Bks cg m leprdb j db db mice

Manufactured by Jackson ImmunoResearch

Sourced in United States

BKS.Cg-m+/+Leprdb/J (db/db) mice are a type of laboratory mouse model. They are homozygous for the diabetes spontaneous mutation (Leprdb) and exhibit characteristics of type 2 diabetes, including obesity, hyperglycemia, and insulin resistance.

Automatically generated - may contain errors

Lab products found in correlation

Age matched heterozygote mice db m, by Jackson ImmunoResearch (2 mentions) Mouse elisa kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Db/db mice (60 citations) BKS.Cg-m+/+Leprdb/J (16 citations) Leprdb/db (11 citations) BKS.Cg-m +/+ Leprdb (6 citations) Db/m mice (6 citations) Leprdb (db/db) mice (5 citations) BKS db/db mice (3 citations) Db/db (BKS.Cg-Leprdb/J) mice (2 citations) BKS.Cg-m +/+ Leprdb/J mice (2 citations)

8 protocols using bks cg m leprdb j db db mice

Exosome Treatment for Diabetic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with the NIH Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital. Twenty-week-old male BKS.Cg-m+/+Lepr^db/J (db/db) mice (Jackson Laboratories, Bar Harbor, ME, USA) were used. Heterozygous mice (db/m), a non-penetrant genotype (Jackson Laboratories), of the same age, were used as the control groups. db/db mice were randomly divided into two groups according to a randomisation scheme: saline (0.9% NaCl) (db/db), and MSC-exosomes (db+exo) (n=8 per group). Blood glucose, HbA_1c, total cholesterol and triacylglycerol levels were tested every two weeks. MSC-exosomes (1×10⁹ particles per animal per injection) were administered via a tail vein weekly for 8 consecutive weeks. Functional tests were performed every two weeks as indicated in Fig. 2a. All animals were euthanised at week 8 after the initial treatment and tissues were collected for further analysis. Experimenters were not blind to group assignment and outcome assessment, and no mouse was excluded from the experiments.

Fan B., Li C., Szalad A., Wang L., Pan W., Zhang R., Chopp M., Zhang Z.G, & Liu X.S. (2019). Mesenchymal stromal cell-derived exosomes ameliorate peripheral neuropathy in a mouse model of diabetes. Diabetologia, 63(2), 431-443.

+ Open protocol

+ Expand

Diabetic Mouse Model Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the institutional Animal Care and Use Committee of Henry Ford Hospital. Male BKS.Cg-m^+/+Lepr^db/J (db/db) mice (Jackson Laboratories, USA) aged 24 weeks were used. Age-matched heterozygote mice (db/m), a nonpenetrant genotype (Jackson Laboratories), were used as the control animals.

Wang L., Chopp M., Jia L., Lu X., Szalad A., Zhang Y., Zhang R, & Zhang Z.G. (2015). Therapeutic Benefit of Extended Thymosin β4 Treatment Is Independent of Blood Glucose Level in Mice with Diabetic Peripheral Neuropathy. Journal of Diabetes Research, 2015, 173656.

+ Open protocol

+ Expand

Diabetic Mouse Model Experiment

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with NIH Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Henry Ford Hospital (IACUC#1398). Male BKS. Cg-m+/+Lepr^db/J (db/db) mice (Jackson Laboratories, Bar Harbor, Maine) aged 20 weeks were used. Age-matched heterozygote mice (db/m), a non-penetrant genotype (Jackson Laboratories), were used as the control animals.

Wang L., Chopp M., Szalad A., Lu X., Jia L., Lu M., Zhang R.L, & Zhang Z.G. (2016). Tadalafil Promotes the Recovery of Peripheral Neuropathy in Type II Diabetic Mice. PLoS ONE, 11(7), e0159665.

+ Open protocol

+ Expand

Diabetic Mouse Model Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with NIH Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Henry Ford Hospital (IACUC #1185). Male BKS. Cg-m+/+Lepr^db/J (db/db) mice (Jackson Laboratories) aged 36 weeks were used. Age-matched male heterozygote mice (db/m), a non-penetrant genotype (Jackson Laboratories), were used as the control animals.

