A 1H NMR spectrometer (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR; Varian Inc, Santa Clara, CA, USA) was used to monitor synthesis of HVGGSSV-chitoPEGAcHIS copolymers.
Unity inova 500 mhz nb high resolution ft nmr
Manufactured by Agilent Technologies
Sourced in United States
The Unity Inova 500 MHz NB High Resolution FT NMR is a nuclear magnetic resonance (NMR) spectrometer designed for high-resolution analysis. It operates at a frequency of 500 MHz and is capable of performing Fourier transform (FT) NMR experiments. The instrument is optimized for narrow-band (NB) applications.
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7 protocols using unity inova 500 mhz nb high resolution ft nmr
1
Synthesis Monitoring of HVGGSSV-chitoPEGAcHIS
2
Monitoring Synthesis via NMR Spectroscopy
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1H nuclear magnetic resonance (NMR) spectroscopy (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR; Varian Inc, Santa Clara, CA, USA) was used to monitor the synthesis procedure.
3
NMR and GPC Characterization of Polymers
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Characterization of the synthesized polymers was performed in CDCl3 by 500 mHz 1H-NMR spectroscopy (500 mHz superconducting FT-NMR spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR; Varian Inc, Santa Clara, CA, USA). Molecular weight of polymers was measured with the gel permeation chromatography (GPC) system (Waters 2690D-2410, Waters, MA, USA) as described previously [5 (link)]. GPC was equipped with a Waters 6000 solvent delivery system, RI detector, and a Rheodyne injector. Phenogel columns of 500, 103, 104, and 105 Å were used. A standard curve was established with standard polystyrene samples.
4
Synthesis and Characterization of ChitoPEGthDOX Conjugates
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ChitoPEGthDOX conjugates (20 mg) were reconstituted in deionized water (3 mL), and 5 mL DMSO was added. This was dialyzed against water for 24 h using a dialysis membrane (MWCO = 8000 g/mol) to remove the organic solvent. The resulting solution was then used for analysis or experiment.
1H nuclear magnetic resonance (NMR) spectroscopy (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High-Resolution FT NMR; Varian Inc., Santa Clara, CA, USA) was employed to confirm the synthesis procedure. Synthesized chemicals were dissolved in D2O, DMSO, or D2O/DMSO mixtures.
The morphology of the nanoparticles was observed with a transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan). An aqueous solution of ChitoPEGthDOX (nanoparticle weight 1 mg/mL) was dropped onto a carbon film-coated copper grid. This was negatively stained with phosphotungstic acid (0.1%, w/w in H2O). The observation was carried out at 80 kV and 20 °C.
An aqueous nanoparticle solution was used to analyze the particle size distribution using Nano-ZS (Malvern, Worcestershire, UK) at 20 °C. The nanoparticle concentration was less than 0.1 wt-%.
1H nuclear magnetic resonance (NMR) spectroscopy (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High-Resolution FT NMR; Varian Inc., Santa Clara, CA, USA) was employed to confirm the synthesis procedure. Synthesized chemicals were dissolved in D2O, DMSO, or D2O/DMSO mixtures.
The morphology of the nanoparticles was observed with a transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan). An aqueous solution of ChitoPEGthDOX (nanoparticle weight 1 mg/mL) was dropped onto a carbon film-coated copper grid. This was negatively stained with phosphotungstic acid (0.1%, w/w in H2O). The observation was carried out at 80 kV and 20 °C.
An aqueous nanoparticle solution was used to analyze the particle size distribution using Nano-ZS (Malvern, Worcestershire, UK) at 20 °C. The nanoparticle concentration was less than 0.1 wt-%.
5
Characterization of PEI/NONOate Nanoparticles
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The nuclear magnetic resonance (NMR) spectra of the PEI/NONOate were recorded in deuterium oxide (D2O) using 1H-NMR spectroscopy (500 MHz superconducting FT-NMR spectrometer, Unity Inova 500 MHz NB high resolution FT-NMR, Varian Inc, Santa Clara, CA, USA). The FTIR spectrum of the PEI/NONOate was measured using a Varian® 640 FT-IR spectrophotometer (Varian Inc) in transmittance mode with potassium bromide (KBr) plates. A total of 48 scans were taken per spectrum in the range from 4,000 to 500 cm−1 at a resolution of 4 cm−1. For the UV-Vis spectral analysis, different concentrations of the PEI/NONOate were measured in Dulbecco’s phosphate-buffered saline (DPBS) at 37°C using a UV-Vis spectrophotometer (Optizen 2120 UV, Mecasys, Republic of Korea) at predetermined time intervals. For nitrite (NO2−) analysis, Griess reagent was added to the PEI/NONOate in DPBS at 37°C and nitrite was spectrophotometrically detected by monitoring the formation of an azo dye.
6
ICI Nanocomposites Structural Analysis
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The
chemical structure of ICI nanocomposites was confirmed with 13C NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR
spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR;
Varian Inc, Santa Clara, CA).
chemical structure of ICI nanocomposites was confirmed with 13C NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR
spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR;
Varian Inc, Santa Clara, CA).
7
Characterization of Ce6-HA Nanophotosensitizers
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The synthesis of Ce6tetraHA/Ce6decaHA conjugates was confirmed with 1H NMR spectra (500 mHz superconducting Fourier transform (FT)-NMR spectrometer, Varian Unity Inova 500 MHz NB High Resolution FT NMR; Varian Inc., Santa Clara, CA, USA).
The particle size of Ce6tetraHA or Ce6decaHA nanophotosensitizers (concentration: 0.1%, w/w) was analyzed with Zetasizer Nano-ZS (Malvern, Worcestershire, UK). To estimate average particle size, they were measured particle sizes at least three times and expressed as average ± S.D.
The morphology of Ce6tetraHA nanophotosensitizers was observed with transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan). One drop of nanophotosensitizer solution was put onto the carbon film coated grid. Subsequently, this was dried in room temperature for 3 h, followed by negative staining with phosphotungstic acid (0.1%, w/w in deionized water). The observation of nanophotosensitizer morphology was performed at 80 kV.
The particle size of Ce6tetraHA or Ce6decaHA nanophotosensitizers (concentration: 0.1%, w/w) was analyzed with Zetasizer Nano-ZS (Malvern, Worcestershire, UK). To estimate average particle size, they were measured particle sizes at least three times and expressed as average ± S.D.
The morphology of Ce6tetraHA nanophotosensitizers was observed with transmission electron microscope (TEM) (H-7600, Hitachi Instruments Ltd., Tokyo, Japan). One drop of nanophotosensitizer solution was put onto the carbon film coated grid. Subsequently, this was dried in room temperature for 3 h, followed by negative staining with phosphotungstic acid (0.1%, w/w in deionized water). The observation of nanophotosensitizer morphology was performed at 80 kV.
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