconi Anemia Using Non-genotoxic Antibody-Based Conditioning. Molecular Therapy. Methods & Clinical Development, 17, 455-464.

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Lineage Determination of Murine Hematopoietic Cells

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Cells were plated at a density of 5 × 10⁵ cells/ml in flat-bottom 6-well plates (5 ml/well) under light-protective conditions and vitC was added at the specified concentrations. Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), Sca1-PE (D7), ckit-APC (2B8; BD Biosciences, Heidelberg, Germany), CD11b-APC (M1/70) and F4/80-PE (BM8; eBioscience, Frankfurt, Germany). Lineage distribution was determined by fluorescence-activated cell sorting analysis (FACSCalibur, Becton Dickinson, Heidelberg, Germany) as previously described.^{9 (link)}

Mingay M., Chaturvedi A., Bilenky M., Cao Q., Jackson L., Hui T., Moksa M., Heravi-Moussavi A., Humphries R.K., Heuser M, & Hirst M. (2017). Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia. Leukemia, 32(1), 11-20.

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