Wang L., Chopp M., Szalad A., Jia L., Lu X., Lu M., Zhang L., Zhang Y., Zhang R, & Zhang Z.G. (2015). Sildenafil Ameliorates Long Term Peripheral Neuropathy in Type II Diabetic Mice. PLoS ONE, 10(2), e0118134.

+ Open protocol

+ Expand

Metabolic Phenotyping of Diabetic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with NIH Guide for the Care and Use of Laboratory Animals and approved by the institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital. Male and female BKS.Cg-m+/+Lepr^db/J (db/db) mice (Jackson Laboratories, Bar Harbor, Maine) aged 20 and 30 weeks were used. Age-matched heterozygotes mice (db/m), a non-penetrant genotype, were used as the control animals.

Fan B., Liu X.S., Szalad A., Wang L., Zhang R., Chopp M, & Zhang Z.G. (2018). Influence of Sex on Cognition and Peripheral Neurovascular Function in Diabetic Mice. Frontiers in Neuroscience, 12, 795.

+ Open protocol

+ Expand

Exosome Therapy for Diabetic Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Henry Ford Hospital and conducted in strict accordance with NIH Guidelines for the Care and Use of Laboratory Animals. Male BKS.Cg-m+/+Leprdb/J (db/db) mice were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Same aged heterozygote mice (dm) were used as the control. At the age of 20 weeks, db/db mice were randomly divided into the following groups according to a randomization scheme: saline (db/db+vehicle), naïve MSC-exosomes (db/db+exo-naïve), and exo-146a (db/db+exo-146a). N= 8 per group. Exo-naïve and exo-146a were injected via a tail vein once a week for four consecutive weeks. Db/db group and dm group were administrated with same volume of saline. Glycosylated hemoglobin (HbA1c), glucose (Glc), total cholesterol (TC), and triglyceride (TG) levels in blood were monitored weekly using Accu-check active test strips (Roche Diagnostics, Basel, Switzerland). Serum insulin was tested prior to and after exosome treatment using a mouse ELISA kit (Thermo Fisher Scientific) according to the manufacturer’s protocol.

Fan B., Chopp M., Zhang Z.G, & Liu X.S. (2021). Treatment of diabetic peripheral neuropathy with engineered mesenchymal stromal cell-derived exosomes enriched with microRNA-146a provide amplified therapeutic efficacy. Experimental neurology, 341, 113694.

+ Open protocol

+ Expand

Diabetes in db/db Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the institutional Animal Care and Use Committee of Henry Ford Hospital. Male BKS.Cg-m^+/+Lepr^db/J (db/db) mice (The Jackson Laboratory, Bar Harbor, ME) aged 20 weeks were used. Age-matched heterozygotes mice (db/m), a nonpenetrant genotype, were used as the control animals.

Liu X.S., Fan B., Szalad A., Jia L., Wang L., Wang X., Pan W., Zhang L., Zhang R., Hu J., Zhang X.M., Chopp M, & Zhang Z.G. (2017). MicroRNA-146a Mimics Reduce the Peripheral Neuropathy in Type 2 Diabetic Mice. Diabetes, 66(12), 3111-3121.

+ Open protocol

+ Expand

Diabetic Neuropathy in db/db Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

DRG, sciatic nerve (SN) and foot pad (FP) tissues were harvested from BKS.Cg-m^+/+Lepr^db/J (db/db) mice (Jackson Laboratories, Bar Harbor, ME, USA) at the age of 20 weeks when mice exhibited peripheral neuropathy [17 (link)] (Fig s1). The db/db mouse has a point mutation in the leptin receptor gene causing severe depletion of the insulin-producing beta-cells of the pancreatic islets, hyperglycemia and eventually leading to diabetes [18 (link)]. The age-matched heterozygote mice (db/m) were used as control. Physiological parameters of db/m and db/db mice including blood glucose levels were measured (Fig s1).

Jia L., Chopp M., Wang L., Lu X., Zhang Y., Szalad A, & Zhang Z.G. (2018). MiR-34a Regulates Axonal Growth of Dorsal Root Ganglia Neurons by Targeting FOXP2 and VAT1 in Postnatal and Adult Mouse. Molecular neurobiology, 55(12), 9089-9099.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!