Ds ri2 color

For bacterial morphology observations, 1 μl of fresh culture was applied to an agarose pad on a slide and covered by a coverslip. Images were captured on a Nikon Eclipse 80i with Plan Fluor 100× oil Ph3 DLL objective and a Nikon DS-Ri2 color camera.

IHC of tumor sections was performed by Sophistolab (Muttenz, Switzerland) on a Lecia BondMax instrument using Refine HRP-Kits (Leica DS9800). All buffer-solutions were purchased from Lecia Microsystems Newcastle, Ltd and used according to the manufacturer’s guidelines. Paraffin-slides were de-waxed, pre-treated and incubated as follows: ER-solution 2 for 10 min at 95 °C, ER-solution 2 for 20 min at 100 °C and ER-solution 2 for 30 min at 100 °C. pFRS2; Rabbit anti-Phospho FRS2 (PhosphoY 436, Abcam Limited; ab193363), dilution 1:150; phospho-p44/42 MAPK (Erk1/2_{Thr202/Tyr204}) Rabbit mAb (Cell Signaling Technology #4370), dilution 1:1600. The IHC images were captured digitally using a Nikon Epifluorescence Eclipse Ti2 equipped with a Nikon DS-Ri2 color and a Nikon DS-Qi2 monochrome camera.