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Bks cg m leprdb j db db mice

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BKS.Cg-m+/+Leprdb/J (db/db) mice are a type of laboratory mouse model. They are homozygous for the diabetes spontaneous mutation (Leprdb) and exhibit characteristics of type 2 diabetes, including obesity, hyperglycemia, and insulin resistance.

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8 protocols using bks cg m leprdb j db db mice

1

Exosome Treatment for Diabetic Mice

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All experimental procedures were carried out in accordance with the NIH Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital. Twenty-week-old male BKS.Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories, Bar Harbor, ME, USA) were used. Heterozygous mice (db/m), a non-penetrant genotype (Jackson Laboratories), of the same age, were used as the control groups. db/db mice were randomly divided into two groups according to a randomisation scheme: saline (0.9% NaCl) (db/db), and MSC-exosomes (db+exo) (n=8 per group). Blood glucose, HbA1c, total cholesterol and triacylglycerol levels were tested every two weeks. MSC-exosomes (1×109 particles per animal per injection) were administered via a tail vein weekly for 8 consecutive weeks. Functional tests were performed every two weeks as indicated in Fig. 2a. All animals were euthanised at week 8 after the initial treatment and tissues were collected for further analysis. Experimenters were not blind to group assignment and outcome assessment, and no mouse was excluded from the experiments.
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2

Diabetic Mouse Model Protocol

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All experimental procedures were carried out in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the institutional Animal Care and Use Committee of Henry Ford Hospital. Male BKS.Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories, USA) aged 24 weeks were used. Age-matched heterozygote mice (db/m), a nonpenetrant genotype (Jackson Laboratories), were used as the control animals.
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3

Diabetic Mouse Model Experiment

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All experimental procedures were carried out in accordance with NIH Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Henry Ford Hospital (IACUC#1398). Male BKS. Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories, Bar Harbor, Maine) aged 20 weeks were used. Age-matched heterozygote mice (db/m), a non-penetrant genotype (Jackson Laboratories), were used as the control animals.
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4

Diabetic Mouse Model Analysis

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All experimental procedures were carried out in accordance with NIH Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Henry Ford Hospital (IACUC #1185). Male BKS. Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories) aged 36 weeks were used. Age-matched male heterozygote mice (db/m), a non-penetrant genotype (Jackson Laboratories), were used as the control animals.
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5

Metabolic Phenotyping of Diabetic Mice

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All experimental procedures were carried out in accordance with NIH Guide for the Care and Use of Laboratory Animals and approved by the institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital. Male and female BKS.Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories, Bar Harbor, Maine) aged 20 and 30 weeks were used. Age-matched heterozygotes mice (db/m), a non-penetrant genotype, were used as the control animals.
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6

Exosome Therapy for Diabetic Mice

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All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital and conducted in strict accordance with NIH Guidelines for the Care and Use of Laboratory Animals. Male BKS.Cg-m+/+Leprdb/J (db/db) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Same aged heterozygote mice (dm) were used as the control. At the age of 20 weeks, db/db mice were randomly divided into the following groups according to a randomization scheme: saline (db/db+vehicle), naïve MSC-exosomes (db/db+exo-naïve), and exo-146a (db/db+exo-146a). N= 8 per group. Exo-naïve and exo-146a were injected via a tail vein once a week for four consecutive weeks. Db/db group and dm group were administrated with same volume of saline. Glycosylated hemoglobin (HbA1c), glucose (Glc), total cholesterol (TC), and triglyceride (TG) levels in blood were monitored weekly using Accu-check active test strips (Roche Diagnostics, Basel, Switzerland). Serum insulin was tested prior to and after exosome treatment using a mouse ELISA kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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7

Diabetes in db/db Mice

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All experimental procedures were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the institutional Animal Care and Use Committee of Henry Ford Hospital. Male BKS.Cg-m+/+Leprdb/J (db/db) mice (The Jackson Laboratory, Bar Harbor, ME) aged 20 weeks were used. Age-matched heterozygotes mice (db/m), a nonpenetrant genotype, were used as the control animals.
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8

Diabetic Neuropathy in db/db Mice

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DRG, sciatic nerve (SN) and foot pad (FP) tissues were harvested from BKS.Cg-m+/+Leprdb/J (db/db) mice (Jackson Laboratories, Bar Harbor, ME, USA) at the age of 20 weeks when mice exhibited peripheral neuropathy [17 (link)] (Fig s1). The db/db mouse has a point mutation in the leptin receptor gene causing severe depletion of the insulin-producing beta-cells of the pancreatic islets, hyperglycemia and eventually leading to diabetes [18 (link)]. The age-matched heterozygote mice (db/m) were used as control. Physiological parameters of db/m and db/db mice including blood glucose levels were measured (Fig s1).
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