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C kit apc 2b8

Manufactured by BD
Sourced in United States, Germany

The C-Kit-APC (2B8) is a lab equipment product used for detecting and analyzing the expression of the c-Kit receptor on cell surfaces. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the c-Kit receptor, also known as CD117. The C-Kit-APC (2B8) can be used in flow cytometry analysis to identify and quantify c-Kit-positive cells.

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3 protocols using c kit apc 2b8

1

Comprehensive Hematopoietic Lineage Staining

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Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), B220-PE (RA3-6B2), Sca-1-PE (D7), ckit-APC (2B8) and CD45-FITC (HI30) from BD Biosciences, CD11b-APC (M 1/70) and F4/80-PE (BM8) from eBioscience. Antibodies used for the lineage cocktail were PerCP Cy5.5 labelled CD4 (GK1.5) and Ter119 (Ter-119) from Biolegend, B220 (RA3-6B2) and CD3 (145-2C11) from BD Biosciences and Gr-1 (RB6-8C5C), CD8 (53-6.7), IL7R (A7R34), CD11b (M1/70) from eBioscience. Antibodies used for SLAM HSC staining were CD48-APC (HM48-1), CD150-PECy7 (TC-15-12F12.2) from Biolegend and CD244-PE (2B4) from eBioscience. Antibodies used for progenitor staining were CD16/32-PE (93), CD34-biotin (RAM34), Sca1-PECy7 (D7) from eBioscience, ckit-APC (2B8) and Streptavidin-APC-Cy7 from BD Biosciences. Cytospin preparations were stained with Wright-Giemsa stain. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40x/0.75 numerical aperture objective, or a 100x/1.3 numerical aperture objective with Zeiss immersol medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.
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2

Multidimensional Immune Profiling Post-Transplant

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Blood collection began 4 weeks post-transplant through retro-orbital puncture, collected into EDTA Microtainers (BD Biosciences, San Jose, CA, USA), and stained as previously described.27 (link) Antibodies used were mouse CD45.1/CD45.2-V500 (30-F11), CD3-Pacific Blue (17A2), CD4-allophycocyanin (APC) (RM4-5), CD8-peridinin-chlorophyll-protein (PerCP) (53-6.7), CD14-phycoerythrin (PE) (rmC5-3), CD19-APC-H7 (1D3), Lineage-V450, c-Kit-APC (2B8), Sca1-PE-Cy7 (D7), CD150-PerCP/Cy5.5 (TC15-12F12.2), and CD48-APC-Cy7 (HM48-1) (BD Biosciences, BioLegend [San Diego, CA, USA], eBioscience [Waltham, MA, USA]). Lineage subsets were gated based on side scatter with CD45 on the x axis (Figure S4). Lymphocyte subsets were further analyzed based on surface markers (Figure S4). DNA was extracted (QIAGEN, Hilden, Germany) and analyzed as previously described.23 (link)
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3

Lineage Determination of Murine Hematopoietic Cells

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Cells were plated at a density of 5 × 105 cells/ml in flat-bottom 6-well plates (5 ml/well) under light-protective conditions and vitC was added at the specified concentrations. Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), Sca1-PE (D7), ckit-APC (2B8; BD Biosciences, Heidelberg, Germany), CD11b-APC (M1/70) and F4/80-PE (BM8; eBioscience, Frankfurt, Germany). Lineage distribution was determined by fluorescence-activated cell sorting analysis (FACSCalibur, Becton Dickinson, Heidelberg, Germany) as previously described.9 (link)
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